Journal of Applied Physiology Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

J Appl Physiol (November 21, 2003). doi:10.1152/japplphysiol.00460.2003
This Article
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
96/3/1069    most recent
00460.2003v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Witczak, C. A
Right arrow Articles by Sturek, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Witczak, C. A
Right arrow Articles by Sturek, M.
Submitted on May 2, 2003
Accepted on November 14, 2003

Exercise Prevents Diabetes-Induced Impairment in Superficial Buffer Barrier in Porcine Coronary Smooth Muscle

Carol A Witczak1 and Michael Sturek2*

1 Department of Medical Pharmacology & Physiology, University of Missouri, Columbia, MO, USA
2 Department of Medical Pharmacology & Physiology, University of Missouri, Columbia, MO, USA; Department of Internal Medicine, University of Missouri, Columbia, MO, USA; Center for Diabetes & Cardiovascular Health, University of Missouri, Columbia, MO, USA

* To whom correspondence should be addressed. E-mail: SturekM{at}Missouri.edu.

In healthy coronary smooth muscle cells, the superficial sarcoplasmic reticulum (SR) buffers rises in intracellular Ca2+ levels. In diabetic dyslipidemia, basal Ca2+ levels are increased, yet Ca2+ influx is decreased and SR Ca2+-uptake is increased. Exercise prevents diabetic dyslipidemia-induced increases in basal Ca2+ levels and decreases in Ca2+ influx. HYPOTHESIS: Diabetic dyslipidemia impairs Ca2+ extrusion via a decrease in superficial SR, and exercise will prevent these losses. METHODS: Male, Yucatan swine were maintained: control, hyperlipidemic, diabetic dyslipidemic, and diabetic dyslipidemic plus aerobically exercise trained. Intracellular Ca2+ levels were measured during depolarization-induced Ca2+ influx and caffeine-induced SR Ca2+ release. Na+-Ca2+ exchanger and plasmalemmal Ca2+-ATPase activity were assessed by inhibition with low extracellular Na+ and carboxyeosin, respectively. Superficial SR was quantified using the internal membrane dye, 3,3'-dihexyloxacarbocyanine iodide (DiOC6), and novel analysis techniques. RESULTS: In diabetic dyslipidemia, Ca2+ extrusion was impaired and superficial SR was decreased. Exercise prevented the diabetic dyslipidemia-induced decrease in superficial SR and restored plasmalemmal Ca2+ extrusion. CONCLUSION: Exercise attenuates the diabetic dyslipidemia-induced impairment in intracellular Ca2+ regulation.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
I. N. Bratz, G. M. Dick, J. D. Tune, J. M. Edwards, Z. P. Neeb, U. D. Dincer, and M. Sturek
Impaired capsaicin-induced relaxation of coronary arteries in a porcine model of the metabolic syndrome
Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2489 - H2496.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. A. Witczak, B. R. Wamhoff, and M. Sturek
Exercise training prevents Ca2+ dysregulation in coronary smooth muscle from diabetic dyslipidemic yucatan swine
J Appl Physiol, September 1, 2006; 101(3): 752 - 762.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
E. A. Mokelke, N. J. Dietz, D. M. Eckman, M. T. Nelson, and M. Sturek
Diabetic dyslipidemia and exercise affect coronary tone and differential regulation of conduit and microvessel K+ current
Am J Physiol Heart Circ Physiol, March 1, 2005; 288(3): H1233 - H1241.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
F. S. Korte, E. A. Mokelke, M. Sturek, and K. S. McDonald
Exercise improves impaired ventricular function and alterations of cardiac myofibrillar proteins in diabetic dyslipidemic pigs
J Appl Physiol, February 1, 2005; 98(2): 461 - 467.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1966 by the American Physiological Society.