The purpose of this study was to examine how inducing fatigue of the (i) lumbar erector spinae (LES) and (ii) cervical erector spinae (CES) muscles affected the ability to maintain head stability during walking. Triaxial accelerometers were attached to the head, upper trunk and lower trunk to measure accelerations in the vertical, anterior-posterior, and mediolateral directions during walking. Using three accelerometers enabled two adjacent upper body segments to be defined: the neck segment and trunk segment. A transfer function was applied to root mean square (RMS) acceleration, peak power and harmonic data derived from spectral analysis of accelerations to quantify segmental gain. The structure of upper body accelerations were examined using measures of signal regularity and smoothness. The main findings were that head stability was only affected in the AP direction, as accelerations of the head were less regular following CES fatigue. Further, following CES fatigue the central nervous system altered the attenuation properties of the trunk segment in the AP direction, presumably to enhance head stability. Following LES fatigue the trunk segment had greater gain, and increased regularity and smoothness of accelerations in the ML direction. Overall, the results of this study suggest that erector spinae fatigue differentially altered segmental attenuation during walking according to the level of the upper body that was fatigued, and the direction that oscillations were attenuated. A compensatory postural response was not only elicited in the sagittal plane where greater segmental attenuation occurred, but also in the frontal plane where greater segmental gain occurred.