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J Appl Physiol (May 12, 2005). doi:10.1152/japplphysiol.00319.2005
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Submitted on March 21, 2005
Accepted on May 9, 2005

Elevated temperature decreases sensitivity of P2X purinergic receptors in skeletal muscle arteries

Heidi A. Kluess1, John B. Buckwalter1, Jason J. Hamann1, and Philip S. Clifford1*

1 Department of Anesthesiology and Physiology, Medical College of Wisconsin and VAMC, Milwaukee, WI, USA

* To whom correspondence should be addressed. E-mail: pcliff{at}mcw.edu.

We hypothesized that elevated temperatures would attenuate, but reduced temperatures would potentiate the tension mediated by vascular P2X receptors. Twenty-four rats were sacrificed. The femoral arteries were dissected out and placed in modified Krebs-Henseleit buffer. Arteries were cut into 2mm sections and mounted in organ tissue baths. Maximal tension (grams) was measured during a KCl and norepinephrine challenge. Tension was measured during doses of {alpha},{beta}-methyleneATP (10-7 to 10-3M), phenylephrine (10-7 to 10-4M), and acetylcholine (10-9 to 10-5M), with tissue bath temperature adjusted to 35, 37, and 41oC. Dose response curves were fit using non-linear regression analysis to calculate the EC50 and slope. The peak tension was lower with {alpha},{beta}-methylene ATP during 41oC (1.49±0.14grams) compared to 35oC (2.08±0.09grams) and 37oC (1.94±0.09grams; p<0.05). Slope and EC50 were not affected by temperature. Tension produced by phenylephrine and relaxation to acetylcholine were not affected by temperature. These data indicate that the vasoconstrictor response to {alpha},{beta}-methylene ATP is sensitive to temperature. Moderate cooling does not potentiate P2X mediated vasoconstriction, but elevated temperature attenuates the vasoconstrictor response to P2X purinergic receptors.




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