Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

J Appl Physiol (September 6, 2007). doi:10.1152/japplphysiol.00679.2007
This Article
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
103/5/1744    most recent
00679.2007v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Louis, E. S.
Right arrow Articles by Trappe, S. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Louis, E. S.
Right arrow Articles by Trappe, S. W.
Submitted on June 26, 2007
Accepted on August 28, 2007

Time Course of Proteolytic, Cytokine, and Myostatin Gene Expression After Acute Exercise in Human Skeletal Muscle

Emily S. Louis1, Ulrika Raue1, Yifan Yang1, Bozena Jemiolo1, and Scott W. Trappe1*

1 Human Performance Laboratory, Ball State University, Muncie, Indiana, United States

* To whom correspondence should be addressed. E-mail: strappe{at}bsu.edu.

The aim of this study was to examine the time course induction of select proteolytic (MuRF-1, atrogin-1, FOXO3A, calpain-1, calpain-2), myostatin, and cytokine (IL -6, -8, -15 and TNF{alpha}) mRNA after an acute bout of resistance (RE) or run (RUN) exercise. Six experienced RE (25 ± 4 yr, 74 ± 14 kg, 1.71 ± 0.11 m) and six RUN (25 ± 4 yr, 72 ± 5 kg, 1.81 ± 0.07 m) subjects had muscle biopsies from the vastus lateralis (RE) or gastrocnemius (RUN) before, immediately after, and 1, 2, 4, 8, 12, and 24 h post exercise. RE increased (p<0.05) mRNA expression of MuRF-1 early (3.5-fold, 1-4 h), followed by a decrease in atrogin-1 (3.3-fold) and FOXO3A (1.7-fold) 8-12 h post exercise. Myostatin mRNA decreased (6.3-fold; p<0.05) from 1-24 h post exercise, while IL-6, IL-8 and TNF{alpha} mRNA was elevated 2-12 h. RUN increased (p<0.05) MuRF-1 (3.6-fold), atrogin-1 (1.6-fold) and FOXO3A (1.9-fold) 1-4 h post exercise. Myostatin was suppressed (3.6-fold; p<0.05) 8-12 h post RUN. The cytokines exhibited a biphasic response, with immediate elevation (p<0.05) of IL-6, IL-8, and TNF{alpha}, followed by a second elevation (p<0.05) 2-24 h post exercise. In general, the timing of the gene induction indicated early elevation of proteolytic genes, followed by prolonged elevation of cytokines and suppression of myostatin. These data provide basic information for the timing of human muscle biopsy samples for gene expression studies involving exercise. Furthermore, this information suggests a greater induction of proteolytic genes following RUN as compared to RE.




This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
S. J. Kim, R. R. Roy, J. A. Kim, H. Zhong, F. Haddad, K. M. Baldwin, and V. R. Edgerton
Gene expression during inactivity-induced muscle atrophy: effects of brief bouts of a forceful contraction countermeasure
J Appl Physiol, October 1, 2008; 105(4): 1246 - 1254.
[Abstract] [Full Text] [PDF]

Home page
 Biol Res NursHome page
S. Al-Majid and H. Waters
The biological mechanisms of cancer-related skeletal muscle wasting: the role of progressive resistance exercise.
Biol Res Nurs, July 1, 2008; 10(1): 7 - 20.
[Abstract] [PDF]

Home page
 J. Appl. Physiol.Home page
H. D. Kollias and J. C. McDermott
Transforming growth factor-{beta} and myostatin signaling in skeletal muscle
J Appl Physiol, March 1, 2008; 104(3): 579 - 587.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
H. C. Dreyer, M. J. Drummond, B. Pennings, S. Fujita, E. L. Glynn, D. L. Chinkes, S. Dhanani, E. Volpi, and B. B. Rasmussen
Leucine-enriched essential amino acid and carbohydrate ingestion following resistance exercise enhances mTOR signaling and protein synthesis in human muscle
Am J Physiol Endocrinol Metab, February 1, 2008; 294(2): E392 - E400.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1948 by the American Physiological Society.