Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 91: 100-108, 2001;
8750-7587/01 $5.00
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fulco, C. S.
Right arrow Articles by Lewis, S. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fulco, C. S.
Right arrow Articles by Lewis, S. F.
Vol. 91, Issue 1, 100-108, July 2001

Gender alters impact of hypobaric hypoxia on adductor pollicis muscle performance

Charles S. Fulco1, Paul B. Rock1, Stephen R. Muza1, Eric Lammi1, Barry Braun2, Allen Cymerman1, Lorna G. Moore3, and Steven F. Lewis4

1 Thermal and Mountain Medicine Division, United States Army Research Institute of Environmental Medicine, Natick, Massachusetts 01760; 2 Veterans Affairs Medical Center and Stanford University, Palo Alto, California 94304; 3 University of Colorado Health Sciences Center, Denver, Colorado 80262; and 4 Sargent College of Health and Rehabilitation Sciences, Boston University, Boston, Massachusetts 02215


    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES

Recently, we reported that, at similar voluntary force development during static submaximal intermittent contractions of the adductor pollicis muscle, fatigue developed more slowly in women than in men under conditions of normobaric normoxia (NN) (Acta Physiol Scand 167: 233-239, 1999). We postulated that the slower fatigue of women was due, in part, to a greater capacity for muscle oxidative phosphorylation. The present study examined whether a gender difference in adductor pollicis muscle performance also exists during acute exposure to hypobaric hypoxia (HH; 4,300-m altitude). Healthy young men (n = 12) and women (n = 21) performed repeated static contractions at 50% of maximal voluntary contraction (MVC) force of rested muscle for 5 s followed by 5 s of rest until exhaustion. MVC force was measured before and at the end of each minute of exercise and at exhaustion. Exhaustion was defined as an MVC force decline to 50% of that of rested muscle. For each gender, MVC force of rested muscle in HH was not significantly different from that in NN. MVC force tended to decline at a faster rate in HH than in NN for men but not for women. In both environments, MVC force declined faster (P < 0.01) for men than for women. For men, endurance time to exhaustion was shorter (P < 0.01) in HH than in NN [6.08 ± 0.7 vs. 8.00 ± 0.7 (SE) min]. However, for women, endurance time to exhaustion was similar (not significant) in HH (12.86 ± 1.2 min) and NN (13.95 ± 1.0 min). In both environments, endurance time to exhaustion was longer for women than for men (P < 0.01). Gender differences in the impact of HH on adductor pollicis muscle endurance persisted in a smaller number of men and women matched (n = 4 pairs) for MVC force of rested muscle and thus on submaximal absolute force and, by inference, ATP demand in both environments. In contrast to gender differences in the impact of HH on small-muscle (adductor pollicis) exercise performance, peak O2 uptake during large-muscle exercise was lower in HH than in NN by a similar (P > 0.05) percentage for men and women (-27.6 ± 2 and -25.1 ± 2%, respectively). Our findings are consistent with the postulate of a higher adductor pollicis muscle oxidative capacity in women than in men and imply that isolated performance of muscle with a higher oxidative capacity may be less impaired when the muscle is exposed to HH.

strength; endurance; muscle fatigue; exhaustion; oxidative capacity; altitude; small-muscle exercise; peak oxygen uptake


    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES

IN MEN AND WOMEN, ENDURANCE performance during large-muscle exercise (e.g., bicycle ergometer or treadmill) is markedly impaired by acute exposure to hypobaric and normobaric hypoxia (13). This impairment has been closely linked to a similar magnitude of reduction in maximal aerobic power for each gender (e.g., -25 to -30% at 4,300-m altitude) because of arterial hypoxemia (13). In men, under comparable hypoxic conditions, exercise utilizing smaller muscles [e.g., knee extensors (12) and adductor pollicis (11)] tends to be associated with prominent, although smaller degrees of, impairment of arterial oxygen saturation, peak oxygen uptake (VO2 peak), and endurance performance. In contrast, less is known regarding the effects of hypoxia on small-muscle exercise performance in women.

We recently observed a slower development of fatigue, a longer endurance time to exhaustion, and a faster early recovery of an isolated small-muscle, the adductor pollicis, in women than in men associated with high-intensity, intermittent static contractions performed under normoxic conditions (14). Because the adductor pollicis muscle of men and women contains a uniformly high percentage of slow-twitch high-oxidative-capacity fibers (21, 33), our observations led us to postulate (14) that the superior muscle performance of women under exercise conditions involving complete motor unit recruitment (24, 29) was linked partly to a greater capacity for oxidative phosphorylation in the fast-twitch fibers of women's adductor pollicis muscle. For a given ATP requirement during exercise, an isolated muscle that has a greater potential for oxidative phosphorylation, as reported for the vastus lateralis muscle of women compared with men (30, 37), is likely to be less reliant on anaerobic pathways and reactions that cause local metabolic conditions associated with impaired muscle contractile performance.

In the present study, we tested the hypothesis that voluntary performance during high-intensity, intermittent static contractions of the adductor pollicis muscle is less impaired during acute exposure to hypobaric hypoxia in women than in men. Our hypothesis was tested by comparing adductor pollicis muscle performance in men and women under conditions of normobaric normoxia and hypobaric hypoxia. The rationale for this hypothesis was based on findings that, taken together, imply that muscle with a higher oxidative capacity may more effectively regulate muscle oxidative phosphorylation under hypoxic conditions (2, 5, 7, 19).

In addition to the adductor pollicis muscle experiments, the present subjects also performed conventional large-muscle dynamic exercise to VO2 peak under the same environmental conditions. Comparison of small-muscle intermittent static exercise and large-muscle dynamic exercise under normobaric and hypobaric conditions allowed us to more closely link the adductor pollicis muscle performance findings with physiological conditions specific to this muscle group and its mode of exercise.


