Journal of Applied Physiology Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 95: 742-750, 2003. First published May 2, 2003; doi:10.1152/japplphysiol.00109.2003
8750-7587/03 $5.00
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
95/2/742    most recent
00109.2003v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wu, Z.-X.
Right arrow Articles by Dey, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, Z.-X.
Right arrow Articles by Dey, R. D.

Substance P released from intrinsic airway neurons contributes to ozone-enhanced airway hyperresponsiveness in ferret trachea

Zhong-Xin Wu, Brian E. Satterfield, and Richard D. Dey

Department of Neurobiology and Anatomy, Robert C. Byrd Health Sciences Center, West Virginia University, Morgantown, West Virginia 26506

Submitted 3 February 2003 ; accepted in final form 29 April 2003


   ABSTRACT
TOP
 ABSTRACT
METHODS
 RESULTS
 DISCUSSION
 DISCLOSURES
 ACKNOWLEDGMENTS
 REFERENCES
 
Exposure to ozone (O3) induces airway hyperresponsiveness mediated partly through the release of substance P (SP) from nerve terminals in the airway wall. Although substantial evidence suggests that SP is released by sensory nerves, SP is also present in neurons of airway ganglia. The purpose of this study was to investigate the role of intrinsic airway neurons in O3-enhanced airway responsiveness in ferret trachea. To remove the effects of sensory innervation, segments of ferret trachea were maintained in culture conditions for 24 h before in vitro exposure to 2 parts/million of O3 or air for 1 h. Sensory nerve depletion was confirmed by showing that capsaicin did not affect tracheal smooth muscle responsiveness to cholinergic agonist or contractility responses to electrical field stimulation (EFS). Contractions of isolated tracheal smooth muscle to EFS were significantly increased after in vitro O3 exposure, but the constrictor response to cholinergic agonist was not altered. Pretreatment with CP-99994, an antagonist of the neurokinin 1 receptor, attenuated the increased contraction to EFS after O3 exposure but had no effect in the air exposure group. The number of SP-positive neurons in longitudinal trunk ganglia, the extent of SP innervation to superficial muscular plexus nerve cell bodies, and SP nerve fiber density in tracheal smooth muscle all increased significantly after O3 exposure. The results show that release of SP from intrinsic airway neurons contributes to O3-enhanced tracheal smooth muscle responsiveness by facilitating acetylcholine release from cholinergic nerve terminals.

airway smooth muscle; tachykinins; neurokinin receptors

OZONE (O3), a chemical irritant associated with air pollution, has been shown to increase specific airway resistance in human subjects (20) and induce airway hyperresponsiveness (AHR) and neutrophilic inflammation in a variety of animal models, including rats, dogs, and guinea pigs (23, 25, 26, 38). Substance P (SP) levels in human airway lavage fluid are increased after O3 exposures (18), and several studies demonstrate that O3 activates bronchial C fibers, which store and release SP (9, 43). SP released from excitatory nonadrenergic noncholinergic sensory nerves has been linked to airway hyperresponsiveness (39). Early studies demonstrated that SP produces smooth muscle constriction and increases vascular permeability in guinea pig airway (29). Although SP has a direct effector action on smooth muscle contractility and permeability in some species, it has no direct effect on tracheal smooth muscle responsiveness in ferret trachea, and all neurally mediated contraction in ferret trachea is cholinergic (47). However, SP also acts as a neuromodulator increasing cholinergic sensitivity of airway smooth muscle (6) and increasing the release of acetylcholine (ACh) from cholinergic neurons in the airways (36), either of which could affect airway smooth muscle responsiveness.

Although SP is generally considered a sensory neuropeptide in the airways, it is also synthesized in the parasympathetic neurons of intrinsic airway ganglia of the ferret trachea (11, 13). However, the possibility that airway neurons release SP during O3 exposure and their contribution to AHR has not been examined. Our laboratory reported previously that in vivo O3 exposure enhanced airway responsiveness to cholinergic agonists and to electrical field stimulation (EFS) and that a neurokinin (NK) 1-receptor antagonist partially abolished this response (47). The response persisted throughout a culture period intended to deplete the airway of extrinsic, sensory innervation. The findings suggested that O3-induced AHR is partially mediated through SP released from neurons in airway ganglia. However, these experiments did not entirely eliminate the possibility that the hyperresponsiveness observed after culture may have resulted from the persistent effect of SP released by sensory nerve fibers that were viable during the exposure period or in the early hours of the culture period. To address this problem, we developed a method for in vitro O3 exposure. This approach would allow O3 exposure of tracheal segments that had been cultured for 24 h, a procedure that depletes airways of extrinsic innervation, including sensory nerves (15, 49). AHR was assessed by measuring tracheal smooth muscle responses to methacholine (MCh), which reflects tracheal smooth muscle sensitivity to cholinergic agonist, and to EFS, which evaluates tracheal smooth muscle responses resulting from the release of ACh from airway cholinergic nerves. The purpose of this study was to determine the role of SP in mediating response to in vitro O3 exposure after depletion of sensory nerves enhances smooth muscle responsiveness in ferret trachea. We hypothesize that O3 exposure increases the SP levels in parasympathetic neurons of intrinsic tracheal ganglia and that enhanced SP release increases smooth muscle contractility either directly, by increasing cholinergic contractility of tracheal smooth muscle, or indirectly, by enhancing ACh release from cholinergic nerve terminals innervating tracheal smooth muscle.


   METHODS
TOP
 ABSTRACT
 METHODS
RESULTS
 DISCUSSION
 DISCLOSURES
 ACKNOWLEDGMENTS
 REFERENCES
 
Female, nonalbino ferrets (Marshall Farms, North Rose, NY), weighing 250–500 g, were housed two to four per cage with access to food and water ad libitum in an American Association for Acceditation of Laboratory Animal Care-accredited facility. All procedures were performed in accordance with the recommendations of the Guide for the Care and Use of Laboratory Animals, published by the National Institutes of Health, and were also approved by the West Virginia University Animal Care and Use Committee.

