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J Appl Physiol (March 22, 2002). doi:10.1152/japplphysiol.00627.2001
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Articles in PresS, published online ahead of print March 22, 2002
J Appl Physiol, 10.1152/jap.00627.2001
Submitted on June 18, 2001
Accepted on March 13, 2002

The Effect of Exercise Training on the Expression of Select Adrenal Medullary Catecholamine Biosynthetic Enzymes and NPY mRNA

S. Remzi Erdem1, Haydar A Demirel2, Christopher S Broxson3, Bistra B Nankova4, Esther L Sabban4, and Nihal Tumer5*

1 Department of Pharmacology/Therapeutics, University of Florida, Gainesville, Florida, USA
2 Center for Exercise Science, University of Florida, Gainesville, Florida, USA
3 Geriatric Research and Clinical Center, Malcom Randall VA Medical Center, Gainesville, Florida, USA
4 Department of Biochemstry and Molecular Biology, New York Medical College, Valhalla, New York, USA
5 Geriatric Research and Clinical Center, Malcom Randall VA Medical Center, Gainesville, Florida, USA; Department of Pharmacology/Therapeutics, University of Florida, Gainesville, Florida, USA

* To whom correspondence should be addressed. E-mail: rerdem{at}ufl.edu.

The effect of submaximal endurance training (SET) on sympathoadrenal activity is not clear. We tested the hypothesis that SET (90 min/day, 5 days/week, 12 weeks) elevates mRNA expression of catecholamine (CA) biosynthetic enzymes, tyrosine hydroxylase (TH) and dopamine ß hydroxylase (DßH) in the adrenal medullae of adult, female Sprague-Dawley rats. SET increased TH protein level by 35%, TH activity by 62%, TH mRNA expression by 40%, and DßH mRNA expression by 67%. In addition, we examined the effect of SET on Fos-related antigens (Fras), Fra-2 immunoreactivity, and activator protein-1 (AP-1) binding activity. SET increased AP-1 binding activity by 78%, however it did not affect late Fras and Fra-2 immunoreactivity. Since the regulation of neuropeptide Y (NPY) often parallels that of CAs, we also examined the effect of SET on NPY mRNA expression. Indeed, SET elevated NPY mRNA expression as well. We conclude that 1) SET elicits a pre-translational stimulatory effect on adrenomedullary CA biosynthetic enzymes, 2) another immediate early mRNA product, rather than Fra-2, may contribute to the increase in AP-1 binding activity in response to SET, and 3) SET increases NPY mRNA expression.






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