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J Appl Physiol 97: 2200-2206, 2004. First published September 3, 2004; doi:10.1152/japplphysiol.00970.2003
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Mucociliary and long-term particle clearance in the airways of healthy nonsmoker subjects

Winfried Möller,1 Karl Häußinger,2 Renate Winkler-Heil,3 Willi Stahlhofen,1 Thomas Meyer,4 Werner Hofmann,3 and Joachim Heyder1

1Clinical Research group "Inflammatory Lung Diseases" of the GSF, National Research Centre for Environment and Health, Institute for Inhalation Biology, and 2Asklepios Clinic for Respiratory Medicine, 4Intelligent Aerosol Medicine, Inamed, D-82131 Gauting, Germany; and 3Institute of Physics and Biophysics, University of Salzburg, Salzburg, Austria

Submitted 8 September 2003 ; accepted in final form 16 August 2004


   ABSTRACT
TOP
 ABSTRACT
METHODS
 RESULTS
 DISCUSSION
 GRANTS
 ACKNOWLEDGMENTS
 REFERENCES
 
Spherical monodisperse ferromagnetic iron oxide particles of 1.9-µm geometric and 4.2-µm aerodynamic diameter were inhaled by 13 healthy nonsmoking subjects using the shallow bolus technique. The bolus width was 100 ml, and the penetration front depth was 150 ± 27 ml. The mean flow rate during inhalation and exhalation was 250 ml/s. The Fowler dead space and the phase 1 dead space of the airways were 282 ± 49 and 164 ± 34 ml, respectively. Deposition was below 20% without breath holding and 51 ± 8% after an 8-s breath-holding time. We attempted to confine the bolus deposition to the bronchial airways by limiting the bolus front depth to the phase 1 dead space volume. Particle retention was measured by the magnetopneumographic method over a period of 9 mo. Particle clearance from the airways showed a fast and a slow phase; 49 ± 9% followed the fast phase with a mean half-time of 3.0 ± 1.6 h and characterized the mucociliary clearance. The remaining fraction was cleared slowly with a half-time of 109 ± 78 days. The slow clearance phase was comparable to clearance measurements from the lung periphery of healthy nonsmokers, which allowed macrophage-dependent clearance mechanisms of the slow cleared fraction to be taken into account. Despite the fact that part of the slowly cleared particles may originate from peripheral deposition, the data demonstrate that mucociliary clearance does not remove all particles deposited in the airways and that a significant fraction undergoes long-term retention mechanisms, the origin of which is still under discussion.

airways; mucociliary clearance; long-term retention; bolus inhalation

DEPOSITION AND CLEARANCE of inhaled environmental particles are highly correlated to the different anatomic structures in the human lung (29). During inhalation, the lung acts as a serial filter system and removes particles from the inhaled air. Large particles are deposited effectively in the extrathoracic region (nose, larynx) and intrathoracic airway bifurcations due to impaction. Inertia causes larger particles not to follow the airstream at bifurcations and deposit effectively on the airway epithelium. This mechanism is effective for high inhalation flow rates. On penetration into the deeper parts of the lung, the air velocity decreases rapidly, and a second mechanism, sedimentation, causes the particles to fall out of the airstream and deposit on the wall of small airways and in the alveoli. The two thoracic regions differing in their main deposition mechanism also differ in their mechanism of clearance. The airways are covered by a mucus layer that is transported out of the lung by ciliary beating, resulting in fast removal of deposited particles. In the lung periphery, no mucus transport is present. Alveolar macrophages are the defense cells in this region. They phagocytose particles, bacteria, or viruses and digest them. Particles of low solubility can be retained for long times within the lungs and are digested within macrophages.

Human studies using the bolus inhalation technique have shown that mucociliary clearance (MC) removes all deposited particles larger than 6 µm from the airways within 24 h. When smaller particles are deposited, a certain fraction is retained for longer than 24 h (42, 45, 46). This fraction increases with decreasing particle size. The mechanisms of this long-term clearance of particles from the airways are unclear. Different hypotheses have been developed to describe this phenomenon: 1) a fraction of particles (depending on size and surface properties) may penetrate through the mucus and between cilia (20, 21, 23, 43), 2) the mucus is not a continuous layer but has discontinuous patches and holes where the particles will directly deposit in the periciliary fluid on the airway epithelium (31, 33, 34), and 3) a fraction of particles may penetrate deeper into the lung and reach alveolar structures due to different mechanisms in lung ventilation, like parabolic lung filling, asymmetric and asynchronous filling, or cardiogenic mixing (17, 44). These particles follow long-term clearance mechanisms. Airway infections and ciliary dysfunctions (primary ciliary dyskinesia) can lead to impaired mucus transport (10, 11) and can thereby enhance the fraction of slowly cleared particles.

