Aims: The aim of this study was to investigate the effect and mechanism of Synchronized Gastric Electrical Stimulation (SGES) on gastric emptying in non-obese mice with diabetic gastroparesis (DB-GP). Methods: Eight control mice and forty-eight non-obese diabetic (NOD) mice with two pairs of gastric electrodes were used in this study. The study included seven groups in a randomized order (control, Diabetes (DB), DB-GP, DB+SGES, DB-GP+SGES, DB-GP+Atropine, and DB-GP+SGES+Atropine groups). In the control, DB, DB-GP group, or DB-GP+Atropine group, gastric emptying was measured in BLAB/cJ mice (control group) or NOD mice with a duration of diabetes of 0-7 days (DB group) or 28-35 days (DB-GP or DB-GP+Atropine group). In the DB+SGES, DB-GP+SGES, or DB-GP+SGES+Atropine group, the experiment was the same as the corresponding DB, DB-GP, or DB-GP+Atropine group except that SGES was applied during the experiment. SGES was applied via the proximal pair of electrodes and synchronized with the intrinsic gastric slow waves. Results: 1) Gastric emptying was delayed in NOD mice with a duration of diabetes of 28-35 days. 2) SGES was able to significantly increase gastric emptying in both diabetic mice and diabetic gastroparetic mice. 3) The excitatory effect of SGES was completely blocked by atropine. Conclusions: SGES accelerates gastric emptying in NOD mice with diabetic gastroparesis. The effect of SGES on gastric emptying is mediated via the cholinergic pathway. These findings suggest that SGES may have a therapeutic potential for treating patients with diabetic gastroparesis.