To test whether there is a regional difference in the exercise pressor reflex within a given muscle, we investigated the relationship between the inflection points of cardiovascular responses and muscle oxygenation during exercise. Seven subjects performed incremental exercise, which consisted of incremental 30 s static knee extensions, each separated by 30 s of recovery. The workload started at 5% maximal voluntary contraction (MVC) and increased by 5% MVC for each increment until exhaustion. Changes in the concentrations of oxygenated Hb ([O₂Hb]) and deoxygenated Hb ([HHb]) were monitored in proximal and distal portions of the vastus lateralis muscle using near-infrared spectroscopy. The inflection points of mean arterial pressure (MAP), calf vascular resistance (CVR), and the muscle deoxygenation index ([O₂Hb - HHb]) were calculated as the intersection point of two regression equations obtained at lower and higher workloads. The inflection point of [O₂Hb - HHb] differed significantly between proximal and distal portions (28.5±3.0% vs. 39.5±3.05%MVC, P<0.05). Linear regression analysis showed significant correlations between the inflection point of [O₂Hb - HHb] in the distal portion and MAP (r=0.89; P<0.01) and CVR (r=0.89; P<0.05), but no significant relationship between the inflection point in the proximal portion and MAP or CVR. These data show that the inflection point of muscle deoxygenation differs between proximal and distal portions within the vastus lateralis muscle during incremental exercise, and suggest that the distal portion of the vastus lateralis muscle contributes more to the pressor response than does the proximal portion.