In humans, there are no experimental data available about the kinetics of slowly cleared particles from the airways beyond an observation time of some weeks (which results from the radiolabeling technique). The purpose of this study was to evaluate both the mucociliary and the long-term clearance kinetics of inhaled magnetic iron oxide particles from the airways of healthy nonsmokers using magnetopneumography (MPG) (12, 47). The magnetic label can be detected as long as the iron ions remain in the specific ferrimagnetic crystal structure. Dissolved ions do not contribute to the signal, even if they are metabolized in the lung. The advantage of the magnetic labeling technique compared with radioactive labeling is that it has no physical decay constant and allows a long observation period of up to 1 yr and the prevention of artifacts in clearance measurements due to leaching of the radiolabel and metabolization of the dissolved material in organs. This method has been applied to investigate long-term clearance from the alveoli (36), which result in clearance half-times of ~120 days for healthy nonsmokers and impairment of clearance due to cigarette smoking and interstitial lung diseases, e.g., idiopathic pulmonary fibrosis and sarcoidosis.

Studies of airway clearance require a deposition of the test particles predominantly in the airways. Efforts were made to achieve this requirement by controlled inhalation of particle boli at the end of tidal inhalation (8, 41). The phase 1 volume of the anatomic dead space was used as a threshold volume for the bolus penetration depth.


   METHODS
TOP
 ABSTRACT
 METHODS
RESULTS
 DISCUSSION
 GRANTS
 ACKNOWLEDGMENTS
 REFERENCES
 
Subjects and pulmonary function testing.   Thirteen healthy subjects (age of 37 ± 11 yr), who had never smoked, participated in the study. Anamnestic data were collected using a questionnaire based on American Thoracic Society (ATS) recommendations (16), and all subjects were interviewed by a pulmonary specialist. None of the subjects reported a history of seasonal allergic rhinitis or the cardinal symptoms of airway hyperreactivity (wheezing, chest tightness, shortness of breath). None of the healthy subjects had a history of respiratory or cardiovascular disease or was receiving any long-term medication. The protocol was approved by the Ethical Committee of the Medical School of the Ludwig Maximilian University (Munich, Germany), and informed consent from each subject was obtained. Body plethysmography and spirometry were performed using a Jäger Masterlab (Erich Jäger, Würzburg, Germany). Predicted values of conventional lung function parameters were calculated by normalizing to the reference values proposed by the European Community for Steel and Coal (39). A lung function test and a MPG measurement of the natural ferromagnetic contamination of the lungs of every subject were obtained before inhalation. MPG measurements were performed 30 min, 3 and 6 h, 1 and 2 days, 1 wk, and 1, 3, 6, and 9 mo after particle inhalation.

Volumetric dead space measurement.   To measure the physiological dead space, a fast mass spectrometer (modified magnetic sector field mass spectrometer; DLT 1100 R, Dennis Leigh Technology, Sandbach, UK) was used, which allowed the measurement of the concentrations of various respiratory and nonrespiratory gases within the respired air as a function of the respired air volume. To measure the physiological dead space, a tracer gas mixture of 0.2% C18O2, 21% O2, and 78.8% N2 was applied as a single-breath inhalation. The subjects were positioned in front of the inhalation device. Starting from functional residual capacity (FRC), subjects inhaled a volume of the tracer gas and then exhaled to a lung volume below the FRC. This breathing maneuver was performed three times at a constant air flow, controlled by the subject using a visual flow signal. Figure 1A shows a typical expiration diagram of C18O2 together with the estimated volumes. The physiological dead space was derived from the C18O2 expirogram using the method of Fowler (19) and Meyer et al. (35). CO2 labeled with the stable oxygen isotope 18O (C18O2) was completely taken up in the gas-exchanging region of the lung but not from the airways. Therefore, C18O2 was only expired from the dead space of the lung and not from the alveolar region. Hence, C18O2 allows the measurement of the respiratory dead space not only in healthy subjects but also in patients with chronic obstructive pulmonary disease and lung emphysema. As has been previously shown, the physiological dead space measured by this technique showed a high correlation with the dead space measured conventionally with nitrogen. The dead space volume obtained by the protocol of Fowler in part includes alveolar structures. In addition, a closer threshold volume for the conducting airways was estimated from the gas-expiration profile, where we analyzed the volume in which the C18O2 concentration dropped to below the 95% level (see Fig. 1A). This volume was called the phase 1 dead space volume (VDP1) and characterized the beginning of the diffusive process of the tracer gas into the periphery of the lung. In all subjects, the dead space volumes were determined at an end-inspiratory volume of 90% total lung capacity (TLC). The reason for the 90% TLC lung expansion was to have one protocol for healthy subjects and for patients (not included in this report). For example, chronic obstructive pulmonary disease patients with emphysema have a higher FRC due to trapped air.



