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Articles in PresS, published online ahead of print July 12, 2002
J Appl Physiol, 10.1152/jap.00585.2001
Submitted on June 6, 2001
Accepted on July 11, 2002
1 Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ont, Canada
2 Pathology and Molecular Medicine, McMaster University, Hamilton, Ont, Canada
3 Medicine, McMaster University, Hamilton, Ont, Canada
4 Kinesiology, University of Windsor, Windsor, Ont, Canada
* To whom correspondence should be addressed. E-mail: terrygra{at}uoguelph.ca.
A few qualitative investigations suggested that location of muscle glycogen (G) granules in specific sites may be associated with distinct metabolic roles. We employed a transmission electron microscopic (TEM) technique to quantify the subcellular G particle size, number and location in human Vastus lateralis biopsies of 11 resting men. The intra- and inter-observer variability for the various measures was generally less than 4% and the TEM-derived G data had a strong correlation with the G concentration determined biochemically (r2 = 0.60, p = 0.005). Granule size and number were quantified in subcellular compartments (subsarcolemmal, intra- and intermyofibrillar) in different fibre types (I and II). Subcellular location was critical: G was more densely concentrated in the subsarcolemmal than in the myofibrillar space, and the single particle volume was greater in the latter. Single particle diameter ranged from 10 to 44 
m and followed a normal distribution. There were significant differences in the subcellular distribution of G between fibre types; particles being bigger in the type II fibres, and the proportion of intra- to intermyofibrillar G was greater in the type I fibres. These results demonstrate a compartmentalised pattern of subcellular G deposition in human skeletal muscle.
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