    METHODS
TOP
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES

Subjects

Twenty-one healthy women with normal menstrual cycles and no history of oral contraceptive use or pregnancy in the preceding year and 12 healthy men gave written informed consent before participating in the study. For the women, no statistically significant differences in adductor pollicis muscle performance were observed between their follicular and luteal menstrual cycle phases in normoxia or in hypobaric hypoxia (unpublished observations). Age, height, and weight (mean ± SE) were 22 ± 1 yr, 166 ± 1 cm, and 65 ± 2 kg for the women and 29 ± 1 yr, 180 ± 2 cm, and 80 ± 3 kg for the men. All male and female subjects were right-hand dominant.

In response to a questionnaire regarding participation in physical activity and sport, the 12 men reported exercising 23 ± 1 days/mo (range 16-28 days, median 24 days) in activities such as running, bicycling, football, soccer, kayaking, and volleyball. Similarly, the 21 women reported participating 19 ± 2 days/mo (range 4-28 days, median 24 days) in activities such as running, bicycling, walking, swimming, and badminton.

Study Locations

Women performed normobaric normoxia muscle testing at the Geriatric Research Education and Clinical Center of the Palo Alto Veterans Affairs Medical Center (Palo Alto, CA; altitude 30 m). Men performed normobaric normoxia testing at the US Army Research Institute of Environmental Medicine (Natick, MA; altitude 50 m). Women and men were tested under conditions of hypobaric hypoxia in a laboratory on the summit of Pikes Peak, CO (4,300-m altitude, 464 Torr). For both genders, testing in normoxia always preceded testing in hypoxia, with >= 2 wk between testing at each location. Subjects were transported via airplane and automobile to Pikes Peak from California or Massachusetts in <6 h. For all subjects, adductor pollicis muscle experiments were conducted within the first 24 h and VO2 peak tests within the 1st wk of altitude exposure. At both locations, for the adductor pollicis muscle experiments, the identical muscle apparatus, testing and calibration procedures, force transducer, and force feedback display were used by the same investigators. Ambient temperature was maintained in a comfortable range (20-23°C) at each location.

General Experimental Design and Procedures

Adductor pollicis muscle testing. To ensure familiarization with all equipment, personnel, and procedures and to learn to execute intermittent static contractions exclusively with the adductor pollicis muscle, subjects underwent at least one adductor pollicis muscle familiarization session before the definitive experiments. Familiarization sessions consisted of practicing repeated submaximal static contractions interspersed with periodic maximal contractions.

Definitive muscle fatigue experiments were performed using a device that permitted static contractions isolated to the adductor pollicis muscle (11, 27). The right hand and arm of the subject were secured in supination with the fingers flexed and thumb abducted. A force transducer (model SSM-250, Interface, Scottsdale, AZ; sensitivity 1.5 mV/kg) was attached by an inextensible link to a strap looped around the interphalangeal joint of the right thumb. The force transducer was interfaced with an amplifier (model 13-421202, Gould, Cleveland, OH; 90% response time in 2 ms), chart recorder (model 2200, Gould), and oscilloscope. Subjects had visual contact with the oscilloscope tracings at all times to provide them with feedback for maintaining the correct force during submaximal contractions.

After the subject was seated and the hand and thumb were properly oriented and secured, three 5-s baseline maximal voluntary contractions (MVCs) were performed. There was a 1-min rest between each MVC. For each subject, the highest MVC force attained (MVC force of rested muscle, i.e., strength) was used to set the target force of submaximal contractions.

After an additional 1-min rest, exercise was initiated with an MVC, the subjects rested for 5 s, and then the first submaximal contraction was performed. Submaximal exercise consisted of intermittent, 5-s static muscle contractions at a target force of 50% of rested MVC force followed by 5 s of rest (i.e., duty cycle 0.5). At the end of every minute (i.e., every 6th contraction), an MVC was performed for the full 5 s instead of the 50% MVC force contraction. An investigator timing the events verbally instructed the subjects to start and stop each submaximal and maximal contraction. During each maximal or submaximal contraction, subjects were required to increase muscle force (in N) as rapidly as possible to the maximal or target level, respectively. When MVC force fell to or below target or the target force could not be maintained for 5 s, the subjects were considered exhausted and were instructed to stop the submaximal contractions. An MVC was performed immediately on reaching exhaustion. Endurance time to exhaustion was calculated as the time from the start of the MVC of rested muscle immediately preceding the first submaximal contraction to the end of the MVC performed at the point of exhaustion. Figure 1 illustrates the study protocol and specific measurements obtained. For the women, arterial oxygen saturation was continuously determined by pulse oximetry of a finger on the left hand in normoxia and hypoxia (model N-200 pulse oximeter, Nellcor, Pleasanton, CA).


View larger version (41K):
[in this window]
[in a new window]
  		Fig. 1.   Schematic of study protocol and measurements. "Strength" is the highest force obtained in a maximal voluntary contraction (MVC) of rested muscle before starting repeated submaximal contractions. Submaximal "target force" is 50% of strength level. Each target force contraction lasts for 5 s and is followed by 5 s of rest. At the end of every minute (or for every 6th contraction), an MVC is performed for the full 5 s. "Rate of fatigue" is defined as the decline of MVC force relative to exercise duration resulting from the target force contractions. "Exhaustion" occurs when the 50% target force cannot be maintained for 5 s or MVC force falls to 50%. "Endurance time to exhaustion" is the time interval from the start of exercise to the exhaustion point. [Modified from Fulco et al. (14).]