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  		Fig. 1. A: illustration of the measurement of the airway dead space volume by inhalation of test gas consisting of the C18O2 tracer. The graph shows the exhalation profile of the gas and the estimation of the Fowler dead space (VDF) and the phase 1 dead space (VDP1) (threshold of 5% concentration drop). B: aerosol concentration of the inhaled shallow bolus shortly before the end of inhalation, and the exhaled bolus after 8 s of breath holding. Arrows indicate VDF and VDP1 in this subject. The dotted line shows the C18O2 expirogram.

 
Magnetic particle generation, inhalation, and MPG detection.   Around 0.5–1 mg of spherical monodisperse ferrimagnetic iron oxide particles (Fe3O4; 4.2 µm aerodynamic, 1.9 µm geometric diameter, {sigma}g < 1.1) were deposited in the lungs by controlled voluntary inhalation. The particles were produced by a spinning top aerosol generator (37). A colloidal water solution of nonmagnetic iron oxide (Fe2O3) was nebulized into uniform droplets in an N2 atmosphere. After evaporation of the water, the colloidal subunits aggregated and formed compact spherical particles. With the use of a virtual impactor, the aerosol was concentrated 10- to 15-fold, and the flow rate was reduced from 40 to 1 l/min. After the concentration stage, H2 gas was added to a final concentration of 0.5%. The concentrated aerosol was fed through a furnace at 800°C, where the nonmagnetic iron oxide (hematite, Fe2O3) was reduced to the magnetic form of iron oxide, magnetite (Fe3O4). The particles were fed through a second virtual impactor, which reduced the aerosol flow from 2 to 0.2 l/min, resulting in a fivefold aerosol concentration.

The output of the second concentrator fed into a vertical tube, which acted as an aerosol storage tank. This tank was coupled to the aerosol input channel of the respiratory aerosol probe (8). The respiratory aerosol probe consisted of a computer-controlled valve system, which can switch between clean air and aerosol during inhalation. The flow rate and aerosol concentration were continuously measured during in- and exhalation. The mean inhalation and exhalation flow rates were kept at 250 ml/s. The aerosol was administered as a 100-ml bolus at the end of inhalation to a defined volumetric front depth (VF). At the end of inhalation, an 8-s breath hold was performed to enhance the particle deposition. The end-inspiratory volume was 1 liter above the FRC. The lung expansion of the dead space measurements was 90% TLC and, therefore, larger compared with that of the aerosol administration. Therefore, the volumetric dead space during aerosol administration requires a 10% reduction, as can be estimated from the data in Bennett et al. (6), where the dead space volume was measured at different levels of lung expansion. About 20–30 breath were necessary to deposit 0.5–1 mg of magnetite particles in the lung.

Directly after inhalation, the particles were detected by the MPG system (47). The subject lay on a bed with the lungs directly under the magnetizing coils (magnet). Magnetization was carried out by discharging a capacitor battery (1 mF, 1,000 V) into a copper coil (40 cm mean diameter), which produced a magnetic field pulse of 100 mT for 30 ms. The magnetized particles formed remanent magnetic dipoles, oriented parallel to the magnetizing field. After the magnetizing current decayed, the magnetized particles produced a weak remanent magnetic field of the lung of ~50–100 pT. The subject was moved under a superconducting loop array, where the weak magnetic field of the lungs was detected by a superconducting quantum interference device. The entire system was enclosed in a magnetically shielded room. After natural ferromagnetic contamination was corrected for, the remanent magnetic field detected was shown to be a reliable measure of the amount of particles retained within the lungs (38). Subjects were studied over a 8- to 9-mo postinhalation period.

