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J Appl Physiol (June 27, 2003). doi:10.1152/japplphysiol.00359.2003
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Submitted on April 10, 2003
Accepted on June 23, 2003

Toll-like receptor 4 and CD14 mRNA expression are lower in resistive exercise trained, elderly women

Michael G Flynn1*, Brian K McFarlin1, Melody D Phillips2, Laura K Stewart1, and Kyle L Timmerman1

1 Department of Health and Kinesiology, Purdue University, West Lafayette, IN, USA
2 School of Applied Health and Educational Psychology, Oklahoma State University, Stillwater, OK, USA

* To whom correspondence should be addressed. E-mail: mickflyn{at}purdue.edu.

The purpose of this study was to examine the influence of resistive exercise training and hormone status on mRNA expression of toll-like receptor 4 (TLR4), CD14, interleukin-1 {beta} (IL-1{beta}), interleukin-6 (IL-6) and tumor necrosis factor-{alpha} (TNF-{alpha}). Resistive exercise trained women on "traditional" hormone replacements (HRT, n = 9), not taking hormones (NHR, n = 6) or taking medications known to influence bone (MIB, n = 7), were compared to untrained subjects not taking supplemental hormones (CON, n = 6). Blood was taken from trained subjects before (PRE), immediately after (POST) and two hours after (2HPO) resistive exercise (same time points for resting CON). TLR4 mRNA expression (RT-PCR) was not different among groups or across time, but was significantly (p = 0.044) lower (1.9-fold) when trained groups were collapsed and compared to CON. There was also a significant group effect (p < 0.0001) for TLR4 mRNA when expressed per monocyte. CD14 expression was significantly (p = 0.006) lower (2.3-fold) for training groups collapsed and compared to CON. CD14 mRNA, expressed per monocyte, was significantly lower POST for NHR, HRT and MIB compared to CON. There were few significant effects detected for IL-6, IL-1{beta} and TNF-{alpha} mRNA, but there was a significant group effect (p < 0.0001) for TNF-{alpha} mRNA expressed per monocyte (CON > HRT, NHR, MIB). These findings suggest that there may be a resistive exercise training-induced reduction in TLR4/CD14 expression in older women. Further research is needed to determine whether lower TLR4/CD14 could explain the lower LPS-stimulated inflammatory cytokines observed in these women.




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