VO2 peak. For the men, VO2 peak was determined in normoxia and hypoxia by a progressive-intensity, continuous treadmill running test to volitional exhaustion. The men warmed up by walking for 10 min (1.56 m/s) at a 10% treadmill grade. In normoxia, if heart rate was >140 beats/min during warm-up, the treadmill speed for the remainder of the test was set at a running speed of 2.68 m/s; if heart rate was <140 beats/min, the speed was set at 3.13 m/s. For running, the initial incline was 5% and was increased by 2.5% at the end of every 1.5 min. In hypoxia, the treadmill test was identical, except the lower running speed (2.68 m/s) was maintained constant for each man. For the women, VO2 peak was determined in normoxia and hypoxia by a progressive-intensity, continuous cycle ergometer test to volitional exhaustion. The women exercised on an electrically braked ergometer at a cadence of 70 rpm, beginning at 40 W and increasing by 25 W at the end of every 2 min. For men and women, oxygen uptake was measured continuously during exercise using calibrated metabolic carts (SensorMedics, Fullerton, CA).

Data analyses. For each subject, in each environment, overall muscle fatigue rate was calculated from the slope of individual linear regression analysis of the fall in MVC force (in N) relative to exercise time (in min). Mean slopes (fatigue rates) were calculated for each gender in each environment. For each gender, paired t-tests were used to identify normoxia vs. hypoxia differences in MVC force of rested muscle, absolute force developed during submaximal exercise, muscle fatigue rate (in N/min), endurance time to exhaustion, MVC force at exhaustion, and VO2 peak. For each of these dependent variables, differences between genders in each environment were determined using independent t-tests for unequal variances. For these within- and between-gender comparisons, the Bonferroni adjustment procedure was used to correct for potential type 1 errors due to multiple t-tests. P < 0.0125 was therefore accepted as statistically significant after these adjustments were made. Three-way ANOVAs (environment × exercise time × gender) with repeated measures on two factors (environment and exercise time) were used to identify gender differences in MVC force during exercise (i.e., percent decline in the first 3 min of exercise). If an ANOVA identified a significant F value, Tukey's multiple- comparison procedure was used to detect statistical significance of specific differences. For the analyses based on the ANOVAs, a difference of P < 0.05 was accepted as statistically significant. Values are means ± SE or individual values.


    RESULTS
TOP
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES

MVC Force of Rested Muscle

MVC force of rested adductor pollicis muscle was similar in normoxia and hypoxia for men [143 ± 5 and 152 ± 4 N, respectively, P = not significant (NS)] and women (111 ± 4 and 115 ± 5 N, respectively, P = NS). In normoxia and hypoxia, MVC force of rested muscle was greater for men than for women (P < 0.01).

Adductor Pollicis Muscle Force Exerted During Submaximal Exercise

The percentage of rested MVC force used during submaximal adductor pollicis muscle exercise was similar (both P = NS) in normoxia and hypoxia for men (51.9 ± 1 and 51.0 ± 1%, respectively) and women (52.3 ± 1 and 53.0 ± 1%, respectively). In each environment, the percentage of rested MVC force used during submaximal adductor pollicis muscle exercise was similar (P = NS) for men and women. Absolute force developed during submaximal adductor pollicis muscle exercise was similar (both P = NS) in normoxia and hypoxia for men (74 ± 2 and 77 ± 2 N, respectively) and women (58 ± 3 and 61 ± 3 N, respectively). In contrast, absolute force developed during submaximal exercise was greater for men than for women in normoxia and hypoxia (both P < 0.01).

Adductor Pollicis Muscle Fatigue Rate

Overall muscle fatigue rate tended to be faster in hypoxia than in normoxia for men (-13 ± 2 vs. -8 ± 2 N/min, respectively, P < 0.05) but not for women (-5 ± 1 vs. -4 ± 1 N/min, respectively, P = NS). Overall fatigue rate for men was ~2-fold faster in normoxia (-8 ± 2 vs. -4 ± 1 N/min, respectively, P < 0.01) and ~2.5-fold faster in hypoxia (-13 ± 2 vs. -5 ± 1 N/min, respectively, P < 0.01) for men than for women.

First Three Minutes of Exercise

For each gender, the decline of MVC force from rest to the end of minute 3 of adductor pollicis muscle exercise was similar (P = NS) in normoxia and hypoxia (Fig. 2). By the end of minute 1 of exercise, MVC force had, from rest (i.e., 100% MVC), fallen nearly threefold more for men than for women in normoxia (-21 ± 2 vs. -6 ± 1%, P < 0.01) and hypoxia (-23 ± 3 vs. -8 ± 1%, P < 0.01). During the next 2 min of exercise in each environment, MVC force continued to decline for both genders. By the end of minute 3 of exercise in each environment, the difference between women and men in MVC force, expressed as a percentage of MVC force of rested muscle, remained similar to that at the end of minute 1.


View larger version (12K):
[in this window]
[in a new window]
  		Fig. 2.   Force of MVC expressed as a percentage of the MVC force of rested muscle for men (n = 12) and women (n = 21) during the first 3 min of intermittent static adductor pollicis muscle contraction under normoxic (A) and hypoxic (B) conditions. For each minute of exercise in each environment, the percentage of rested MVC force was markedly higher (P < 0.01) for women than for men. In addition, for each gender, the decline in percentage of rested MVC force from rest to the end of minute 3 of exercise was similar in normoxia and hypoxia [P = not significant (NS)]. *P < 0.01.

Progression Toward Exhaustion

Percentages of men and women remaining (i.e., those not reaching exhaustion) after each minute for the first 12 min of exercise in normoxia and hypoxia are shown in Fig. 3. In normoxia, all men were able to exercise for >= 4 min. However, the percentage of men remaining each minute declined steadily from minute 4 to minute 12. After minute 12 of normoxic exercise, 100% of the men had reached their point of exhaustion. In hypoxia, the percentage of men remaining at each minute between minute 4 and minute 10 was 8-50% (median 25%) lower than in normoxia.