Data analysis.   Particles deposited in the lung by the shallow bolus technique showed at least two different mechanisms of clearance. The first fast phase happened within the first day, and later proceeded into the slow phase of clearance. The course of the clearance curve was fitted by the sum of two exponential functions according to:

where B0 describes the amount or retained magnetic material directly after inhalation, 1 – AS describes the amount of fast-cleared material with the time constant TF, AS describes the amount of slowly cleared material, and TS is the time constant of the slowly cleared material. Other authors suggest more than two clearance phases and therefore use a multiterm exponential function, but our data do not suggest more than the two proposed mechanisms. Incorporating an additional intermediate clearance phase results only in a small fraction with less statistical significance. Additionally, the amount of retained material after 24 h was analyzed.


   RESULTS
TOP
 ABSTRACT
 METHODS
 RESULTS
DISCUSSION
 GRANTS
 ACKNOWLEDGMENTS
 REFERENCES
 
Data of pulmonary function testing, anatomic dead space, and particle inhalation.   Lung function data of the study groups are shown in Table 1. The lung function data of all subjects are within the band of healthy subjects. None of the subjects had a history of cigarette smoking or gave rise to any chronic inflammatory processes within the lungs. Figure 1A shows a typical washout profile of the test gas C18O2 together with the estimation of the Fowler dead space volume (VDF) and VDP1 (threshold volume of 95% C18O2 concentration). The mean values for VDF and VDP1 at a lung inflation of 90% TLC are shown in Table 2 and are 282 ± 49 and 164 ± 34 ml, respectively. In the healthy subjects, VDF and VDP1 show a high correlation (coefficient of correlation, r = 0.91, P < 0.001). The aerosol bolus was administered at the end of a 1-liter breath from FRC, in which the mean lung expansion was 70 ± 7% in the healthy nonsmokers. To adapt the lung inflation of the dead space measurements to the aerosol inhalation, a reduction of the dead space volumes of ~10–15% is necessary, according to data in Ref. 6 and our few measurements. Figure 1B shows a typical concentration profile of an inhaled and an exhaled aerosol bolus in one subject. The breath holding time of 8 s between inhalation and exhalation is not shown. The bolus penetration (front depth), as shown in Fig. 1B in relation to the Fowler dead space and the phase 1 dead space, is 59% of VDF and 100% of VDP1 as corrected to 70% TLC lung expansion. The mean volume of aerosol penetration front depth during bolus inhalation was VF = 150 ± 27 ml, where the mean deposition was 51 ± 8% after 8 s of breath-holding time. The deposition without breath hold was below 20%. The bolus penetration depth VF and VDP1 are correlated (r = 0.82, P < 0.01).


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  		Table 1. Age and lung function data of healthy subjects

 

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  		Table 2. Results of measurement of airway dead space volumes using C18O2 tracer gas method of particle properties and inhalation parameters, and of the clearance curve parameters

 
The aerodynamic particle size was measured using a sedimentation cell, and particle concentration was measured using a laser aerosol spectrometer. The particle size distribution obtained by sedimentation cell measurements revealed a geometric standard deviation of {sigma}g < 1.1; therefore, the particles can be characterized as monodisperse. The particles were very compact (density {rho} = 4.9 g/cm3), were chemically stable, and resisted dissolution in physiological saline, body fluids, and the lungs for several months.

Fast clearance of particles from the airways.   The retention of the ferromagnetic iron oxide particles was measured in the MPG system directly after, and 3 h, 6 h, 1 day, 2 days, 1 mo, 3 mo, 6 mo, and 9 mo after inhalation. The mean course of the retention of all healthy nonsmokers within the first 24 h is shown in Fig. 2. The data follow a two-phase decay with a fast phase within the first day and a slow phase over the following months. The mean data of the half-times of the two-phase decay and the fraction of clearance after the slow decay (AS) are given in Table 2. After 24 h, 49 ± 8% of the particles were retained in the lung. Extrapolating the long-term decay back to time 0 reveals that 50 ± 8% of the particles follow the slow phase of retention. Only 50% of the particles depend on the mucociliary fast clearance mechanism, which happens with a half-time of 3.0 ± 1.6 h.