Materials. ACh chloride, MCh chloride, atropine sulfate, hydrocortisone hemisuccinate, amphoterican B, and recrystalized bovine insulin were obtained from Sigma Chemical (St. Louis, MO). Penicillin G, streptomycin, fetal calf serum, and CMRL 1066 were obtained from GIBCO (Grand Island, NY). CP-99994 was obtained from Pfizer (Groton, CT). SP antibody was obtained from Pensinula (Belmont, CA). Fluorescein isothiocyanate-labeled goat anti-rabbit antibody was obtained from ICN Immunobiologicals (Costa Mesa, CA).

Organotypic cultures of ferret trachea. Organotypic cultures of tracheas from normal ferrets were used following a modification of our laboratory's previously described technique (15). Under sterile conditions, tracheas were removed and washed with cold culture medium (described below). The tissue was then placed in a petri dish with culture medium and cut into 60-mm-long segments beginning at the carina. After a second wash, the segments were placed directly on the bottom of petri dishes containing fresh culture medium. In some experiments, capsaicin (10-5 M), which depletes SP from sensory nerve terminals (16, 30, 45), or the vehicles of capsaicin, were added to the culture medium and maintained throughout the experiment to determine the role of SP in airway neurons. The culture medium consisted of CMRL 1066 containing 0.1 µg/ml hydrocortisone hemisuccinate, 1 µg/ml recrystalized bovine insulin, 60 µg/ml penicillin G (100 units/ml), 10 µg/ml amphotericin B, 100 µg/ml streptomycin, and 5% heat-inactivated fetal calf serum. The petri dishes were then placed in a controlled-atmosphere culture chamber and gassed with 95% O2-5% CO2. The chamber was placed on a rocker and incubated at 37°C for 24 h. After culture, smooth muscle responses were measured in the segments.

In vitro O3 exposure. The tracheal segments were mounted vertically in Krebs solution, securely tied to upper and lower hose connectors, and stretched to normal resting tension in a glass exposure vessel. The upper hose connector was connected to the mixing chamber, which is a Plexiglas cylinder used to mix the incoming humidified air and O3 streams before they are administered to the tracheal lumen. O3 was produced by an O3 generator (ENMET, Blairsville, PA) that utilizes a low-pressure mercury vapor lamp with high-output ultraviolet radiation of 254-nm wavelength producing up to 150 parts/million (ppm) of O3 at 1 l/min (0.294 mg l) with ambient air as the input gas. To avoid decomposition of O3, all the tubes exposed to O3 were made of glass or Teflon. Humidified air was generated and regulated by a humidifier (Electro-Tech Systems, Glenside, PA), a relative humidity controller (Electro-Tech System), and peristaltic pump (Barnant, Barrington, IL), which work in conjunction to maintain a set range of percent relative humidity as well as a constant downstream pressure to prevent backflow of O3 into the humidifier assembly. The relative humidity controller was set at 79%. The peristaltic pump speed was set to allow mixing of humidified air into the mixing chamber, resulting in an O3 concentration of 2 ppm. The lower hose connector was connected to an O3 analyzer (model OA 350-2R, Forney, Carrollton, TX) that continuously measured the O3 concentration in the tracheal lumen.

All in vitro O3 exposures were done at 2 ppm for 1 h. A separate group of tracheas was subjected to air exposure in which procedures identical to those described above were followed, except that O3 was not delivered to the mixing chamber.

Measurement of smooth muscle contraction in vitro. Tracheal smooth muscle reactivity was evaluated by measuring contractile responses to MCh or EFS. MCh responses measure smooth muscle responses to the applied agonist, whereas EFS evaluates tracheal smooth muscle responses resulting from the release of ACh from airway nerves. The segments from air- or O3-exposed tracheas were cut into 3-mm-wide strips, mounted in holders, and maintained in gassed (95% O2-5% CO2) modified Krebs-Henseleit (MKH) solution at 37°C with a composition (in mM) of 113 NaCl 113, 4.8 KCl, 2.5 CaCl, 1.2 MgSO4, 24 NaHCO3, 1.2 KH2PO4, and 5.7 glucose, pH 7.4. The strips were tied at each end with 4-0 silk and positioned between the rings of platinum electrodes attached to tissue holders. Each holder was anchored in a 10-ml water-jacketed organ bath, and the top string was attached to a force-displacement transducer connected to a recorder (Gould Instruments, Valley View, OH). Strips were equilibrated for 60 min at a resting tension of 1.0 g, determined in preliminary studies to be optimal for contraction, during which time the MKH solution in the baths was changed every 15 min. After equilibration, cumulative concentration-response curves for MCh were constructed for separate strips by adding a series of concentrations of MCh to the bath in half-log-increment concentrations ranging from 10-9 to 10-3 M. The next concentration was not added until the previous response reached a plateau. When the response curves were completed, strips were washed by changing the MKH solution in the baths every 10 min. About 1 h after concentration-response curves were completed and resting tension was back to baseline, EFS-induced responses were obtained with a Grass S48 stimulator (Grass Instruments, West Warwick, RI). Frequency-response curves were constructed by increasing the frequency from 1 to 30 Hz by using a submaximum voltage of 120 V, 0.2-ms pulse duration, and 10-s train duration. Between each stimulation period, 10 min were allowed for the previous response to return to baseline. EFS-induced contractions were normalized as a percentage of the response to 10-3 M ACh (%ACh response). In some experiments, atropine (10-6 M) was added to the Krebs solution to verify that the responses elicited by EFS were mediated exclusively by the release of ACh from cholinergic nerves.

