Journal of Applied Physiology
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J Appl Physiol (February 13, 2004). doi:10.1152/japplphysiol.00715.2003
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Submitted on July 10, 2003
Accepted on February 9, 2004

Injections of recombinant human erythropoietin (rHuEPO) increases lactate influx into erythrocytes

PHILIPPE CONNES1, CORINNE F CAILLAUD1*, JACQUES MERCIER2, DIDIER BOUIX2, and JEAN-FRANCOIS CASTIES3

1 Faculte des Sciences du Sport, EA 3759 Approche Bio-psycho-sociale du Dopage, MONTPELLIER, France
2 Service Central de Physiologie, Hopital Arnaud de Villeneuve, EA 701 Physiologie des Interactions, MONTPELLIER, France
3 Faculte des Sciences du Sport, EA 2991 Efficience et Deficience motrice, MONTPELLIER, France

* To whom correspondence should be addressed. E-mail: corinne.caillaud{at}univ-montp1.fr.

Previous studies showed that erythropoietin not only increases red blood cells production but is also essential in both the synthesis and the good functioning of several erythrocyte membrane proteins, including band 3. It is still unknown whether anion and/or H+ fluxes are modified by erythropoietin. This study aimed to evaluate the effect of recombinant human erythropoietin (rHuEPO) injections on lactate transport into erythrocytes via band 3 and H+-monocarboxylate transporter MCT-1, two proteins involved in lactate exchange. Nine athletes received subcutaneous rHuEPO (50 U kg-1 body mass 3 times a week for 4 weeks) and seven athletes received a saline solution (placebo group). All subjects were also supplemented with oral iron and vitamins B9 and B12. Lactate transport into erythrocytes was studied before and after the r-HuEPO treatment at different lactate concentrations (1.6, 8.1, 41 and 81.1 mM). After treatment, MCT-1 lactate uptake was increased at 1.6, 41 (p<0.01) and 81.1 mM [La] (p<0.001) although lactate uptake via band 3 and non-ionic diffusion was unchanged. MCT-1 maximal velocity (VmaxLa) increased in the rHuEPO group (p<0.05), reaching higher values than in the placebo group (p<0.05) after treatment. Our results show that rHuEPO injections increased MCT-1 lactate influx at low and high lactate concentrations. The increase in MCT-1 VmaxLa suggests that rHuEPO may stimulate MCT-1 synthesis during erythrocyte formation in bone marrow.




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