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J Appl Physiol (November 16, 2001). doi:10.1152/japplphysiol.00706.2001
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Articles in PresS, published online ahead of print November 16, 2001
J Appl Physiol, 10.1152/jap.00706.2001
Submitted on July 6, 2001
Accepted on November 10, 2001

The Effect of Exercise Intensity on 24 h Energy Expenditure and Nutrient Oxidation

Edward L Melanson1*, Teresa A Sharp1, Helen M Seagle1, Tracy J Horton1, William T Donahoo1, Gary K Grunwald1, Jere T Hamilton1, and James O Hill1

1 Center for Human Nutrition, University of Colorado Health Sciences Center, Denver, CO, USA

* To whom correspondence should be addressed. E-mail: ed.melanson{at}uchsc.edu.

The aim of this study was to determine the effects of exercise at different intensities on 24 h energy expenditure (EE) and substrate oxidation. 16 adults (8 M/8 F) were studied on 3 occasions [sedentary day (CON), a low-intensity exercise day (LI, 400 kcal at 40% of VO2max), and a high-intensity exercise day (HI, 400 kcal at 70% of VO2max)] using whole-room indirect calorimetry. 24 h EE and carbohydrate oxidation were significantly elevated on the exercise days (CON<LI=HI), but 24 h fat oxidation was not different across conditions. Muscle enzymatic profile was not consistently related to 24 h fat or carbohydrate oxidation. Upon further analysis, it was found that compared to males, females sustained slightly higher rates of 24 h fat oxidation (mg.kg FFM-1.min-1), and had a muscle enzymatic profile favoring fat oxidation. It is concluded that exercise intensity has no effect on 24 h EE or nutrient oxidation. Additionally, it appears that females may sustain slightly greater 24 h fat oxidation rates during waking and active periods of the day.




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