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1 Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania, United States
2 Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States
3 Department of Biochemistry and Biophysics, United States; Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania, United States
* To whom correspondence should be addressed. E-mail: wilsondf{at}mail.med.upenn.edu.
Oxygen pressures, measured by oxygen dependent quenching of phosphorescence, in the intravascular (blood plasma) and interstitial (pericellular) spaces were compared. Our hypothesis was that the capillary wall does not significantly impede oxygen diffusion from the blood plasma to the pericellular space. A new oxygen sensitive probe, a Pd-tetrabenzoporphyrin encapsulated inside generation 2 poly-arylglycine (AG) dendrimer covered with oligoethylene glycol residues (Av. MW 350), was used. This sensor, Oxyphor G3, is biologically inert and was injected into thigh muscle using a 30 gage needle. Histograms of the oxygen pressure in the interstitial space in awake and anesthetized animals are compared with those for Oxyphor G2 in the intravascular (blood plasma) space. For awake mice, the lowest 10% of oxygen pressures for the interstitial and intravascular spaces (believed to represent capillary bed) were not different (23.8 ± 4.5 and 25 ± 4.3 mm Hg, respectively) whereas in isoflurane anesthetized mice there was a small but significant (p =0.01) difference (20.4 ± 6.3 and 27.9 ± 3.5 mm Hg, respectively). The peaks for the interstitial space in awake and isoflurane anesthetized mice were 40.8 ± 7.5 and 36.9 ± 8.3 mm Hg, while those for the intravascular space were 52.2 ± 4.9 and 55.9 ± 8.4 mm Hg, respectively, with no different due to anesthesia. The histograms for the intravascular space were wider, with more contribution at higher oxygen pressures. Anesthesia with intraperitoneal ip ketamine plus xylazine caused a marked decrease in tissue oxygen pressure that was variable among mice.
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