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J Appl Physiol (February 8, 2002). doi:10.1152/japplphysiol.00755.2001
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Articles in PresS, published online ahead of print February 8, 2002
J Appl Physiol, 10.1152/jap.00755.2001
Submitted on July 23, 2001
Accepted on February 4, 2002

Effects of albuterol enantiomers on ciliary beat frequency in ovine tracheal epithelial cells

Jeffrey I Frohock1, Corinne Wijkstrom-Frei1, and Matthias Salathe1*

1 Medicine/Pulmonary, University of Miami, Miami, FL, USA

* To whom correspondence should be addressed. E-mail: msalathe{at}miami.edu.

ß2-adrenergic agonists stimulate ciliary beat frequency (CBF), an integral part of mucociliary clearance. To evaluate the differential effects of albuterol enantiomers and their racemic mixture on ciliary function, CBF and intracellular calcium were measured at room temperature from single ovine airway epithelial cells using digital video microscopy. Baseline CBF was 7.2 ± 0.2 Hz (mean ± SE; n = 80). (R)-albuterol (10 µM - 1 mM), stimulated CBF in a dose-dependent manner to maximally 24.4 ± 5.4% above baseline. Racemic albuterol stimulated CBF to maximally 12.8 ± 3.6% above baseline, a significantly lower increase compared to (R)-albuterol alone, despite identical (R)-enantiomer concentrations in both groups. Simultaneous recordings of intracellular calcium and CBF from single cells indicated that the CBF increase in response to (R)-albuterol was mediated through ß-receptors and stimulation of protein kinase A, in both a calcium-dependent and -independent fashion. (S)-albuterol had a negligible effect on CBF and did not change intracellular calcium. Together, these results suggest that (R)-albuterol is more efficacious than racemic albuterol in stimulating CBF and thus, (S)-albuterol may interfere with the ability of (R)-albuterol to increase CBF.




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