Immunocytochemistry. Immunocytochemical procedures for localizing neuropeptides in neurons and nerve fibers are identical to those described previously (11, 12). Briefly, the airways were removed 1 h after the end of the O3 or air exposure, immediately fixed in picric acid-formaldehyde fixative for 3 h, and rinsed three times with a 0.1 M phosphate-buffered saline containing 0.3% Triton X-100. Then the airways were frozen in isopentane, cooled with liquid nitrogen, and stored in airtight bags at -80°C. The tracheas were frozen on cock supports and oriented with the dorsal surface uppermost so that the tracheal muscle would be sectioned in a coronal plane. Cryostat sections (12-µm thickness) were collected on gelatin-coated coverslips and dried briefly at room temperature, covered with rabbit anti-SP antibody diluted 1:200, incubated in a humid chamber at 37°C for 30 min, and rinsed three times with a solution of 1% bovine serum albumin-phosphate-buffered saline and Triton X-100, with 5 min allowed for each rinse. The sections were then covered with fluorescein isothiocyanate-labeled goat anti-rabbit antibody diluted 1:100, incubated at 37°C for 30 min, and rinsed. After all immunocytochemical procedures were conducted, the coverslips were mounted with Fluoromount and observed with a fluorescence microscope equipped with fluorescein (excitation wavelengths from 455 to 500 nm, and emission wavelengths >510 nm). Controls consisted of testing the specificity of primary antiserum by absorption with 1 µg/ml of the specific antigen. Nonspecific background labeling was determined by omission of primary antiserum.

To measure fluorescence intensity in longitudinal trunk (LT) neurons, images were digitally recorded by using an AX 70 microscope (Olympus America, Melville, NY) with the SPOT 2 digital camera (Diagnostics Instruments, Sterling Heights, MI). Fluorescence intensity of SP was measured by using commercial image-processing software (Optimas 6.5, Media Cybernetics, Silver Spring, MD). The intensity recordings were calibrated by using the InSpeck Green (505/515) microscope image intensity calibration kit (Molecular Probes, Eugene, OR). The LT neurons were identified by drawing the perimeter of the cell, and the fluorescence intensity was reported as gray level for each neuron. Neurons with a gray level <50 were considered negative because they were at or below the general background. Fluorescence intensities of >=50 were counted as labeled neurons. To measure SP innervation of superficial muscular plexus (SMP) neurons, all identifiable vasoactive intestinal peptide (VIP)-positive neurons [which has been shown to label >90% of all these neurons (11)] were subjectively scored as either innervated or not innervated on the basis of the occurrence of SP in varicosities in apparent direct contact with cell bodies. All identifiable LT and SMP neurons were evaluated in every fifth section collected from serial sections, usually amounting to a total of 10–15 sections analyzed.

For measuring nerve fiber density in tracheal smooth muscle, images of SP-containing nerve fibers were collected in series by using the Zeiss LSM 510 confocal microscope. A series of images representing all of the tracheal smooth muscle in a section were collected in digital files, saved to an internal database, and measured with Optimas software. Regions of smooth muscle were selected by using the rhodamine channel to avoid possible bias created by the presence or absence of nerve fibers. The smooth muscle regions were outlined to measure total cross-sectional area of smooth muscle. The microscope was then switched to reveal nerve fibers in the fluorescein channel and the image digitally captured. The proportion of nerve fibers was determined by segmentation using threshold gray levels with the Optimas software. Then, the percentage of nerve fiber density was calculated as the percentage of total cross-sectional area of smooth muscle occupied by SP-immunoreactive nerve fibers. At least 10 measurements were made for each section, and 15 sections were measured in each animal.

Data analysis. Unless otherwise stated, results are expressed as means ± SE. Contractions elicited by EFS were expressed as a percentage of the maximal contraction elicited by MCh. Contractions to MCh were normalized as percentage of the respective maximal responses for each agonist. The half-maximum concentration (EC50) for MCh was calculated by using a four-parameter logistic curve fit (Sigmoidal, SigmaPlot 2000). The 95% confidence interval was also calculated. Force development was expressed by normalizing force (g) divided by the wet weight of the tissue. LT neurons were expressed as a percentage of SP-positive cell bodies, and SMP neurons were expressed as a percentage of SP-innervated cell bodies. Nerve fiber density was expressed as a percentage of the area of SP-immunoreactive nerve fibers in the total area of the smooth muscle. Statistical analysis of immunocytochemisty, EC50, and EFS was performed by using one- or two-way repeated-measures ANOVA. When the main effect was considered significant at P < 0.05, pairwise comparisons were made with a post hoc analysis (Fisher's least significant difference). A value of P <= 0.05 was considered significant, and n represents the number of animals studied.


   RESULTS
TOP
 ABSTRACT
 METHODS
 RESULTS
DISCUSSION
 DISCLOSURES
 ACKNOWLEDGMENTS
 REFERENCES
 
Study 1: Effect of in vitro O3 exposure on airway responsiveness in noncultured and cultured tracheas. The initial experiments examined the effect of in vitro O3 exposure in noncultured tracheas. The cumulative dose-response curve for Mch was markedly shifted to the left after exposure to O3 (Fig. 1A), and the EC50 value for Mch (Table 1) was decreased by 68% in O3-exposed tracheal segments. Exposure to O3 also increased the smooth muscle response to EFS. A left-ward shift in the frequency-response curve to EFS was observed after O3 exposure (Fig. 1B), and contractions produced by EFS at 10 and 30 Hz were significantly increased by 33 and 26%, respectively, after O3 exposure (P <= 0.05).