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  		Fig. 2. Retention of 2-µm-diameter ferromagnetic iron oxide particles in the airways of 13 healthy nonsmokers within the first 24 h. Data show mean values (thick curve) ± SD (thin curve).

 
Slow clearance of particles from the airways.   The slow phase of airway clearance of 1.9-µm geometric diameter iron oxide particles is shown in Fig. 3. Within the first day, 50% of the particles were cleared via the mucociliary apparatus, and the remaining particles followed a mean clearance half time of 109 ± 78 days (270 days of measurement time). After 9 mo, 10 ± 9.8% of the initially deposited particles were retained in the lungs. Attempts to include an intermediate clearance phase into the model failed. Only ~10% of the long-term-retained particles might follow a clearance mechanism with a half time of 15 days, but the level of statistical significance debased.



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  		Fig. 3. Long-term retention of 2-µm-diameter ferromagnetic iron oxide particles in the airways of 13 healthy nonsmokers within 250 days postinhalation. Data show mean values (thick curve) ± SD (thin curve).

 

   DISCUSSION
TOP
 ABSTRACT
 METHODS
 RESULTS
 DISCUSSION
GRANTS
 ACKNOWLEDGMENTS
 REFERENCES
 
Many open questions arise from the bronchial clearance measurements after shallow bolus inhalation compared with the recent understanding of MC. The fact that not all particles are removed from the bronchial tree within 24 h can have several reasons. Despite the possibility that a fraction of particles may reach alveolar structures, even with the use of the bolus technique, the remaining part may get lost from the mucociliary escalator. Our studies do not provide data on how this may happen. A complex interaction between ventilation, deposition, and mucus properties and transport may be involved. The mechanism underlying the long-term clearance phase cannot be identified. However, compared with other histological studies, we can address airway macrophages as being possible target cells in the long-term clearance mechanisms.

Long-term airway clearance.   Further studies have shown that, after shallow bolus inhalation, a fraction of particles is long-term retained within the conducting airways of the human lung and that the fraction of long-term-retained particles depends primarily on the geometric particle size (46, 48). The fraction of long-term-retained particles decreases with increasing particle size and vanishes for particles larger than 6 µm. Part of this particle size-dependent clearance may be caused by decreasing deposition fraction of larger particles in the periphery. All of these studies were performed using radiolabeled particles of different chemical composition and different density, which were detected in the lung by a sensitive lung counter. The results of this study using magnetic particles in combination with the MPG detection technique coincide with the above data, where 2-µm particles result in ~50% long-term particle retention in the airways. Therefore, effects due to the labeling technique can be excluded.

It has been suggested that the main reason for the long-term retention was the penetration of particles into peripheral (alveolar) structures (17, 44), where the particles are subject to the macrophage-mediated clearance, which has a half-time of several months for iron oxide particles (36). The low deposition of only 50% after 8-s breath hold in this study (below 20% without breath hold) suggests that the inhaled bolus primarily stays in bronchial structures with diameters in the millimeter range. The settling distance of 4.2-µm aerodynamic diameter particles is 4.2 mm in 8 s. Inhaling the aerosol into tubes with a smaller width will collect most particles and cause a high deposition, even in a system of randomly oriented tubes. In addition, the mean bolus penetration front depth (VF = 150.0 ± 26.9 ml) coincides with the mean VDP1, which suggests that the bolus primarily remains in the airways, followed by a subsequent particle deposition in this region. The possibility of transporting the particles deeper into the lung, for example due to asynchronous filling or cardiogenic mixing, must also happen during normal breathing and, therefore, should be incorporated into the dead space measurements using the C18O2 method, because gas reaching alveolar structures is absorbed and not exhaled. Therefore, VDP1 can be assumed as a safe threshold volume for the gas and particle penetration into the airways.

Further studies have shown that the fraction of slowly cleared particles was constant for as long as the bolus remained in the conducting airways (48). A small bolus with a width of 30 ml was used, and the front depth of this bolus was varied between 40 and 180 ml. The fraction of slowly cleared particles increased for boluses penetrating deeper than 200 ml, which accounts for increasing alveolar deposition of the particles. This also suggests that, as long as the particles do not penetrate deeper than the phase 1 dead space, we get particle deposition primarily in the conducting airways. These results in humans are, to some degree, in contradiction to clearance measurements in beagle dogs after shallow bolus inhalation (32), which show that the fraction of long-term-retained particles increase with increasing penetration depth. This may be due to different anatomic structures and different mechanisms of lung ventilation in the beagle dog compared with the human lung. Nevertheless, the clearance studies in beagle dogs after shallow bolus inhalation demonstrate the long-term clearance in the airways but predict a fraction of ~50% of the long-term-retained particles originating from alveolar deposition. Additionally, it was shown in humans that the fraction of slowly cleared particles did not depend on either inhalation flow rate or on breath-holding time (48).

