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J Appl Physiol (September 12, 2003). doi:10.1152/japplphysiol.00585.2003
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Submitted on June 6, 2003
Accepted on September 5, 2003

Carbohydrate intake during endurance exercise increases natural killer cell responsiveness to IL-2

Brian K McFarlin1*, Michael G Flynn1, Laura K Stewart1, and Kyle L Timmerman1

1 Wastl Human Peformance Laboratory, Department of Health and Kinesiology, Purdue University, West Lafayette, Indiana, USA

* To whom correspondence should be addressed. E-mail: bkmcfarlin{at}att.net.

Introduction/purpose. The purpose of this study was to evaluate the effect of high-intensity endurance exercise and carbohydrate consumption on in vitro responsiveness of natural killer (NK) to IL-2 (2.5 U.mL-1 for 24-h). Methods. Thirteen male subjects (18-26 years old; VO2peak = 59.79±5.13 mL.kg-1.mL-1) were recruited to complete two 1-h (75-80% VO2peak) cycling trials in a random counterbalanced order: carbohydrate (CHO) and placebo (PLA). Venous blood samples were collected before (PRE), immediately (POST), 2-h (2H), and 4-h (4H) after exercise. All resting samples were taken following 15 min of seated rest. NK (CD3-/56+), activated NK (CD3-/56+/69+), helper T-cell (Th, CD3+/4+), and cytotoxic T-cell (Tc, CD3+/8+) number were measured using flow cytometry. NK cell activity (NKCA) was determined using both a 51Cr release assay (NKCA-51) and activated NK cell number (NKCA-69). Results. Immune system variables were not different between CHO & PLA; with the exception of NK cell responsiveness to IL-2, where POST (116.2%) and 4H (48.4%) was significantly greater in CHO (p<0.05). NK, Th, and Tc were significantly higher POST (40.7%, 102.7%, 82.0%) and lower at 2H (-51.9%, -53.3%, -53.2%) than PRE (time effect). 4H was not different than PRE for NK, Th, and Tc. NKCA was significantly lower 2H (NKCA-51, NKCA-69) and 4H (NKCA-69) than PRE. Conclusion. CHO consumption during exercise did not prevent disruptions in unstimulated immune system function, but it did enhance NK responsiveness to IL-2.




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