Journal of Applied Physiology http://www.adinstruments.com/labchart/faseb
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

J Appl Physiol (June 21, 2007). doi:10.1152/japplphysiol.00160.2007
This Article
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
103/3/1037    most recent
00160.2007v2
00160.2007v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Perlman, C. E
Right arrow Articles by Bhattacharya, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Perlman, C. E
Right arrow Articles by Bhattacharya, J.
Submitted on February 7, 2007
Accepted on June 20, 2007

Alveolar expansion imaged by optical sectioning microscopy

Carrie E Perlman1 and Jahar Bhattacharya1*

1 Departments of Medicine and Physiology and Cellular Biophysics, College of Physicians and Surgeons and St. Luke's-Roosevelt Hospital Center, Columbia University, New York, New York, United States

* To whom correspondence should be addressed. E-mail: jb39{at}columbia.edu.

During lung expansion, the pattern of alveolar perimeter distension is likely to be an important determinant of lung functions as for example, surfactant secretion. However, the segmental characteristics of alveolar perimeter distension remain unknown. Here, we applied real-time confocal microscopy in the isolated, perfused rat lung to determine the micromechanics of alveolar perimeter distension. To image the alveolar perimeter, we loaded alveolar epithelial cells with a fluorescent dye that we microinjected into the alveolus. Then, we viewed single alveoli in a 2 µm-thick optical section at a focal plane 20 µm deep to the pleural surface at baseline. In each alveolus, we identified 5-8 segments of the perimeter. For each segment, we determined length (Lseg) by means of image analysis. At baseline alveolar pressure (Palv) of 5 cmH2O, Lseg averaged 46 µm. We hyperinflated the lung to Palv of 20 cmH2O and identified the same optical section as referenced against morphologic landmarks. Hyperinflation increased mean Lseg by 14%. However, segment distension was heterogeneous even within the single alveolus. Further, distension was greater in alveolar type 1 than type 2 epithelial cells. These findings indicate that alveoli expand non-uniformly, suggesting that segments that distend the most might be preferred alveolar locations for injury in conditions associated with lung over-distension.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. Felder, M. Siebenbrunner, T. Busch, G. Fois, P. Miklavc, P. Walther, and P. Dietl
Mechanical strain of alveolar type II cells in culture: changes in the transcellular cytokeratin network and adaptations
Am J Physiol Lung Cell Mol Physiol, November 1, 2008; 295(5): L849 - L857.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. P. Desai, K. E. Chapman, and C. M. Waters
Mechanical stretch decreases migration of alveolar epithelial cells through mechanisms involving Rac1 and Tiam1
Am J Physiol Lung Cell Mol Physiol, November 1, 2008; 295(5): L958 - L965.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
E. U. Azeloglu, J. Bhattacharya, and K. D. Costa
Atomic force microscope elastography reveals phenotypic differences in alveolar cell stiffness
J Appl Physiol, August 1, 2008; 105(2): 652 - 661.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1948 by the American Physiological Society.