Journal of Applied Physiology
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J Appl Physiol (October 19, 2001). doi:10.1152/japplphysiol.00643.2000
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Articles in PresS, published online ahead of print October 19, 2001
J Appl Physiol, 10.1152/jap.00643.2000
Submitted on July 5, 2000
Accepted on October 15, 2001

Effects of Fatigue and Training on Sarcoplasmic Reticulum Ca2+ Regulation in Human Skeletal Muscle

Jia L Li1, Xiao N Wang2, Steve F Fraser1, Michael F Carey2, Tim V Wrigley1, and Michael J McKenna1*

1 Human Movement, Recreation and Performance, Victoria University of Technology, Melbourne, Victoria, Australia
2 Life Sciences and Technology, Victoria University of Technology, Melbourne, Victoria, Australia

* To whom correspondence should be addressed. E-mail: michael.mckenna{at}vu.edu.au.

Little is known about fatigue and training effects on sarcoplasmic reticulum (SR) function in human muscle, and we therefore investigated this in eight untrained controls (UT), eight endurance-trained (ET) and eight resistance-trained athletes (RT). Muscle biopsies (v.lateralis) taken at rest and after 50 maximal quadriceps contractions (180°/s, 0.5Hz) were analyzed for fiber composition, metabolites and maximal SR Ca2+ release, Ca2+ uptake and Ca2+ATPase activity. Fatigue reduced (P<0.05) Ca2+ release (42.1±3.8%, 43.4±3.9%, 31.3±6.1%), Ca2+ uptake (43.0±5.2%, 34.1±4.6%, 28.4±2.8%), and Ca2+ATPase activity (38.6±4.2%, 48.5±5.7%, 29.6±5.0%), in UT, RT and ET, respectively. These decreases were correlated with fatigability and with Type II fiber proportion (P<0.05). Resting SR measures were correlated with Type II proportion (r>=0.51, P<0.05). ET had lower resting Ca2+ release, Ca2+uptake and Ca2+ATPase (P<0.05) than UT and RT (P<0.05), probably due to their lower Type II proportion; only minor effects were found in RT. Thus SR function is markedly depressed with fatigue in controls and in athletes, is dependent upon fibre type, and appears to be minimally affected by chronic training status.




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