View larger version (16K):
[in this window]
[in a new window]
  		Fig. 1. Cumulative concentration-response curves for methacholine (MCh; A) and frequency-response curves for electrical field stimulation (EFS; B) in noncultured tracheal smooth muscle after in vitro exposure to air ({circ}) or ozone ({bullet}). Responses to MCh are plotted as a percentage of the maximum response. Responses to EFS are plotted as a percentage of the maximum response to acetylcholine. {blacktriangleup}, Airway responses to same challenge after administration of 10-6 M atropine. Values are means ± SE; n = 6. The difference in MCh cumulative concentration-response curves between air and ozone exposure are presented in Table 1. *Significant difference in EFS between air and ozone exposure, P <= 0.05.

 

View this table:
[in this window]
[in a new window]
  		Table 1. Effect of O3 on cumulative concentration-response curves for MCh in noncultured tracheal smooth muscle

 

The next studies were done to examine the contribution of intrinsic neurons by measuring the O3-enhanced AHR of cultured tracheal segments. Previous studies have shown that innervation of smooth muscle by airway neurons remains intact during short-term culture and that SP-containing sensory neurons mostly degenerate (15, 49). Therefore, tracheal segments were maintained in organotypic culture for 24 h and then exposed to 2 ppm O3 for 1 h in the in vitro exposure chamber. The cumulative concentration-response curve for MCh (Fig. 2A) was shifted to the left and EC50 value (Table 2) for MCh was decreased by 66% in tracheal strips after O3 exposure. Contractions produced by EFS at 10 and 30 Hz were significantly increased by 29 and 30%, respectively, in tracheal strips after O3 exposure (Fig. 2B). The contractions to MCh and EFS in both noncultured and cultured tracheal segments were totally abolished after treatment with 10-6 M atropine (Figs. 1 and 2).



View larger version (15K):
[in this window]
[in a new window]
  		Fig. 2. Cumulative concentration-response curves for MCh (A) and frequency-response curves for EFS (B) in organotypic cultured tracheal smooth muscle after in vitro exposure to air ({circ}) or ozone ({bullet}). {blacktriangleup}, Airway responses to same challenge after administration of 10-6 M atropine. Values are means ± SE; n = 6. The difference in MCh cumulative concentration-response curves between air and ozone exposure are presented in Table 2. *Significant difference in EFS between air and ozone exposure, P <= 0.05.

 

View this table:
[in this window]
[in a new window]
  		Table 2. Effect of O3 on cumulative concentration-response curves for MCh in organotypic cultured tracheal smooth muscle

 

Previous studies have indicated that pretreatment with a high concentration of capsaicin depletes SP in sensory neurons (16, 49). The next studies were done to confirm that O3-exposed cultured tracheal segments lacked SP-containing sensory nerves. The rationale was that application of 10-5 M capsaicin should not affect O3-enhanced smooth muscle responses if the segments were initially depleted of sensory nerves. Even in the presence of capsaicin, the cumulative concentration-response curve for MCh (Fig. 3A) was shifted to the left and the EC50 value (Table 3) for MCh was decreased by 61% after O3 exposure. Contractions produced by EFS at 10 and 30 Hz were significantly increased by 36 and 33%, respectively, after O3 exposure and were unaltered by capsaicin (Fig. 3B). These findings support our assumption that SP-containing nerve fibers originating from sensory neurons are depleted after culture.



View larger version (16K):
[in this window]
[in a new window]
  		Fig. 3. Cumulative concentration-response curves for MCh (A) and frequency-response curves for EFS (B) in tracheal smooth muscle cultured with capsaicin after in vitro exposure to air ({circ}) or ozone ({bullet}). Values are means ± SE; n = 5. The difference in MCh cumulative concentration-response curves between air and ozone exposure are presented in Table 3. *Significant difference in EFS between air and ozone exposure, P <= 0.05.

 

View this table:
[in this window]
[in a new window]
  		Table 3. Effect of capsaicin on cumulative concentration-response curves for MCh in organotypic cultured tracheal smooth muscle after in vitro O3 exposure

 

Study 2: Effects of in vitro O3 exposure on SP release from in airway neurons. The next experiments examined the role of SP in O3-enhanced airway responsiveness by blocking the NK1 receptor. Cumulative concentration-response curve for MCh and the EFS-stimulated contractions at 10 and 30 Hz demonstrated expected changes in cultured tracheal segments after O3 exposure in the group pretreated with saline (Fig. 4, A and C, and Table 4). MCh contractions after O3 exposure were still significantly increased subsequent to the NK1 antagonist (Fig. 4B and Table 4). However, the O3-enhanced contractile responses to EFS were not different from air-exposed controls in tracheas treated with the NK1 antagonist prior to O3 exposure (Fig. 4D). EFS-stimulated airway contractions at 10 and 30 Hz increased only by 10 and 7%, respectively, after O3 in tracheas pretreated with CP-99994, compared with 27 and 33% in tracheas pretreated with saline (Table 4).



View larger version (26K):
[in this window]
[in a new window]
  		Fig. 4. Effects of saline (A and C) or CP-99994 (B and D) on cumulative concentration-response curves for MCh (A and B) and frequency-response curves for EFS (C and D) in organotypic cultured tracheal smooth muscle after in vitro exposure to air ({circ}) or ozone ({bullet}). Values are means ± SE; n = 5. The difference in MCh cumulative concentration-response curves between air and ozone exposure are presented in Table 4. *Significant difference in EFS between air and ozone exposure, P <= 0.05.

 

View this table:
[in this window]
[in a new window]
  		Table 4. Effect of CP-99994 on cumulative concentration-response curves for MCh in organotypic cultured tracheal smooth muscle after in vitro O3 exposure

 

Study 3: Changes in immunoreactive SP-containing neurons of intrinsic airway ganglia. These studies examined the effect of O3 on SP-containing cell bodies in LT and SP innervation in cell bodies of the SMP in cultured trachea. Characterization of LT and SMP ganglia has been described previously (11). About 36% of the LT cell bodies labeled for SP (Figs. 5A and 6A). After exposure to O3, nearly 56% of the cell bodies in the LT contained SP (Figs. 5B and 6A). In the SMP, ~23% of the neurons were innervated by SP-containing nerve fibers in control tracheas (Figs. 5C and 6B) but nearly 46% were innervated by SP-containing nerve fibers after O3 exposure (Figs. 5D and 6B). SP nerve fiber density from in the tracheal smooth muscle was significantly increased from 0.33% in controls to 0.47% after exposure to O3 (Figs. 5, E and F, and 6C). This represents a 45% increase in nerve fiber density.



