A long-lasting fatigue was measured in human biceps muscle, following 40 maximal isokinetic concentric or eccentric contractions of the forearm, as the response to single-shock stimuli every minute for 4 hours. This protocol allowed new observations on the early time course of long-lasting fatigue. Concentric contractions induced a novel progressive decline to 30.2% (SEM 7.8, n = 7) control at 23 min with complete recovery by 120 min. Eccentric contractions lead initially to a smaller force reduction of similar time course followed by a slower decline to 40.0% (SEM 5.1, n = 7) control at 120 min with recovery less than half complete at 4 hours. 50 Hz test stimuli overcame both fatigues identifying low-frequency fatigue. EMG recordings from the biceps muscle showed moderate (<20%) changes during the fatigue. A visual tracking task showed no decrement in performance at the time of maximal fatigue of the single-shock response. As the eccentric contractions have a similar activation, a larger force but much smaller metabolic usage than concentric contractions, it is concluded that the initial decline is related to the effects of metabolites whilst the slower phase after eccentric contractions is associated with mechanical stress.