View larger version (47K):
[in this window]
[in a new window]
  		Fig. 3.   Number of men (A) and women (B) remaining (i.e., had not yet reached exhaustion) after each minute of exercise under normoxic and hypoxic conditions.

In contrast to men in normoxia, all women in normoxia were able to exercise for >= 8 min, and 57% of women remained by minute 12. The percentage of women remaining each minute declined progressively, but sharply higher percentages of women than men remained exercising beyond minute 4. Moreover, in direct contrast to the much faster rate of dropout during hypoxic than normoxic exercise in men, the percentage of women remaining after each minute of exercise tended to be similar in hypoxia and normoxia. When the progression toward exhaustion in hypoxia was compared with that in normoxia, the percentage of women remaining at each minute between minute 4 and minute 10 was only 0-23% (median 9%) lower in hypoxia.

Adductor Pollicis Muscle Endurance Time to Exhaustion

For men, endurance time to exhaustion was shorter (P < 0.01) in hypoxia (6.08 ± 0.7 min) than in normoxia (8.00 ± 0.7 min); for women, endurance time was similar (P = NS) in both environments (12.86 ± 1.2 min in hypoxia vs. 13.95 ± 1.0 min in normoxia). Endurance time to exhaustion was longer (P < 0.01) for women than for men in normoxia and hypoxia (Fig. 4).


View larger version (19K):
[in this window]
[in a new window]
  		Fig. 4.   Adductor pollicis muscle endurance time to exhaustion for men and women in normoxia and hypoxia. For each environment, women had a significantly longer endurance time than men (P < 0.01). Endurance time was reduced for men (P < 0.01) but not for women (P = NS) in hypoxia compared with normoxia.

MVC Force at Exhaustion

At exhaustion, MVC force had fallen to a similar (P = NS) percentage of MVC force of rested muscle in normoxia and hypoxia for men (55 ± 1 and 53 ± 2%, respectively) and women (53 ± 1 and 55 ± 1%, respectively). In addition, MVC force fell to a similar (P = NS) percentage of MVC force of rested muscle at the point of exhaustion for men and women in normoxia and hypoxia.

Arterial Oxygen Saturation During Adductor Pollicis Muscle Exercise

In normoxia, women's arterial oxygen saturation was 97 ± 1% at rest and remained about the same (i.e., ±1%) over the duration of adductor pollicis muscle exercise and recovery. In hypobaric hypoxia, women's arterial oxygen saturation was 85 ± 1% during seated rest and rose to 88 ± 1% at exhaustion from adductor pollicis muscle exercise (P < 0.05). During rest and exercise, women's arterial oxygen saturation was lower in hypobaric hypoxia than in normoxia (P < 0.01).

VO2 peak

For each gender, VO2 peak was lower (P < 0.01) in hypobaric hypoxia than in normoxia. VO2 peak declined by a similar percentage (P = NS) from normoxic to hypobaric hypoxic conditions for men (from 56.5 ± 1 to 40.7 ± 1 ml · kg-1 · min-1, i.e., -27.6 ± 2%) and women (from 40.4 ± 1 to 30.1 ml · kg-1 · min-1, i.e., -25.1 ± 2%).

Gender Pairs Matched for MVC Force

To examine whether the gender difference in the impact of hypobaria on adductor pollicis muscle performance was related to a lower muscle energy demand for women, we matched, after completion of muscle testing, an equal number (n = 4) of women and men as closely as possible for MVC force of rested muscle in normoxia and hypoxia. Matching was accomplished by ranking and comparing the results for each gender in normoxia and hypoxia and then progressively discarding data for some women and men until mean MVC force did not differ (P = NS) within and between genders for both environments. As a result of matching on MVC force of rested muscle, the submaximal contraction forces produced in normoxic and hypoxic conditions by the men were 74 ± 3 and 76 ± 1 N, respectively, and those produced by the women were 73 ± 4 and 76 ± 8 N, respectively. Our matching procedure therefore enabled men and women to be compared at similar (P = NS) absolute submaximal contraction forces and, by inference, similar muscle ATP demand, in normoxia and hypoxia.

At a similar absolute submaximal contraction force, these men were unable to maintain MVC force as well as women (P < 0.01) at the end of minute 1 of exercise in normoxia (84 ± 3 vs. 95 ± 2% MVC force of rested muscle, respectively) and hypoxia (77 ± 4 vs. 92 ± 4% MVC force of rested muscle, respectively; Fig. 5A). During the next 2 min of exercise in each environment, MVC force continued to decline for both genders. By the end of minute 3 of exercise, MVC force relative to that of rested muscle fell in normoxia to 74 ± 1% for men and 82 ± 3% for women and in hypoxia to 64 ± 8% for men and 82 ± 3% for women [genders tended to differ (P < 0.05) in both environments]. The women also tended to have longer muscle endurance times than men (P < 0.05) in normoxia (11.00 ± 2 vs. 8.00 ± 1 min) and hypoxia (12.50 ± 3 vs. 5.50 ± 1 min; Fig. 5B). In addition, for men, endurance times to exhaustion tended to be shorter (P < 0.05) in hypoxia (5.50 ± 1 min) than in normoxia (8.00 ± 1 min), but for women endurance times were similar (P = NS) in both environments (12.50 ± 3 and 11.00 ± 2 min in hypoxia and normoxia, respectively).


View larger version (13K):
[in this window]
[in a new window]
  		Fig. 5.   A: adductor pollicis muscle MVC force, expressed as percent MVC force of rested muscle, after minute 1 of submaximal adductor pollicis muscle contractions in each of the 4 pairs of men and women matched for MVC force of rested muscle in normoxia and hypoxia. Each woman maintained a higher percentage of MVC force than each man under normoxic and hypoxic conditions. B: adductor pollicis muscle endurance time to exhaustion in each of the 4 pairs of men and women matched for MVC force of rested muscle in normoxia and hypoxia. For 3 of 4 matched pairs in normoxia and for all 4 pairs in hypoxia, each woman had a longer endurance time than each man. Alphanumeric characters represent the 4 male-female pairs matched on MVC force of rested adductor pollicis muscle: N, normoxia; H, hypoxia; 1-4, numbers of the matched pairs. Male-female pairs are compared with a line of identity (diagonal line).