View larger version (114K):
[in this window]
[in a new window]
  		Fig. 5. Fluorescence photomicrographs of substance P (SP)-immunoreactive nerve cell bodies and fibers in longitudinal trunk (LT; A and B) and superficial muscular plexus (SMP; C and D) and SP-immunoreactive nerve fiber density (NFD) in tracheal smooth muscle (E and F) after in vitro exposure to air (control) or ozone. A: negative SP-immunoreactive LT neurons are seen in the control ganglia. B: most of the LT neurons contain SP immunoreactivity after ozone exposure. C: few SP-immunoreactive cell bodies are present in the SMP of control. D: SP-immunoreactive cell bodies in the SMP are increased after ozone exposure. E: few SP-immunoreactive nerve fibers are present in tracheal smooth muscle of control (NFD of this micrograph is 0.36). F: increased SP-immunoreactive nerve fibers in tracheal smooth muscle after ozone exposure (NFD of this micrograph is 0.53). Magnification: x285.

 


View larger version (9K):
[in this window]
[in a new window]
  		Fig. 6. Effects of air (open bars) and ozone (solid bars) exposure on SP-containing nerve cell bodies in LT (A), SP innervation of airway neurons in SMP (B), and SP-immunoreactive (IR) NFD in tracheal smooth muscle (C). Values are means ± SE; n = 6. *Significant difference between air and ozone exposure, P <= 0.05.

 


   DISCUSSION
TOP
 ABSTRACT
 METHODS
 RESULTS
 DISCUSSION
DISCLOSURES
 ACKNOWLEDGMENTS
 REFERENCES
 
This study shows that in vitro O3 exposure enhances tracheal smooth muscle responsiveness in the ferret, as evidenced by elevated contractility to MCh and EFS. O3-induced AHR was elicited even in tracheal segments cultured for 24 h, a procedure shown to cause a significant anatomic and functional loss of SP-containing sensory fibers while maintaining viability of intrinsic airway neurons (15, 49). The efficacy of the depletion was further verified in the present experiments by showing that the O3-induced responses were not altered by application of capsaicin in cultured tracheal segments. These experiments support the conclusion that the O3-enhanced tracheal smooth muscle responsiveness did not result from sensory nerve fibers that may have survived after culture. Instead, the findings suggest that parasympathetic neurons of intrinsic airway ganglia contribute to the increased smooth muscle responsiveness induced by in vitro O3 exposure. The elevation of airway smooth muscle responses by in vitro O3 exposure was attenuated by treatment with a NK1-receptor antagonist, indicating that SP release played a key role in the mechanism responsible for the enhancement of smooth muscle contractile responses. The finding that blocking NK1 receptors reduced EFS-induced atropine-sensitive contractions but did not alter MCh sensitivity suggests that the SP released during O3 exposure increases ACh release from cholinergic nerve terminals. The observations that O3 exposure increased the proportion of neurons in the LT expressing SP, the SP innervation of SMP neurons, and the SP innervation of tracheal smooth muscle in cultured trachea all support the conclusion that O3 exposure elevates endogenous SP levels in parasympathetic neurons of airway ganglia.

SP localized in the peripheral endings of nerves innervating the lung and airways originates in nerve cell bodies located both in sensory (12, 21) and intrinsic airway (10, 11, 13, 15, 27) ganglia. Stimulation of sensory nerve afferents by inhalation of irritants is known to trigger the release of neuropeptides from afferent endings (28, 31, 46, 48). However, much of the evidence implicating SP as a mediator of altered airway responsiveness does not differentiate between sensory and intrinsic airway neurons as the source of SP. The finding that the NK1-receptor antagonist CP-99994 significantly attenuates the effect of O3 on EFS-stimulated contractile responses in cultured trachea implicates the involvement of SP as the mediator of O3 action on airway smooth muscle. Although SP is a known bronchoconstrictor in guinea pig airway (3, 29), direct action of SP on smooth muscle does not appear to be an important effect in the ferret trachea because all of the smooth muscle contractile effects of O3 were atropine sensitive. Thus the logical explanation of the O3 effect in ferret is that O3 alters tracheal smooth muscle responsiveness to EFS by increasing the production and release of SP from intrinsic tracheal neurons. Previous studies have shown that SP enhances tracheal smooth muscle responsiveness by enhancing ACh release from parasympathetic nerve terminals (34, 41, 44).

A possible alternative mechanism of SP action requires an analysis of the complex circuitry of the intrinsic airway neurons. The intrinsic airway neurons act as signal filters, limiting electrical transmission of signals between presynaptic and postsynaptic neurons (4, 32, 33), and may be involved in the integration and control of airway function (1, 7, 11). Nerve cell bodies are located in large ganglia of the LT and in ganglia of the SMP (2). LT neurons are predominantly cholinergic and cell bodies in the SMP contain predominantly vasoactive intestinal peptide (VIP) and nitric oxide (NO), with a small population containing SP (13, 14). We have demonstrated in previous studies that LT neurons project to and appear to synapse with SMP neurons (50). In control ferrets, most of the LT neurons produce ACh, and it would be assumed that the projections to the SMP ganglia release ACh, potentially activating the inhibitory nonadrenergic noncholinergic (iNANC) neurons to release bronchodilator transmitters NO and VIP. This arrangement may serve to balance the constrictor actions of ACh at airway ganglia. However, after O3 exposure, the levels of SP production in LT neurons increases. This increase correlates with an increase in innervation at the SMP neurons and supports the possibility that SP release at SMP neurons may also be increased by O3 exposure. The effect of increased SP release at SMP neurons may serve to downregulate the iNANC neurons, reducing VIP and NO release at smooth muscle. Such an effect would be consistent with enhanced tracheal smooth muscle contraction and AHR. A few studies have associated the attenuation of iNANC innervation with asthma or increased smooth muscle responsiveness. In humans, VIP nerve fiber density is reduced in individuals with severe asthma (37). Physiological studies have demonstrated reduced iNANC activity in allergen-sensitized and -challenged rabbits (17) and in young ferrets after viral infection (8). Although the effects of SP on iNANC neurons is not known, activation of NK3 receptors in guinea pig airway neurons increases excitability and facilitates synaptic transmission in cholinergic neurons (5, 35).

