Local vasoconstriction plays an important role in maintaining blood pressure in spinal cord-injured individuals (SCI). We aimed to unravel local vasoconstrictor mechanisms (veno-arteriolar reflex (VAR) and myogenic response) using both limb dependency and cuff inflation in SCI and compare these with control subjects. Limb blood flow was measured in 11 male SCI (age: 24-55 year) and 9 male controls (age: 23-56 year) using venous occlusion plethysmography in forearm and calf during three levels of (1) limb dependency, and (2) cuff inflation. During limb dependency vasoconstriction relies on both the VAR and the myogenic response. During cuff inflation the decrease in blood flow is caused by the VAR and by a decrease in arterio-venous pressure difference, whereas the myogenic response does not play a role. At the highest level of leg dependency the percent increase in calf vascular resistance (mean arterial pressure/calf blood flow) was more pronounced in SCI than in controls (SCI: 186±53% and, controls: 51±17% (P=0.032)). In contrast, during cuff inflation no differences were found between SCI and controls (SCI: 17±17% and, controls: 14±10%). Percent changes in forearm vascular resistance in response to either forearm dependency or forearm cuff inflation were equal in both groups. Thus, local vasoconstriction during dependency of the paralyzed leg in SCI is enhanced. The contribution of the VAR to local vasoconstriction does not differ between the groups, since no differences between groups existed for cuff inflation. Therefore, the augmented local vasoconstriction in SCI during leg dependency relies, most likely, on the myogenic response.