Bolus inhalation studies using radioactive labeled particles and gamma camera imaging showed a left to right lung asymmetry in lung deposition using the shallow bolus technique (5, 6), which depended on the lung volume (%TLC of end-inspiratory lung inflation) but not on the anatomic dead space. The reason for this inhomogeneity may be an inhomogeneous particle deposition (i.e., due to heartbeat) or inhomogeneous ventilation. The effect may cause deviations from bulk particle transport with the possibility of a deeper penetration into smaller airways. On the other hand, both studies demonstrated a 24-h retention, which was independent of the left to right deposition asymmetry, of the lung inflation and of the anatomic dead space, suggesting that the effect may not have a strong impact on the regional particle distribution and the slow clearance mechanisms.

The detection of long-term-retained particles in the airways may imply a loss of particles from the mucociliary transport machinery and a transport of deposited particles to the submucus space. Morphometric studies revealed that the particle surface properties and the interaction with surfactant seem to play a key role (20, 43). After deposition, the particles are coated with surfactant and then get displaced into the aqueous subphase, where they may be submerged and penetrate between the cilia. Additionally, it was shown that the mucus fluid does not form a continuous layer (25, 26). Particle deposition in such holes allows direct contact with beating cilia. With decreasing particle size, the probability increases that particles can penetrate between cilia or between cilia supporting cells, where they can easily be engulfed by macrophages.

Mechanisms of long-term airway clearance.   In each subject, the long-term clearance was recorded over a 270-day period. Figure 4 summarizes the mean long-term retention curve of this study compared with the clearance of 1.3-µm geometric diameter (2.9-µm aerodynamic diameter) iron oxide particles from the alveolar region of healthy nonsmokers and smokers (36). The mean age of the subjects, who participated in the alveolar clearance study, was 30 ± 4 yr, and the cigarette consumption of the smokers was 11.7 ± 10 pack-years (1 pack-year corresponds to the smoking of one pack of cigarettes per day over a period of 1 yr). The alveolar clearance study showed a small fraction of fast-cleared particles (5% in nonsmokers and 11% in smokers), which accounts for a small a fraction of bronchial particle deposition. The alveolar long-term clearance kinetics revealed a mean half-time of 124 ± 66 days in nonsmokers and 208 ± 82 days in smokers. In nonsmokers, the half-times of the bronchial and the alveolar clearance of iron oxide particles are comparable, and the logarithmic plot in Fig. 4 shows a parallel slope. Despite the possibility that a small fraction of the long-term clearance after bolus inhalation may reflect alveolar clearance, we can suggest that the long-term clearance underlies comparable mechanisms in the bronchial and in the alveolar region. Because alveolar clearance is primarily a function of alveolar macrophages, we can suggest that the long-term particle retention in the airways may be a function of bronchial macrophages.



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  		Fig. 4. Comparison of the mean long-term retention of iron oxide micro-particles from the airways of healthy nonsmokers (AW, NS) with retention from the alveolar region in healthy nonsmokers (AL, NS) and smokers (AL, S). 20–39, Age group of the subjects involved in the alveolar clearance study.

 
Further studies show that a separate population of macrophages can be found in the airways (7), which have specific characteristics that distinguish them from alveolar macrophages (1, 24). Histological and stereological studies have revealed that, already 20 min after inhalation of Latex or Teflon particles, a certain fraction can be found in airway macrophages (22, 30), and 24 h after particle inhalation >80% of the remaining particles are phagocytized by airway macrophages. The present study together with the histological results suggests that long-term particle retention in the airways may be a function of airway macrophages.

Studies by Alexis et al. (2) have shown that a fraction of the radiolabeled particles being deposited by the aerosol bolus technique can be removed from the lung by induced sputum. During the first hours, sputum induction significantly enhanced the clearance of particles, whereas after 24 h there was no effect of induced sputum on clearance, and ~40% of the particles were long-term retained within the lungs.

