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J Appl Physiol (January 18, 2002). doi:10.1152/japplphysiol.00742.2001
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Articles in PresS, published online ahead of print January 18, 2002
J Appl Physiol, 10.1152/jap.00742.2001
Submitted on July 20, 2001
Accepted on January 10, 2002

Effect of microgravity on the expression of myogenic factors during postnatal development of rat skeletal muscle

Manabu Inobe1, Ikuko Inobe2, Gregory R Adams3, Kenneth M Baldwin3, and Shin'ichi Takeda2*

1 Department of Molecular Therapy, National Institute of Neuroscience, NCNP, Kodaira, Tokyo, Japan; Department of Space Experiment, National Space Development Agency of Japan, Tsukuba, Ibaraki, Japan
2 Department of Molecular Therapy, National Institute of Neuroscience, NCNP, Kodaira, Tokyo, Japan
3 Department of Physiology and Biophysics, University of California, Irvine, Irvine, California, USA

* To whom correspondence should be addressed. E-mail: takeda{at}ncnp.go.jp.

To clarify the role of gravity in the postnatal development of skeletal muscle, we exposed neonatal rats at 7 days of age to microgravity. After 16 days of space flight, tibialis anterior, plantaris, medial gastrocnemius and soleus muscles were removed from the hind-limb musculature, and examined for the expression of MyoD-family transcription factors such as MyoD, myogenin and MRF4. For this purpose, we established a unique semi-quantitative method based on RT-PCR using specific primers tagged with infrared fluorescence. The relative expression of MyoD in the tibialis anterior and plantaris muscles, and that of myogenin in the plantaris and soleus muscles were significantly reduced (P<0.001) in the flight animals. In contrast, MRF4 expression was not changed in any muscle. These results suggest that MyoD and myogenin, but not MRF4, are sensitive to gravity related stimuli in some skeletal muscles during postnatal development.




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