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1 Pediatric Endocrinology, University of California, Los Angeles, Los Angeles, California, United States
2 Exercise Physiology Laboratories, University of New Mexico, Albuquerque, New Mexico, United States
3 Internal Medicine, University of New Mexico, Albuquerque, New Mexico, United States
4 Exercise Physiology Laboratories, University of New Mexico, albuquerque, New Mexico, United States; Tijeras, New Mexico, United States
5 Exercise Physiology Laboratories, University of New Mexico, albuquerque, New Mexico, United States; Dept of Exercise, NASA, Albuquerque, New Mexico, United States
* To whom correspondence should be addressed. E-mail: pyamada{at}mednet.ucla.edu.
Heat acclimation (HA) results in whole body adaptations that increase heat tolerance, however HA may also result in cellular adaptations. We hypothesized that after HA, basal intracellular (IC) Hsp 72 and extracellular (EC) interleukin (IL)-10 levels would increase while EC Hsp 72 levels decrease. 10 male and 2 female subjects completed a 10-day exercise/HA protocol (100-minute exercise bout at 56% of VO2max in a 42.5°C DB, 27.9% RH environment); subjects exhibited classic adaptations that accompany HA. Peripheral blood mononuclear cell (PMBC) were isolated before and after each acclimation session on days 1, 6 and 10; plasma and serum were collected before and after exercise on the 1st and 10th day of HA. SDS-PAGE was used to determine PBMC Hsp 72 levels during HA and ELISA was used to measure plasma IL-10 and serum Hsp 72 concentrations. The increase in PBMC Hsp 72 from pre- to post-exercise on the 1st day of HA was not significant (mean±SD, 1.0±0 vs. 1.6±0.6 density units, respectively). Pre-exercise Hsp 72 levels on day 1 were significantly lower compared to the pre- and post-exercise samples on days 6 and 10 (mean±SD, day 6: 2.1±1.0, 2.2±1.0, day 10: 2.0±1.3, 2.2±1.0 density units, respectively, p<0.05). There were no differences in IL-10 and serum Hsp 72 post-exercise or after 10 days of HA. The sustained elevation of Hsp 72 from days 6 to 10 may be evidence of a cellular adaptation to HA that may contribute to improved heat tolerance and a reduced heat illness risk.
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