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J Appl Physiol (October 5, 2001). doi:10.1152/japplphysiol.00566.2001
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Articles in PresS, published online ahead of print October 5, 2001
J Appl Physiol, 10.1152/jap.00566.2001
Submitted on June 4, 2001
Accepted on September 29, 2001

Gender-specific K+ channel contribution to adenosine-inducedrelaxation in porcine coronary arterioles

Cristine L Heaps1* and Douglas K Bowles1

1 Biomedical Sciences, University of Missouri, Columbia, MO, USA; Dalton Cardiovascular Research Center, University of Missouri, Columbia, MO, USA

* To whom correspondence should be addressed. E-mail: heapsc{at}missouri.edu.

We examined the contribution of K+ channel activity on basal tone and adenosine-mediated relaxation of coronary arterioles isolated from sexually mature male and female miniature swine. Arterioles (~100-200 µm ID) isolated from the apical region of the heart were cannulated and studied using videodimensional analysis under constant intraluminal pressure. Coronary arterioles from male and female pigs demonstrated similar levels of basal tone and reductions in basal diameter in response to the K+ channel blockers, 4-aminopyridine (4AP; 1mM), tetraethylammonium (TEA; 1mM), and glibenclamide (Glib; 10µM), with 4AP producing significantly greater constriction than TEA or Glib. Following endothelin-induced preconstriction, relaxation responses to adenosine were not significantly different between coronary arterioles of male and female pigs. Inhibition of 4AP-sensitive channels significantly impaired adenosine-mediated relaxation in arterioles from male, but not female pigs. However, inhibition of K+ channels with iberiotoxin (100nM) or Glib had no effect on adenosine-induced relaxation in either gender. Results obtained in the presence of nitric oxide synthase inhibition suggest a potential interaction of 4AP-sensitive channels and nitric oxide at low adenosine concentrations. In conclusion, our data indicate that 4AP-sensitive channels: 1) contribute significantly to basal tone in coronary arterioles of both male and female pigs, 2) contribute to adenosine-mediated relaxation in male, but not female pigs and 3) can contribute to adenosine-induced relaxation independent of nitric oxide production in male pigs. These data are consistent with a significant role for KV channels in adenosine-mediated relaxation of coronary arterioles from males.




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