In single frog skeletal myocytes, a linear relationship exists between "fatigability" and oxidative capacity. The purpose of this investigation was to study the relationship between the intracellular PO₂ (P_IO₂) offset kinetics and fatigability in single Xenopus laevis myocytes in order to test the hypothesis that P_IO₂ offset kinetics would be related linearly with myocyte fatigability and, by inference, oxidative capacity. Individual myocytes (n=30) isolated from lumbrical muscle were subjected to a 2-min bout of isometric peak tetanic contractions at either 0.25 or 0.33 Hz frequency while P_IO₂ was measured continuously via phosphorescence quenching techniques. The mean response time (MRT; time to 63% of the overall response) for P_IO₂ recovery from contracting values to resting baseline was calculated. Following the initial square-wave constant frequency contraction trial, each cell performed an incremental contraction protocol (i.e., frequency increase every 2-min from 0.167, 0.25, 0.33, 0.5, 1.0, 2.0 Hz until peak tension fell below 50% of initial values [TTF]). TTF values ranged from 3.39 to 10.04 min for the myocytes. The P_IO₂ recovery MRT ranged from 26 to 146 s. A significant (p<0.05), negative relationship (MRT = -12.68TTF + 168.3, r² = 0.605) between TTF and P_IO₂ recovery MRT existed. These data demonstrate a significant correlation between fatigability and oxidative phosphorylation recovery kinetics consistent with the notion that oxidative capacity determines, in part, the speed with which skeletal muscle can recover energetically to alterations in metabolic demand.