MC studies compared with the bolus technique.   MC in humans was intensively studied in the past, and pathophysiological conditions and pharmacological interventions were investigated (4, 9, 18, 27, 28, 40). All of these studies use radiolabeled aerosols (mostly tagged with 99mTc). The observation time is limited to the decay time of the radiotracer. Most of these studies detect the clearance of the radiotracer from the lung by gamma camera imaging in a hospital; therefore, most studies have observation times of not more than 2 h. This is not sufficient to characterize the kinetics of MC (half-time of ~3 h), and it is impossible to characterize its effectiveness. At least 24 h of detection time is required for a characterization of airway clearance. Studies that fulfill this criterion show a significant particle retention after 24 h, which might indicate either peripheral deposition or long-term airway clearance (2, 13). There are few studies that detected airway clearance over longer time periods (up to 6 mo) using different radiotracers and very sensitive lung counters (14, 15). These studies use a specific slow inhalation technique in combination with large particles (6-µm aerodynamic diameter) to deposit particles primarily in small airways. The data recording in these studies started at 24 h past inhalation, and therefore no data about MC were presented. In the bronchial clearance study by Svartengren et al. (49), the slow inhalation technique and the bolus technique were directly compared and showed only small differences in the 24-h retention, although both techniques are presumed to have different main sites of particle deposition. By modeling considerations, it has been suggested that the slow inhalation technique has the highest deposition probability in the small airways, whereas the shallow bolus technique primarily deposits particles in the larger airways.

Many of the studies using radiotracers need corrections because of a leaching of the radiotracer. Because the kinetics of 99mTc in the human body are very complex [<50% of soluble 99mTc is excreted via urine within 24 h (3)], these corrections bear some uncertainty. Additionally, in those studies, the site of particle deposition is not very well controlled. One attempt is to inhale large particles with high flow rates, which should primarily induce deposition on bifurcations due to impaction. This approach may not deposit particles homogeneously in the airways but on sites that may have less density of cilia and reduced mucus transport. Another attempt is to inhale larger particles at slow inhalation flow, which allows the particles to pass the larynx and the airway bifurcations and to fall out in the small airways. This is a strong attempt to better control the site of deposition and to reduce extrathoracic deposition.

In conclusion, with the use of the shallow bolus technique, it has been shown that clearance of particles from the conducting airways shows two distinct phases. MC does not eliminate all particles within the first 24 h after particle deposition. Depending on the particle size, a fraction of particles (50% for 2-µm-diameter particles) are retained in the long term and follow clearance mechanisms that are comparable to those in the alveolar compartment. Although a certain fraction of the long-term-retained particles may originate from particle deposition in the lung periphery, the data suggest that part of the long-term clearance mechanism is a function of airway macrophages. The data require a new interpretation of the mechanisms of particle clearance in the airways and a new assessment of particle dosimetry. Pathophysiological and pharmacological influences on the long-term particle retention in the airways (24-h retention and long-term clearance kinetics) were not yet investigated. Because macrophage-mediated clearance mechanisms play an important role in the lung periphery, cigarette smoking, lung diseases, and drugs that modulate alveolar clearance may also be of relevance in the airways and have to be investigated in the future.


   GRANTS
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 ABSTRACT
 METHODS
 RESULTS
 DISCUSSION
 GRANTS
ACKNOWLEDGMENTS
 REFERENCES
 
This study was supported by the Commission of the European Community under FIGD-CT-2000-00053.


   ACKNOWLEDGMENTS
TOP
 ABSTRACT
 METHODS
 RESULTS
 DISCUSSION
 GRANTS
 ACKNOWLEDGMENTS
REFERENCES
 
We thank Christiane Herpich and Martina Schulte for performing lung function and dead space measurements.


   FOOTNOTES
 

Address for reprint requests and other correspondence: W. Möller, GSF—National Research Centre for Environment and Health, Institute for Inhalation Biology, Robert Koch Allee 29, D-82131 Gauting, Germany (E-mail: moeller{at}gsf.de)

The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.


   REFERENCES
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 ABSTRACT
 METHODS
 RESULTS
 DISCUSSION
 GRANTS
 ACKNOWLEDGMENTS
 REFERENCES
 

  1. Alexis N, Soukup J, Niekens S, and Becker S. Phenotype and function of airway macrophages are associated with markers of asthma severity and airways inflammation. J Allergy Clin Immunol 105: S296–S296, 2000.
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