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES

Our major finding confirmed the hypothesis that voluntary performance during intermittent static contractions of the adductor pollicis muscle is less impaired during acute exposure to hypobaric hypoxia in women than in men. Moreover, under hypoxic conditions, women did not display the increase in overall fatigue rate and decrease in endurance time to exhaustion found in men. To our knowledge, this finding represents the first direct demonstration of a gender difference in the impact of hypoxia on human muscle performance. In contrast to our adductor pollicis muscle observations, for large-muscle exercise in hypoxia, each gender demonstrated a similar degree of decline of VO2 peak from normoxic values. In this discussion, we identify the most likely reasons for the distinct gender difference in the effect of hypoxia on performance during adductor pollicis muscle exercise, explain the physiological significance of this observation, and distinguish our adductor pollicis muscle findings from those of more conventional large-muscle effort.

Our specific exercise protocol and the unique characteristics of the adductor pollicis muscle likely contributed to the present findings. The protocol involved static contractions performed at 50% of MVC force in a 5-s activity-5-s recovery duty cycle. This type of exercise is fueled largely by muscle high-energy phosphate breakdown during contractions and resynthesis of high-energy phosphates by oxidative phosphorylation during recovery (6, 41). In men and women, the adductor pollicis muscle contains a uniformly high percentage (~80%) of slow-twitch, high-oxidative-capacity fibers (21, 33). In addition, during adductor pollicis muscle contractions at 50% of MVC force, virtually all motor units are recruited (24, 29), and the rapid and repeated generation of this force in the present protocol likely augmented the need for fast-twitch fiber recruitment (10, 18). Our finding of a markedly slower development of adductor pollicis muscle fatigue for women than for men after only 1 min of exercise under normoxic conditions is supported by earlier observations (14) and is postulated to be linked with a greater capacity for oxidative phosphorylation in the fast-twitch fibers of the adductor pollicis muscle of women. A greater fast-twitch fiber oxidative capacity would promote more rapid resynthesis of muscle high-energy phosphate between contractions and result in more complete removal of products of high-energy phosphate breakdown such as Pi and ADP, accumulations of which have been associated with impaired muscle performance (3, 35).

Our finding of a similar adductor pollicis muscle performance for women in hypoxia and normoxia is consistent with the hypothesis of a relatively high oxidative capacity in the fast-twitch fibers of the adductor pollicis muscle of women. When compared in hypoxia vs. normoxia, each gender performed adductor pollicis muscle contractions at a similar absolute force and thus a similar absolute muscle ATP requirement. Muscle with increased mitochondrial content can produce a given amount of ATP via oxidative phosphorylation with reduced concentrations of key metabolic regulators, e.g., ADP (5) or substrate (19). A prominent theory for control of oxidative phosphorylation in active muscle holds that regulation may be exerted via interaction between the muscle phosphorylation potential ([ATP]/[ADP][Pi], where [ATP], [ADP], and [Pi] are concentrations of ATP, ADP, and Pi, respectively), the mitochondrial redox state ([NADH]/[NAD+], where [NADH] and [NAD+] are concentrations of NADH and NAD+, respectively), and effectors of cytochrome oxidase such as oxygen (7). In this control scheme, the relative impact of [ATP]/[ADP][Pi], [NADH]/[NAD+], and oxygen depends on specific metabolic conditions prevailing in active muscle. Under hypoxic conditions, changes in [ATP]/[ADP][Pi] and [NADH]/[NAD+] can, within limits, compensate for decreased PO2, leaving ATP flux constant (2). The present finding of a lack of effect of hypoxia on women's adductor pollicis muscle performance supports the hypothesis that muscle with a greater intrinsic oxidative capacity has a greater ability to sustain a given rate of ATP production via oxidative phosphorylation with smaller compensatory changes in [ATP]/[ADP][Pi] and [NADH]/[NAD+]. This property would reduce activation of anaerobic pathways and reactions that lead to metabolic conditions associated with accelerated muscle fatigue under hypoxic conditions.

In normoxia and hypoxia, mean MVC force of rested adductor pollicis muscle for the group of 12 men was ~30% higher than that for the group of 21 women. Correspondingly, during submaximal contractions performed at 50% of individual MVC force, absolute force developed, and, by inference, muscle ATP demand averaged ~26-28% higher for men than for women in normoxia and hypoxia. To examine whether the gender difference in the impact of hypoxia on adductor pollicis muscle performance was related to a lower muscle energy demand for women, we matched as many men and women as possible (n = 4 pairs) with respect to MVC force of rested muscle and, therefore, on absolute force development during each submaximal contraction in normoxia and hypoxia. Our observations of muscle performance for women that was superior to that for men in normoxia and hypoxia under conditions of matched force and, likely, similar muscle ATP demand support the hypothesis that, compared with men, the adductor pollicis muscle of women can, during exposure to hypoxia, more effectively regulate oxidative phosphorylation and, thereby, lessen muscle metabolic conditions associated with fatigue.

