HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Free Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Search for Related Content

CORRIGENDUM

CORRIGENDUM

Pages 1930–1939: B. Yap and R. D. Kamm. Mechanical deformation of neutrophils into narrow channels induces pseudopod projection and changes in biomechanical properties. J Appl Physiol 98: 1930–1939, 2005; First published January 7, 2005; doi:10.1152/japplphysiol.01226.2004; http://jap.physiology.org/cgi/content/full/98/5/1930. An error in the particle-tracking algorithms used to track the Brownian motion of endogenous granules present in neutrophils caused the values of elastic modulus (G') and loss modulus (G'') reported in the article to be one order of magnitude too high. Specifically, the paragraph on page 1934 should read as follows: "To investigate the effects of mechanical deformation on the viscoelastic properties of neutrophils, we used the multiple-particle tracking method. The technique was first applied to adherent and spread neutrophils (Fig. 5B). Measurements at 1 Hz (see Table 1) revealed an elastic modulus, G', of 4.38 ± 0.5 dyn/cm², and viscous modulus, , of 0.22 ± 0.03 dyn·s·cm^–2. Round, passive neutrophils introduced into the glass-slide coverslip chamber and allowed to settle on the coverslip were also studied. Due to the presence of plasma in their surrounding medium, the majority of the neutrophils (98%) remained round (Fig. 5A) and free of pseudopod projection. Some of these adhered nonspecifically to the coverslip, whereas the rest floated loosely at the bottom of the chamber. Because the round, adherent cells remained stationary, they were chosen for particle tracking. The viscoelastic values of neutrophils were G' = 24.2 ± 2.1 dyn/cm² and G'' = 4.7 ± 4.0 dyn·cm² at 37°C, as summarized in Table 1. These values were significantly lower (P<0.05) than the viscoelastic moduli at 23°C (G'= 30.3 ± 1.9 dyn/cm² and G''= 64.9 ± 5.5 dyn·cm^–2), highlighting the effect of temperature on the mechanical properties of the cell."

View larger version (51K): [in this window] [in a new window]   Fig. 5. A: image of a round, passive neutrophil. Clearly seen are the endogenous granules in the cytoplasm of the cell. Scale bare, 5 µm. B: image of a neutrophil that had spread out on a coverslip showing many endogenous granules dispersed throughout the cytoplasm. As described in MATERIALS AND METHODS, only granules located at least 2 µm away from the nucleus (an example is shown with an arrow) were tracked, whereas granules situated proximal to the nucleus were ignored in the tracking. Scale bar, 5 µm. C: individual mean-squared-displacement (MSD) traces of randomly selected endogenous granules for passive, round neutrophils. Inset: typical trajectory of the centroid of a granule used to calculate the MSD. D: individual MSD traces of randomly selected endogenous granules for neutrophils that have undergone deformation into a microchannel. Inset: typical trajectory of the centroid of a granule used to calculate the MSD. E: typical plot of average MSD curves for neutrophils before and after exposure to mechanical stimulation.

Similarly, the reported values of G' and G'' in Table 1 are one order of magnitude too high. and the vertical axis of Figs. 5, C-E; 10; and 11 should read one order of magnitude lower. The corrected figures and table are reprinted correctly below.

View larger version (23K): [in this window] [in a new window]   Fig. 10. Temporal change in elastic modulus (G') of a neutrophil after mechanical deformation into a microchannel at a lag time of 0.03 s. Graphs show changes in G' under different flow conditions at 37°C. Time = 0 s represents the instance when the leading edge of the cell had just crossed the channel inlet. The time at which G' could first be recorded varied due to dissimilar entrance time for the different flow conditions. Values of G' for passive, round neutrophils at 37°C (Table 1) serve as control. Data are means ± SE. n, Number of cells; N, no. of granules. *P < 0.05 and **P < 0.01 compared with control; P < 0.05 and P <0.01 compared with data at time = 15 s.

View larger version (24K): [in this window] [in a new window]   Fig. 11. Temporal change in loss modulus (G'') of neutrophil after mechanical deformation into a microchannel at a lag time of 0.03 s. Graphs show changes in G'' under different flow conditions at 37°C. Time = 0 s represents the instance when the leading edge of the cell had just crossed the channel inlet. The time at which G'' could be first recorded varied due to dissimilar entrance time for the different flow conditions. Value of G'' for passive, round neutrophils at 37°C (Table 1) serves as control. Data are means ± SE. *P < 0.05 and **P < 0.01 compared with control; P < 0.05 and P <0.01 compared with data at time = 15 s.

This article has been cited by other articles:

				H. Oh, E. R. Mohler III, A. Tian, T. Baumgart, and S. L. Diamond Membrane Cholesterol Is a Biomechanical Regulator of Neutrophil Adhesion Arterioscler Thromb Vasc Biol, September 1, 2009; 29(9): 1290 - 1297. [Abstract] [Full Text] [PDF]

This Article Full Text (PDF) Free Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Search for Related Content