A role for inflammatory mediators should also be considered. Our laboratory's previous study showed increased innervation of SMP after interleukin-1{beta} treatment (49). The parallels with the findings in the present paper may suggest that the effects of O3 are mediated through the generation of inflammatory mediators. Thus the initiation of an inflammatory process by any irritant may eventually converge on a common pathway that involves upregulation of SP levels of airway neurons projecting to and modulating both airway smooth muscle and other neurons in the airway neuronal plexus.

Plasticity of intrinsic airway neurons is certainly not the only mechanism responsible for O3-induced responses and smooth muscle hyperresponsiveness. Many studies have demonstrated the important role of C-fiber activation of central vagal reflexes in mediating rapid shallow breathing, tachypnea, and bronchoconstriction after O3 exposure (40) as well as causing smooth muscle hyperresponsiveness (26). In addition to generating central reflexes, C-fiber activation initiates local axon reflexes within the airway wall, resulting in the release of SP from collateral afferent nerve fibers near smooth muscle to produce smooth muscle hyperresponsiveness (22, 25). The release of local inflammatory mediators, such as arachidonic acid metabolites (19, 24) and cytokines (42, 49), may also increase airway smooth muscle responsiveness to O3 exposure. Our findings identify the intrinsic airway neurons as another regulatory mechanism in what appears to be a highly redundant system designed to protect the distal airways and gas-exchange regions of the lung from inhaled irritants, including O3. However, because these neurons are know to project to cholinergic neurons (50), the unique role of intrinsic neurons may be to provide prejunctional modulation of ACh release from cholinergic nerve endings innervating airway smooth muscle.

In conclusion, our results show that in vitro O3 exposure increases SP levels in nerve cell bodies and fibers of intrinsic airway nerves innervating tracheal smooth muscle. At the same time, smooth muscle responses are increased in tracheal segments depleted of sensory innervation. Administration of CP-99994, an antagonist of the NK1 receptor, attenuates the in vitro O3 exposure-enhanced tracheal smooth muscle responses to EFS, indicating the enhanced responses is dependent on SP release from intrinsic nerves. These findings suggest that O3 exposure increases SP production and release from airway neurons. SP release may contribute to O3-enhanced smooth muscle responsiveness in ferret trachea by facilitating prejunctional ACh release from cholinergic nerve endings.


   DISCLOSURES
TOP
 ABSTRACT
 METHODS
 RESULTS
 DISCUSSION
 DISCLOSURES
ACKNOWLEDGMENTS
 REFERENCES
 
This study was supported by National Heart, Lung, and Blood Institute Grant RO1 HL-35812.


   ACKNOWLEDGMENTS
TOP
 ABSTRACT
 METHODS
 RESULTS
 DISCUSSION
 DISCLOSURES
 ACKNOWLEDGMENTS
REFERENCES
 
The authors are grateful to Dr. G. Hobbs (Dept. of Statistics, West Virginia University) for statistical analysis. The authors also thank Pfizer (Groton, CT) for the supply of CP-99994.


   FOOTNOTES
 

Address for reprint requests and other correspondence: R. D. Dey, Dept. of Neurobiology and Anatomy, P.O. Box 9128, West Virginia University, Morgantown, WV 26506 (E-mail: rdey{at}hsc.wvu.edu).

Original submission in response to a special call for papers on "Airway Hyperresponsiveness: From Molecules to Bedside."

The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.


