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J Appl Physiol (June 2, 2005). doi:10.1152/japplphysiol.00197.2005
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Submitted on February 17, 2005
Accepted on May 30, 2005

Effect of carbohydrate ingestion on exercise-induced alterations in metabolic gene expression

Laura J. Cluberton1, Sean L. McGee1, Robyn M. Murphy1, and Mark Hargreaves1*

1 Exercise and Nutrition Sciences, Deakin University, Burwood, VIC, Australia

* To whom correspondence should be addressed. E-mail: m.hargreaves{at}unimelb.edu.au.

Skeletal muscle possesses a high degree of plasticity and can adapt to both the physical and metabolic challenges it faces. An acute bout of exercise is sufficient to induce the expression of a variety of metabolic genes such as GLUT-4, PDK4, UCP-3 and PGC-1. Reducing muscle glycogen levels prior to exercise potentiates the effect of exercise on many genes. Similarly, altered substrate availability induces transcription of many of these genes. The purpose of this study was to determine if glucose ingestion attenuates the exercise-induced increase in a variety of exercise responsive genes. Six male subjects (28±7 yrs; 83±3 kg; VO2peak = 46±6 ml.kg-1.min-1) performed 60 min of cycling at 74±2% of VO2peak on two separate occasions. On one occasion, subjects ingested a 6% carbohydrate drink (GLU). On the other occasion, subjects ingested an equal volume of a sweet placebo (CON). Muscle samples were obtained from vastus lateralis at rest, immediately after exercise and three hours after exercise. PDK4, UCP-3, PGC-1 and GLUT-4 mRNA levels were measured on these samples using real time RT-PCR. Glucose ingestion attenuated (p<0.05) the exercise-induced increase in PDK-4 and UCP-3 mRNA. A similar trend (p=0.09) was observed for GLUT-4 mRNA. In contrast, PGC-1 mRNA increased following exercise to the same extent in both conditions. These data suggest that glucose availability can modulate the effect of exercise on metabolic gene expression.




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