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J Appl Physiol (July 26, 2007). doi:10.1152/japplphysiol.00437.2007
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Submitted on April 20, 2007
Accepted on July 4, 2007

Muscle sarcoplasmic reticulum calcium regulation in humans during consecutive days of exercise and recovery

T. A. Duhamel1, R. D. Stewart2, A. Russell Tupling2, J. Ouyang3, and H. J. Green2*

1 St.Boniface General Hospital Research Centre, University of Manitoba, Winnipeg, Canada
2 Department of Kinesiology, University of Waterloo, Waterloo, Canada
3 Department of Kinesiology, University of Waterloo, Canada

* To whom correspondence should be addressed. E-mail: green{at}healthy.uwaterloo.ca.

The study investigated the hypothesis that 3 consecutive days of prolonged cycle exercise would result in a sustained reduction in the Ca2+-cycling properties of the vastus lateralis in the absence of changes in the sarcoplasmic (endoplasmic) reticulum Ca2+-ATPase (SERCA) protein. Tissue samples were obtained at pre (Pre) and post (Post) exercise on day 1 (E1) and day 3 (E3) and during recovery days 1 (R1), 2 (R2) and 3 (R3) in 12 active but untrained volunteers (age 19.2 ± 0.27 yr; mean±SE) and analyzed for changes (nmol.mg protein-1.min-1) in maximal Ca2+-ATPase activity (Vmax), Ca2+-uptake and Ca2+-release (Phase 1 and Phase 2) and SERCA isoform expression (SERCA1a and SERCA2a). At E1, reductions (P<0.05) from Pre to Post in Vmax (150±7 vs 121±7), Ca2+-uptake (7.79±0.28 vs 5.71±0.33) and both phases of Ca2+-release (Phase 1, 20.3±1.3 vs 15.2±1.1; Phase 2, 7.70±0.60 vs 4.99±0.48) were found. In contrast to Vmax, which recovered at Pre E3 and then remained stable at Post E3 and throughout recovery, Ca2+-uptake remained depressed (P<0.05) at E3 Pre and Post and at R1 as did Phase 2 of Ca2+-release. Exercise resulted in an increase (P<0.05) in SERCA1a (14% at R2) but not SERCA2a. It is concluded that rapidly adapting mechanisms protect Vmax following the onset of regular exercise but not Ca2+-uptake and Ca2+-release.




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