   REFERENCES
TOP
 ABSTRACT
 METHODS
 RESULTS
 DISCUSSION
 DISCLOSURES
 ACKNOWLEDGMENTS
 REFERENCES
 

  1. Baker DG, Basbausm CB, Herbert DA, and Mitchell RA. Transmission in airway ganglia of ferrets: inhibition by norepinephrine. Neurosci Lett 41: 139-143, 1983.[ISI][Medline]
  2. Baker DG, McDonald DM, Basbaum CB, and Mitchell RA. The architecture of nerves and ganglia of the ferret trachea as revealed by acetylcholinesterase histochemistry. J Comp Neurol 246: 513-526, 1986.[ISI][Medline]
  3. Barnes PJ, Baraniuk JN, and Belvisi MG. Neuropeptides in the respiratory tract: part I. Am Rev Respir Dis 144: 1187-1198, 1991.[ISI][Medline]
  4. Cameron AR and Coburn RF. Electrical and anatomic characteristics of cells of ferret paratracheal ganglion. Am J Physiol Cell Physiol 246: C450-C458, 1984.[Abstract/Free Full Text]
  5. Canning BJ, Reynolds SM, Anukwu LU, Kajekar R, and Myers AC. Endogenous neurokinins facilitate synaptic transmission in guinea pig airway parasympathetic ganglia. Am J Physiol Regul Integr Comp Physiol 283: R320-R330, 2002.[Abstract/Free Full Text]
  6. Cheung D, Van Der Veen H, Den Hartigh J, Dijkman JH, and Sterk PJ. Effects of inhaled substance P on airway responsiveness to methacholine in asthmatic subjects in vivo. J Appl Physiol 77: 1325-1332, 1994.[Abstract/Free Full Text]
  7. Coburn RF. The anatomy of the ferret paratracheal parasympathetic nerve-ganglion plexus. Exp Lung Res 7: 1-9, 1984.[ISI][Medline]
  8. Colasurdo GN, Hemming VG, Prince GA, Gelfand AS, Loader JE, and Larsen GL. Human respiratory syncytial virus produces prolonged alterations of neural control in airways of developing ferrets. Am J Respir Crit Care Med 157: 1506-1511, 1998.[ISI][Medline]
  9. Coleridge JCG, Coleridge HM, Schelegle ES, and Green JF. Acute inhalation of ozone stimulates bronchial C-fibers and rapidly adapting receptors in dogs. J Appl Physiol 74: 2345-2352, 1993.[Abstract/Free Full Text]
  10. Dey RD, Altemus JB, and Michalkiewicz M. Distribution of vasoactive intestinal peptide- and substance P-containing nerves originating from neurons of airway ganglia in cat bronchi. J Comp Neurol 304: 330-340, 1991.[ISI][Medline]
  11. Dey RD, Altemus JB, Rodd AB, Mayer B, Said SI, and Coburn RF. Neurochemical characterization of intrinsic neurons in ferret tracheal plexus. Am J Respir Cell Mol Biol 14: 207-216, 1996.[Abstract]
  12. Dey RD, Altemus JB, Zervos I, and Hoffpauir J. Origin and colocalization of CGRP- and SP-reactive nerves in cat airway epithelium. J Appl Physiol 68: 770-778, 1990.[Abstract/Free Full Text]
  13. Dey RD, Hoffpauir J, and Said SI. Co-localization of vasoactive intestinal peptide- and substance P-containing nerves in cat bronchi. Neuroscience 24: 275-281, 1988.[ISI][Medline]
  14. Dey RD, Mayer B, and Said SI. Colocalization of vasoactive intestinal peptide and nitric oxide synthase in neurons of the ferret trachea. Neuroscience 54: 839-843, 1993.[ISI][Medline]
  15. Dey RD, Satterfield B, and Altemus JB. Innervation of tracheal epithelium and smooth muscle by neurons in airway ganglia. Anat Rec 254: 166-172, 1999.[Medline]
  16. Ellis JL and Undem BJ. Non-adrenergic, non-cholinergic contractions in the electrically field stimulated guinea-pig trachea. Br J Pharmacol 101: 875-880, 1990.[ISI]
  17. Fame TM, Colasurdo GN, Loader JE, Graves JP, and Larsen GL. Decrease in the airways' nonadrenergic noncholinergic inhibitory system in allergen sensitized rabbits. Pediatr Pulmonol 17: 296-303, 1994.[ISI][Medline]
  18. Hazbun ME, Hamilton R, Holian A, and Eschenbacher WL. Ozone-induced increased in substance P and 8-epi-prostaglandin F2{alpha} in the airways of human subjects. Am J Respir Cell Mol Biol 9: 568-572, 1993.[ISI][Medline]
  19. Ho CY, Gu Q, Hong JL, and Lee LY. Prostaglandin E(2) enhances chemical and mechanical sensitivities of pulmonary C fibers in the rat. Am J Respir Crit Care Med 162: 528-533, 2000.[Abstract/Free Full Text]
  20. Holtzman MJ, Cunningham JH, Sheller JR, Irsigler GB, Nadel JA, and Boushey HA. Effects of ozone on bronchial reactivity in atopic and nonatopic subjects. Am Rev Respir Dis 120: 1059-1067, 1979.[ISI][Medline]
  21. Hunter DD and Undem BJ. Identification and substance P content of vagal afferent neurons innervating the epithelium of the guinea pig trachea. Am J Respir Crit Care Med 159: 1943-1948, 1999.[Abstract/Free Full Text]
  22. Joad JP, Kott KS, and Bonham AC. Exposing guinea pigs to ozone for 1 wk enhances responsiveness of rapidly adapting receptors. J Appl Physiol 84: 1190-1197, 1998.[Abstract/Free Full Text]
  23. Joad JP, Kott KS, and Bric JM. The local C-fiber contribution to ozone-induced effects on the isolated guinea pig lung. Toxicol Appl Pharmacol 141: 561-567, 1996.[ISI][Medline]
  24. Joad JP, McDonald RJ, Giri SN, and Bric JM. Ozone effects on mechanics and arachidonic acid metabolite concentrations in isolated rat lungs. Environ Res 66: 186-197, 1994.[Medline]
  25. Koto H, Aizawa H, Takata S, Inoue H, and Hara N. An important role of tachykinins in ozone-induced airway hyperresponsiveness. Am J Respir Crit Care Med 151: 1763-1769, 1995.[Abstract]
  26. Lee LY, Bleecker ER, and Nadel JA. Effects of ozone on the bronchomotor response to inhaled histamine aerosol in dogs. J Appl Physiol 43: 626-631, 1977.[Abstract/Free Full Text]
  27. Lundberg JM, Hokfelt T, Martling CR, Saria A, and Cuello C. Substance P-immunoreactive sensory nerves in the lower respiratory tract of various mammals including man. Cell Tissue Res 235: 251-261, 1984.[ISI][Medline]