In addition to local factors in active muscle, neural processes that may contribute to fatigue development (15) could differ between women and men (36). Neural factors that could affect the present findings of slower fatigue development in women than in men in normoxia and hypoxia may relate to a potential female-male difference in central motor drive, neuromuscular propagation, and/or a fall in neural excitation of muscle of reflex origin. There is, however, little or no published evidence supporting a major role for the first two factors under the present experimental conditions. Almost 50 years ago, Merton (27) reported that, for adductor pollicis muscle, voluntary activation could account for virtually all force production in fresh and fatigued muscle. Using more sensitive techniques, Herbert and Gandevia (20) recently observed that voluntary activation could explain ~90% of voluntary force production in adductor pollicis muscle with no difference between genders. For adductor pollicis (11) and other muscle groups (1, 12, 17, 42), there is little or no evidence for impairment of central motor drive due to hypoxic conditions similar to those of the present study. In addition, virtually no published data link moderate or severe hypoxia (17) or gender with impaired neuromuscular propagation.

Activation of metabolically sensitive group III and IV afferent nerve endings in fatigued muscles has been implicated in a reflex decline in motoneuron discharge to active muscle (16) via reduction of motor drive in the central nervous system (15) or spinal cord (31). A reflex increase in sympathetic nerve activity to inactive muscle is a prominent manifestation of group III and IV metaboreflex activity (40). Accumulation of metabolites such as H+, Pi, and H2PO<UP><SUB>4</SUB><SUP>−</SUP></UP> in active muscle has been linked with impaired force generation (3, 28, 35), excitation of group III and IV muscle afferents (16), and reflex increases in sympathetic nerve activity (26, 39, 40). Moreover, there is published evidence that 1) accumulation of these metabolites and reflex increases in sympathetic nerve activity are reduced by interventions to increase muscle oxidative capacity (5, 22, 38) and 2) during adductor pollicis muscle exercise, reflex increases in muscle sympathetic nerve activity are smaller in women than in men (8). The above findings therefore support the hypothesis that a better voluntary performance of the adductor pollicis muscle for women than for men under normoxic and hypobaric conditions is linked with a greater oxidative capacity of this muscle, which results in more slowly developing direct, intramuscular fatigue and a related decline in reflex impairment of muscle excitation.

Earlier findings (13) support our observation of similar percent declines from normoxic values in peak cycle ergometer or treadmill oxygen uptake in women and men. Thus the present finding of a gender difference in the effect of hypobaria on adductor pollicis muscle but not larger muscle performance in the same subjects would appear inconsistent. This potential discrepancy can be resolved by considering salient features of 1) systemic and local muscle oxygen transport in hypobaria and 2) the comparative energetics of large-muscle dynamic exercise vs. intermittent static contractions of the adductor pollicis muscle. These factors likely combine to create distinctly different local muscle metabolic states for the two exercise modes in similar hypobaric environments. In peak large-muscle dynamic exercise at 464 Torr, a high cardiac output and commensurately short pulmonary capillary red cell transit time (4) typically result in a 4-8% drop from resting values in arterial oxygen saturation (23) that aggravates the existing hypoxemia. In contrast, the small increase over resting values in arterial oxygen saturation we observed for hypobaric adductor pollicis muscle exercise in women is similar to previous small-muscle exercise findings under comparable hypobaric hypoxia conditions in men (12) and appears linked, in part, with only a minor increase in cardiac output typical for exercise engaging a small-muscle mass (25). For peak cycle or treadmill exercise, the local effect of the greater systemic hypoxemia (i.e., smaller muscle capillary-mitochondrial PO2 gradient) would be exacerbated by high muscle blood flow (34) and relatively brief muscle capillary red cell transit time associated with a combination of very high energy demand (9) and short recovery periods for oxidative resynthesis of ATP (9, 32) during rapidly repeated high-intensity dynamic contractions. By comparison, the lower ATP demand (9) and the duty cycle (i.e., 5 s of work-5 s of rest) of the static contractions performed in the adductor pollicis muscle exercise protocol may have provided optimal conditions for expression of an intrinsic gender difference in the effect of hypoxia on muscle fatigability.

In summary, adductor pollicis muscle performance during high-intensity, intermittent static contractions is less impaired in women than in men during acute exposure to hypobaric hypoxia. This finding contrasts with that of similar declines in VO2 peak during large-muscle dynamic exercise for both genders under hypoxic conditions. Unique properties of the adductor pollicis muscle that include, for both genders, a high percentage of slow-twitch high oxidative fibers and complete motor unit recruitment at the target force used and findings of a much smaller force loss for women than men after only 1 min of exercise in normoxia and hypoxia suggest that the superior muscle performance of women relates to a greater capacity for oxidative phosphorylation in their fast-twitch fibers. A greater fast-twitch fiber oxidative capacity would promote more rapid resynthesis of muscle high-energy phosphates between intense muscle contractions and faster removal of products of high-energy phosphate breakdown that have been linked with impaired muscle performance.


    ACKNOWLEDGEMENTS

We are extremely grateful to Ken W. Kambis, John T. Reeves, Beth Beidleman, Stacy Zamudio, and George A. Brooks, whose hard work and dedication allowed this project to proceed smoothly, and to all of our outstanding volunteers. Most of all, we are grateful to the late Gail Butterfield, who will always be remembered for her limitless dedication, enthusiasm, and good nature.


    FOOTNOTES

The work presented here was partly supported by Defense Women's Health Research Program Grant DI950050, awarded to the US Army Research Institute of Environmental Medicine, titled "Effect of Menstrual Cycle Phase on Muscle Fatigue and Physical Performance During Altitude Acclimatization," and Defense Women's Health Research Program Grant DAMD 17-94-BAA, awarded to the University of Colorado Health Sciences Center, titled "Women at Altitude: Effects of Menstrual Cycle Phase on High-Altitude Acclimatization."

The views, opinions, and/or findings contained in this report are those of the authors and should not be construed as an official Department of the Army position, policy, or decision unless so designated by other documentation.

Human subjects participated in these studies after giving their free and informed voluntary consent. Investigators adhered to US Army Regulation 70-25 and US Army Medical Research Materials Command Regulation 70-25 on the use of volunteers in research.

