Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

J Appl Physiol (September 24, 2004). doi:10.1152/japplphysiol.00437.2004
This Article
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
98/1/282    most recent
00437.2004v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yoshioka, Y.
Right arrow Articles by Kubokawa, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoshioka, Y.
Right arrow Articles by Kubokawa, M.
Submitted on April 26, 2004
Accepted on September 15, 2004

Noninvasive measurement of temperature and fractional dissociation of imidazole in human lower leg muscles using 1H-nuclear magnetic resonance spectroscopy

Yoshichika Yoshioka1*, Hiroshi Oikawa2, Shigeru Ehara2, Takashi Inoue3, Akira Ogawa3, Yoshiyuki Kanbara4, and Manabu Kubokawa1

1 Department of Physiology II, Iwate Medical University, Morioka, Japan
2 Department of Radiology, Iwate Medical University, Morioka, Japan
3 Department of Neurosurgery, School of Medicine, Iwate Medical University, Morioka, Japan
4 High Field Magnetic Resonance Imaging Research Institute, Iwate Medical University, Takizawa, Japan

* To whom correspondence should be addressed. E-mail: yyoshiok{at}iwate-med.ac.jp.

The temperature change of the fractional dissociation of imidazole ({alpha}-imidazole) in resting human lower leg muscles was measured noninvasively using 1H-NMR spectroscopy at 3.0 and 1.5 T on 5 normal male volunteers aged 30.6±10.4 (mean±SD) years. Using 1H-NMR spectroscopy, water, carnosine, and creatine in the muscles could be simultaneously analyzed. Carnosine contains imidazole protons. The chemical shifts of water and carnosine imidazole protons relative to creatine (-CH3) could be used for estimating temperatures and {alpha}-imidazole, respectively. Using the chemical shift, the values of temperature in gastrocnemius (GAS) and soleus muscles (SOL) at ambient temperature (21 to 25°C) were estimated to be 35.5±0.5 and 37.4±0.6°C (mean±SE), respectively (significantly different; P < 0.01). The estimated values of {alpha}-imidazole in these muscles were 0.620±0.007 and 0.630±0.013 (mean±SE), respectively (not significant). Alternation of the surface temperature of the lower leg from 40 to 10°C significantly changed the temperature in GAS (P < 0.0001) from 38.1±0.5 to 28.0±1.2°C, and the {alpha}-imidazole in GAS decreased from 0.631±0.003 to 0.580±0.011 (P < 0.05). However, the values of {alpha}-imidazole and the temperature in SOL were not significantly affected by this maneuver. These results indicate that the {alpha}-imidazole in GAS changed significantly with alternation in muscle temperature (r=0.877, P < 0.00001), and its change was estimated to be 0.0058/°C.




This article has been cited by other articles:


Home page
 StrokeHome page
D. Ishigaki, K. Ogasawara, Y. Yoshioka, K. Chida, M. Sasaki, S. Fujiwara, K. Aso, M. Kobayashi, K. Yoshida, K. Terasaki, et al.
Brain Temperature Measured Using Proton MR Spectroscopy Detects Cerebral Hemodynamic Impairment in Patients With Unilateral Chronic Major Cerebral Artery Steno-Occlusive Disease: Comparison With Positron Emission Tomography
Stroke, September 1, 2009; 40(9): 3012 - 3016.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
W. Derave, M. S. Ozdemir, R. C. Harris, A. Pottier, H. Reyngoudt, K. Koppo, J. A. Wise, and E. Achten
beta-Alanine supplementation augments muscle carnosine content and attenuates fatigue during repeated isokinetic contraction bouts in trained sprinters
J Appl Physiol, November 1, 2007; 103(5): 1736 - 1743.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1966 by the American Physiological Society.