This study was designed to assess differences in the intensity of exercise to attenuate postprandial lipemia (PPL). Thirteen healthy males (age 23.8 ± 0.9 yrs) participated in three random-ordered trials: in low (25% VO₂peak - LOW) and moderate (65% VO₂peak - MOD) intensity exercise trials, which were completed one hour before a high fat meal (1.3 g fat/kg body mass), and a control (CON), fat meal only, trial. Venous blood samples were obtained before the fat meal, and at 2h, 4h, 6h, 8h and 20h after the fat meal. PPL in the MOD trial (267 ± 50 mg^.dl^-1.8h) was lower when compared to that in either CON (439 ± 81 mg^.dl^-1.8h) or LOW (403 ± 91 mg^.dl^-1.8h) trials (P < .05), while there was no difference in PPL between CON and LOW trials (P >.05). High density lipoprotein cholesterol (HDL-C) and HDL₂-C were not different between or within trials (P >.05). Postprandial insulinemia was lower in the MOD trial (20.5 ± 5.7 uIU^.ml^-1.8h; P <.05), but not in the LOW trial (31.4 ± 4.7 uIU^.ml^-1.8h), when compared to that in the CON trial (34.9 ± 5.0 uIU^.ml^-1.8h). Glucose concentration increased in all trials after the meal, and at 2h it was higher in the LOW trial (92.6 ± 1.9 mg^.dl^-1) compared to the CON trial (85.8 ± 2.0 mg^.dl^-1; P <.05). Postheparin lipoprotein lipase activity (LPLA) at 8h was higher in the LOW trial when compared to that in either CON or MOD trials, whereas there were no differences between trials at 20h. These results suggest that when exercise is performed one hour before a fat meal, only exercise of moderate but not of low intensity attenuates PPL, and that this effect is not associated with changes in postheparin LPLA.