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J Appl Physiol (June 18, 2004). doi:10.1152/japplphysiol.00088.2004
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Submitted on January 28, 2004
Accepted on June 15, 2004

FATIGUING EXERCISE REDUCES DNA-BINDING ACTIVITY OF NF-{kappa}B IN SKELETAL MUSCLE NUCLEI

William J Durham1, Yi-Ping Li1, Eric Gerken1, Mehran Farid1, Sandrine Arbogast2, Robert R Wolfe3, and Michael B Reid2*

1 Pulmonary Medicine, Baylor College of Medicine, Houston, TX, USA
2 Department of Physiology, University of Kentucky College of Medicine, Lexington, KY, USA
3 Department of Surgery, University of Texas Medical Branch, Galveston, TX, USA; Department of Metabolism, Shriners Burns Hospital, Galveston, TX, USA

* To whom correspondence should be addressed. E-mail: michael.reid{at}uky.edu.

This study tested the hypothesis that skeletal muscle contraction activates nuclear factor-{kappa}B (NF-{kappa}B), a putative regulator of muscle protein breakdown. Muscle biopsies were obtained the vastus lateralis of healthy humans before, immediately after, and 1h after fatiguing resistance exercise of the lower limbs. Biopsies were analyzed for nuclear NF-{kappa}B DNA-binding activity using electrophoretic mobility shift assay. NF-{kappa}B activity measured immediately after exercise was less than pre-exercise activity; after 1h recovery, activity returned to pre-exercise levels. In follow-up studies in adult mice, basal NF-{kappa}B activity varied among individual muscles. NF-{kappa}B activity in diaphragm fiber bundles was decreased after a 10-min bout of fatiguing tetanic contractions in vitro. NF-{kappa}B activity in soleus was increased by 12 days of unloading by hindlimb suspension; this increase was reversed by 10 min of fatiguing exercise. These data provide no support for our original hypothesis. Instead, acute fatiguing exercise appears to decrease NF-{kappa}B activity in muscle under a variety of conditions.




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