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J Appl Physiol (July 13, 2006). doi:10.1152/japplphysiol.00438.2006
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Submitted on April 14, 2006
Accepted on July 3, 2006

Proteolytic mRNA Expression in Response to Acute Resistance Exercise in Human Single Skeletal Muscle Fibers

Yifan Yang1, Bozena Jemiolo1, and Scott W. Trappe1*

1 Human Performance Laboratory, Ball State University, Muncie, Indiana, United States

* To whom correspondence should be addressed. E-mail: strappe{at}bsu.edu.

The purpose of this study was to characterize changes in mRNA expression of select proteolytic markers in human slow (MHC I)- and fast (MHC IIa)-twitch single skeletal muscle fibers following a bout of resistance exercise (RE). Muscle biopsies were obtained from the vastus lateralis of eight young healthy sedentary males [23±2yr (mean±SD), 93±17kg, 183±6cm] before, and 4 and 24 h after 3x10 repetitions of bilateral knee extensions at 65% of 1-repetition maximum. The mRNA levels of TNF-{alpha}, calpains 1 and 2, MuRF-1, atrogin-1, caspase-3, Bcl-2 and Bax were quantified using real-time RT-PCR. Generally, MHC I fibers had higher (1.6- to 5.0-fold, P < 0.05) mRNA expression pre- and post-RE. One exception was a higher (1.6 to 3.9-fold, P < 0.05) Bax/Bcl-2 mRNA ratio in MHC IIa fibers pre- and post-RE. RE increased (1.4- to 4.8-fold, P < 0.05) MuRF-1 and caspase-3 mRNA levels 4-24 h post-RE in both fiber types, while Bax/Bcl-2 mRNA ratio increased 2.2-fold (P < 0.05) at 4 h post-RE only in MHC I fibers. These results suggest that MHC I fibers have a greater proteolytic mRNA expression pre- and post-RE compared to MHC IIa fibers. The greatest mRNA induction following RE were in MuRF-1 and Caspase-3 in both fiber types. This altered and specific proteolytic mRNA expression among slow- and fast-twitch muscle fibers indicates that the ubiquitin/proteasomal and caspase pathways may play an important role in muscle remodeling with RE.




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