  28. Lundberg JM and Saria A. Capsaicin-induced desensitization of airway mucosa to cigarette smoke, mechanical and chemical irritants. Nature 302: 251-253, 1983.[Medline]
  29. Lundberg JM, Saria A, Brodin E, Rosell S, and Folkers K. A substance P antagonist inhibits vagally induced increase in vascular permeability and bronchial smooth muscle contraction in the guinea pig. Proc Natl Acad Sci USA 80: 1120-1124, 1983.[Abstract/Free Full Text]
  30. Maggi CA, Santicioli P, Geppetti P, Giuliani S, Patacchini R, Frilli S, Grassi J, and Meli A. Involvement of a peripheral site of action in the early phase of neuropeptide depletion following capsaicin desensitization. Brain Res 436: 402-406, 1987.[ISI][Medline]
  31. Martins MA, Shore SA, and Drazen JM. Release of tachykinins by histamine, methacholine, PAF, LTD4, and substance P from guinea pig lungs. Am J Physiol Lung Cell Mol Physiol 261: L449-L455, 1991.[Abstract/Free Full Text]
  32. Mitchell RA, Herbert DA, Baker DG, and Basbaum CB. In vivo activity of tracheal parasympathetic ganglion cells innervating tracheal smooth muscle. Brain Res 437: 157-160, 1987.[ISI][Medline]
  33. Myers AC and Undem BJ. Analysis of preganglionic nerve evoked cholinergic contractions of the guinea pig bronchus. J Auton Nerv Syst 35: 175-184, 1991.[ISI][Medline]
  34. Myers AC and Undem BJ. Functional interactions between capsaicin-sensitive and cholinergic nerves in the guinea pig bronchus. J Pharmacol Exp Ther 259: 104-109, 1991.[Abstract/Free Full Text]
  35. Myers AC and Undem BJ. Electrophysiological effects of tachykinins and capsaicin on guinea-pig bronchial parasympathetic ganglion neurones. J Physiol 470: 665-679, 1993.[Abstract/Free Full Text]
  36. Myers AC, Undem BJ, and Weinreich D. Electrophysiological properties of neurons in guinea pig bronchial parasympathetic ganglia. Am J Physiol Lung Cell Mol Physiol 259: L403-L409, 1990.[Abstract/Free Full Text]
  37. Ollerenshaw S, Jarvis D, Woolcock A, Sullivan C, and Scheibner T. Absence of immunoreactive VIP in the tissue from the lungs of patients with asthma. N Engl J Med 320: 1244-1248, 1989.[Abstract]
  38. Pino MV, Levin JR, Stovall MY, and Hyde DM. Pulmonary inflammation and epithelial injury in response to acute ozone exposure in the rat. Toxicol Appl Pharmacol 112: 64-72, 1992.[ISI][Medline]
  39. Reynolds PN, Holmes MD, and Scicchitano R. Role of tachykinins in bronchial hyper-responsiveness. Clin Exp Pharmacol Physiol 24: 273-280, 1997.[ISI][Medline]
  40. Schelegle ES, Carl ML, Coleridge HM, Coleridge JCG, and Green JF. Contribution of vagal afferents to respiratory reflexes evoked by acute inhalation of ozone in dogs. J Appl Physiol 74: 2338-2344, 1993.[Abstract/Free Full Text]
  41. Sekizawa K, Tamaoki J, Nadel JA, and Borson DB. Enkephalinase inhibitor potentiates substance P- and electrically induced contraction in ferret trachea. J Appl Physiol 63: 1401-1405, 1987.[Abstract/Free Full Text]
  42. Shore SA, Schwartzman IN, Le Blanc B, Murthy GG, and Doerschuk CM. Tumor necrosis factor receptor 2 contributes to ozone-induced airway hyperresponsiveness in mice. Am J Respir Crit Care Med 164: 602-607, 2001.[Abstract/Free Full Text]
  43. Sterner-Kock A, Vesely KR, Stovall MY, Schelegle ES, Green JF, and Hyde DM. Neonatal capsaicin treatment increases the severity of ozone-induced lung injury. Am J Respir Crit Care Med 153: 436-443, 1996.[Abstract]
  44. Watson N, Maclagan J, and Barnes PJ. Endogenous tachykinins facilitate transmission through parasympathetic ganglia in guinea-pig trachea. Br J Pharmacol 109: 751-759, 1993.[ISI]
  45. Wood JN, Winter J, James IF, Rang HP, Yeats J, and Bevan S. Capsaicin-induced ion fluxes in dorsal root ganglion cells in culture. J Neurosci 8: 3208-3220, 1988.[Abstract]
  46. Wu ZX and Lee LY. Airway hyperresponsiveness induced by chronic exposure to cigarette smoke in guinea pigs: role of tachykinins. J Appl Physiol 87: 1621-1628, 1999.[Abstract/Free Full Text]
  47. Wu ZX, Maize DF Jr, Satterfield BE, Frazer DG, Fedan JS and Dey RD. Role of intrinsic airway neurons in ozone-induced airway hyperresponsiveness in ferret trachea. J Appl Physiol 91: 371-378, 2001.[Abstract/Free Full Text]
  48. Wu ZX, Morton RF, and Lee LY. Role of tachykinins in ozone-induced airway hyperresponsiveness to cigarette smoke in guinea pigs. J Appl Physiol 83: 958-965, 1997.[Abstract/Free Full Text]
  49. Wu ZX, Satterfield BE, Fedan JS, and Dey RD. Interleukin-1{beta}-induced airway hyperresponsiveness enhances substance P in intrinsic neurons of ferret airway. Am J Physiol Lung Cell Mol Physiol 283: L909-L917, 2002.[Abstract/Free Full Text]
  50. Zhu W and Dey RD. Projections and pathways of VIP- and nNOS-containing airway neurons in ferret trachea. Am J Respir Cell Mol Biol 24: 38-43, 2001.[Abstract/Free Full Text]



This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. S. Hazari, J. H. Pan, and A. C. Myers
Nerve growth factor acutely potentiates synaptic transmission in vitro and induces dendritic growth in vivo on adult neurons in airway parasympathetic ganglia
Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L992 - L1001.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
95/2/742    most recent
00109.2003v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wu, Z.-X.
Right arrow Articles by Dey, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, Z.-X.
Right arrow Articles by Dey, R. D.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.