Citations of commercial organizations and trade names in this report do not constitute an official Department of the Army endorsement or approval of the products or services of these organizations.

Address for reprint requests and other correspondence: C. S. Fulco, Thermal and Mountain Medicine Div., USARIEM, Kansas St., Natick, MA 01760 (E-mail: charles.fulco{at}na.amedd.army.mil).

The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Received 28 November 2000; accepted in final form 12 February 2001.


    REFERENCES
TOP
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES

1.   Burse, RL, Cymerman A, and Young AJ. Respiratory response and muscle function during isometric handgrip exercise at high altitude. Aviat Space Environ Med 58: 39-46, 1987[Medline].

2.   Connett, RJ, Honig CR, Gayeski TEJ, and Brooks GA. Defining hypoxia: a systems view of VO2, glycolysis, energetics, and intracellular PO2. J Appl Physiol 68: 833-842, 1990[Abstract/Free Full Text].

3.   Cooke, R, and Pate E. The effects of ADP and phosphate on the contraction of muscle fibers. Biophys J 48: 789-798, 1985[Abstract/Free Full Text].

4.   Dempsey, JA, and Wagner PD. Exercise-induced arterial hypoxemia. J Appl Physiol 87: 1997-2006, 1999[Abstract/Free Full Text].

5.   Dudley, GA, Tullson PC, and Terjung RL. Influence of mitochondrial content on the sensitivity of respiratory control. J Biol Chem 262: 9109-9114, 1987[Abstract/Free Full Text].

6.   Edwards, RHT, Ekelund L-G, Harris RC, Hesser CM, Hultman E, Melcher A, and Wigertz O. Cardiorespiratory and metabolic costs of continuous and intermittent exercise in man. J Physiol (Lond) 234: 481-497, 1973[Abstract/Free Full Text].

7.   Erecinska, M, and Wilson DF. Regulation of cellular energy metabolism. J Membr Biol 70: 1-14, 1982[ISI][Medline].

8.   Ettinger, SM, Silber DH, Collins BG, Gray KS, Sutliff G, Whisler SK, McClain JM, Smith MB, Yang QX, and Sinoway LI. Influences of gender on sympathetic nerve responses to static exercise. J Appl Physiol 80: 245-251, 1996[Abstract/Free Full Text].

9.   Fenn, WO. A quantitative comparison between the energy liberated and the work performed by isolated sartorius muscle of the frog. J Physiol (Lond) 58: 175-203, 1923.

10.   Freund, HJ. Motor unit and muscle activity in voluntary motor control. Physiol Rev 63: 387-436, 1983[Free Full Text].

11.   Fulco, CS, Cymerman A, Muza SR, Rock PB, Pandolf KB, and Lewis SF. Adductor pollicis muscle fatigue during acute and chronic altitude exposure and return to sea level. J Appl Physiol 77: 179-183, 1994[Abstract/Free Full Text].

12.   Fulco, CS, Lewis SF, Frykman P, Boushel R, Smith S, Harman EA, Cymerman A, and Pandolf KB. Muscle fatigue and exhaustion during dynamic leg exercise in normoxia and hypobaric hypoxia. J Appl Physiol 81: 1891-1900, 1996[Abstract/Free Full Text].

13.   Fulco, CS, Rock PB, and Cymerman A. Maximal and submaximal exercise performance at altitude. Aviat Space Environ Med 69: 793-801, 1998[Medline].

14.   Fulco, CS, Rock PB, Muza SR, Lammi E, Cymerman A, Butterfield GE, Moore LG, Braun B, and Lewis SF. Slower fatigue and faster recovery of the adductor pollicis muscle in women matched for strength with men. Acta Physiol Scand 167: 233-239, 1999[ISI][Medline].

15.   Gandevia, SC. Neural control in human muscle fatigue: changes in muscle afferents, motoneurons, and motor cortical drive. Acta Physiol Scand 162: 275-283, 1998[ISI][Medline].

16.   Garland, SJ, and Kaufman MP. Role of muscle afferents in the inhibition of motoneurons during fatigue. In: Fatigue: Neural and Muscular Mechanisms, edited by Gandevia SC, Enoka RM, McComas AJ, Stuart DG, and Thomas CK.. New York: Plenum, 1995, p. 271-278.

17.   Garner, SH, Sutton JR, Burse RL, McComas AJ, Cymerman A, and Houston CS. Operation Everest II: neuromuscular performance under conditions of extreme simulated altitude. J Appl Physiol 68: 1167-1172, 1990[Abstract/Free Full Text].

18.   Gollnick, PD, Karlsson J, Piehl K, and Saltin B. Selective glycogen depletion in skeletal muscle fibres of man following sustained contractions. J Physiol (Lond) 241: 59-67, 1974[Abstract/Free Full Text].

19.   Gollnick, PD, and Saltin B. Significance of skeletal muscle oxidative enzyme enhancement with endurance training. Clin Physiol 2: 1-12, 1982[ISI][Medline].

20.   Herbert, RD, and Gandevia SC. Muscle activation in unilateral and bilateral efforts assessed by motor nerve and cortical stimulation. J Appl Physiol 80: 1351-1356, 1996[Abstract/Free Full Text].

21.   Johnson, MA, Polgar J, Weightman D, and Appleton D. Data on the distribution of fibre types in 36 human muscles. An autopsy study. J Neurol Sci 18: 111-129, 1973[ISI][Medline].

22.   Kent-Braun, JA, McCully KK, and Chance B. Metabolic effects of training in humans: a 31P-MRS study. J Appl Physiol 69: 1165-1170, 1990[Abstract/Free Full Text].

23.   Keul, J, Doll E, and Keppler D. Hypoxia and energy supply. In: Energy Metabolism of Human Muscle. Medicine and Sport, edited by Keul J, Doll E, and Keppler D.. Baltimore, MD: University Park, 1972, p. 203-243.

24.   Kukulka, CG, and Clamann HP. Comparison of the recruitment and discharge properties of motor units in human brachial biceps and adductor pollicis during isometric contractions. Brain Res 219: 45-55, 1981[ISI][Medline].

25.   Lewis, SF, Snell PG, Taylor WF, Hamra M, Graham RM, Pettinger WA, and Blomqvist CG. Role of muscle mass and mode of contraction in circulatory responses to exercise. J Appl Physiol 58: 146-151, 1985[Abstract/Free Full Text].

26.   Maclean, DA, Imadojenu VA, and Sinoway LI. Interstitial pH, K+, lactate, and phosphate determined with MSNA during exercise in humans. Am J Physiol Regulatory Integrative Comp Physiol 278: R563-R571, 2000[Abstract/Free Full Text].

27.   Merton, PA. Voluntary strength and fatigue. J Physiol (Lond) 123: 553-564, 1954.

28.   Miller, RG, Kent-Braun JA, Sharma KR, and Weiner MW. Mechanisms of human muscle fatigue: quantitating the contribution of metabolic factors and activation impairment. In: Fatigue: Neural and Muscular Mechanisms, edited by Gandevia SC, Enoka RM, McComas AJ, Stuart DG, and Thomas CK.. New York: Plenum, 1995, p. 195-210.

29.   Moritani, T, Muro M, Kijima A, and Berry MJ. Intramuscular spike analysis during ramp force and muscle fatigue. Electromyogr Clin Neurophysiol 26: 147-160, 1986[Medline].

30.   Nygaard, E. Skeletal muscle fibre characteristics in young women. Acta Physiol Scand 112: 299-304, 1981[ISI][Medline].

31.   Pettorossi, VE, Della Torre G, Bortolami R, and Brunetti O. The role of capsaicin-sensitive muscle afferents in fatigue-induced modulation of the monosynaptic reflex in the rat. J Physiol (Lond) 515: 599-607, 1999[Abstract/Free Full Text].

32.   Rall, JA. Energetic aspects of skeletal muscle contraction: implications of fiber types. In: Exercise and Sport Sciences Reviews, edited by Terjung RL.. New York: Macmillan, 1985, p. 33-74.

33.   Round, JM, Jones DA, Chapman SJ, Edwards RH, Ward PS, and Fodden DL. The anatomy and fibre type composition of the human adductor pollicis in relation to its contractile properties. J Neurol Sci 66: 163-172, 1984.

34.   Rowell, LB, Saltin B, Kiens B, and Juel Christensen N. Is peak quadriceps blood flow in humans even higher during exercise with hypoxemia? Am J Physiol Heart Circ Physiol 251: H1038-H1044, 1986[Abstract/Free Full Text].

35.   Sahlin, K, Tonkonogi M, and Soderlund K. Energy supply and muscle fatigue in humans. Acta Physiol Scand 162: 261-266, 1998[ISI][Medline].

36.   Semmler, JG, Kutzscher DV, and Enoka RM. Gender differences in the fatigability of human skeletal muscle. J Neurophysiol 82: 3590-3593, 1999[Abstract/Free Full Text].

37.   Simoneau, J-A, Lortie G, Boulay MR, Thibault MC, Thibault G, and Bouchard C. Skeletal muscle histochemical and biochemical characteristics in sedentary male and female subjects. Can J Appl Physiol 63: 30-35, 1985.

38.   Sinoway, LI, Shenberger J, Leaman G, Zelis R, Gray K, Baily R, and Lewenberger U. Forearm training attenuates sympathetic nerve responses to prolonged rhythmic forearm exercise. J Appl Physiol 81: 1778-1784, 1996[Abstract/Free Full Text].

39.   Sinoway, LI, Smith MB, Enders B, Leuenberger U, Dzwonczyk T, Gray K, Whisler S, and Moore RL. Role of diprotenated phosphate in evoking muscle reflex responses in cats and humans. Am J Physiol Heart Circ Physiol 267: H770-H778, 1994[Abstract/Free Full Text].

40.   Victor, RG, Bertocci LA, Pryor SL, and Nunnally RL. Sympathetic nerve discharge is coupled to muscle cell pH during exercise in humans. J Clin Invest 82: 1301-1305, 1988.

41.   Vollestad, KK, Sejersted OM, Bahr R, Woods JJ, and Bigland-Ritchie B. Motor drive and metabolic responses during repeated submaximal contractions in humans. J Appl Physiol 64: 1421-1427, 1988[Abstract/Free Full Text].

42.   Young, A, Wright J, Knapik J, and Cymerman A. Skeletal muscle strength during exposure to hypobaric hypoxia. Med Sci Sports Exerc 12: 330-335, 1980[ISI][Medline].

J APPL PHYSIOL 91(1):100-108


This article has been cited by other articles:


Home page
 Exp PhysiolHome page
R. A. Howlett and M. C. Hogan
Muscle: Effect of hypoxia on fatigue development in rat muscle composed of different fibre types
Exp Physiol, September 1, 2007; 92(5): 887 - 894.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. A. L. Calbet, G. Radegran, R. Boushel, H. Sondergaard, B. Saltin, and P. D. Wagner
Plasma volume expansion does not increase maximal cardiac output or VO2 max in lowlanders acclimatized to altitude
Am J Physiol Heart Circ Physiol, September 1, 2004; 287(3): H1214 - H1224.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. W. Russ and J. A. Kent-Braun
Sex differences in human skeletal muscle fatigue are eliminated under ischemic conditions
J Appl Physiol, June 1, 2003; 94(6): 2414 - 2422.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF) Free
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fulco, C. S.
Right arrow Articles by Lewis, S. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fulco, C. S.
Right arrow Articles by Lewis, S. F.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online