Journal of Applied Physiology Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 102: 1394-1401, 2007. First published January 4, 2007; doi:10.1152/japplphysiol.01254.2006
8750-7587/07 $8.00
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
102/4/1394    most recent
01254.2006v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baudry, S.
Right arrow Articles by Duchateau, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baudry, S.
Right arrow Articles by Duchateau, J.

Postactivation potentiation in a human muscle: effect on the rate of torque development of tetanic and voluntary isometric contractions

Stéphane Baudry and Jacques Duchateau

Laboratory of Applied Biology, Université Libre de Bruxelles, Brussels, Belgium

Submitted 6 November 2006 ; accepted in final form 2 January 2007


   ABSTRACT
TOP
 ABSTRACT
MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 ACKNOWLEDGMENTS
 REFERENCES
 
Postactivation potentiation (PAP), a mechanism by which the torque of a muscle twitch is increased following a conditioning contraction, is well documented in muscular physiology, but little is known about its effect on the maximal rate of torque development and functional significance during voluntary movements. The objective of this study was to investigate the PAP effect on the rate of isometric torque development of electrically induced and voluntary contractions. To that purpose, the electromechanical responses of the thumb adductor muscles to a single electrical stimulus (twitch), a train of 15 pulses at 250 Hz (HFT250), and during ballistic (i.e., rapid torque development) voluntary contractions at torque levels ranging from 10 to 75% of maximal voluntary contraction (MVC) were recorded before and after a conditioning 6-s MVC. The results showed that the rate of torque development was significantly (P < 0.001) increased after the conditioning MVC, but the effect was greater for the twitch (~200%) compared with the HFT250 (~17%) or ballistic contractions (range: 9–24%). Although twitch potentiation was maximal immediately after the conditioning MVC, maximal potentiation for HFT250 and ballistic contractions was delayed to 1 min after the 6-s MVC. Furthermore, the similar degree of potentiation for the rate of isometric torque development between tetanic and voluntary ballistic contractions indicates that PAP is not related to the modality of muscle activation. These observations suggest that PAP may be considered as a mechanism that can influence our contractions during daily tasks and can be utilized to improve muscle performance in explosive sports.

skeletal muscle; contractile properties; electrical stimulation

THE FORCE PRODUCED BY A MUSCLE twitch is history dependent, and its enhancement following a submaximal or maximal contraction has been referred to as postactivation potentiation (PAP; see Ref. 28). Twitch potentiation occurs both in mammalian (1, 16, 26, 33) and human muscles (2, 17, 27, 35) and is usually explained by the phosphorylation of myosin regulatory light chains (16, 26, 31, 38). This mechanism leads to a greater rate of cross-bridge attachment due to an increased sensitivity of the contractile proteins to ionized calcium (Ca2+) that subsequently increases twitch force and its rate of force development (24). PAP has been largely studied in response to single and trains of electrical stimuli in mammalian preparations (1, 16, 23), and its effect on the force appeared to be restricted to twitch and tetanic contractions at low frequency (1, 33). A similar trend has been observed in human muscle as the twitch potentiates to a greater extent than a brief high-frequency train of stimuli (2, 3). This lower PAP in the latter condition can be explained by a saturation process that limits the extent of potentiation of the successive responses within the train (2, 3). A ceiling effect is indeed observed for stimulation frequencies at 20 Hz or above and could, therefore, limit the impact of PAP on voluntary contractions (3).

Compared with electrically induced contractions, less is known about the effects of PAP on voluntary contractions (28). A limited number of studies have investigated the effects of PAP on fast voluntary contractions in humans. Some of them have reported an enhanced jump performance following strong conditioning contractions (11, 13, 37). French and coworkers (11) even observed an increase in torque production during a maximal isometric knee extension without change in the electromyogram (EMG) activity, suggesting that PAP occurred within the muscle. However, a weakness of these studies is that the presence of PAP was not assessed by the recording of twitch potentiation. It is, therefore, difficult to associate these improvements in performance to PAP, especially when the increase in mechanical output occurred ~20 min after the conditioning contraction (13), an elapsed time that is usually sufficient to abolish the PAP effect (2, 22, 35). In contrast, Gossen and Sale (15) did not find any velocity or power improvement during dynamic knee extension performed against various loads, at a time when twitch torque was significantly potentiated.

As suggested by the authors themselves, the lack of improvement of muscle performance in the study of Gossen and Sale (15) may be due to the relatively long (10 s) conditioning contraction that induced fatigue and thereby have counteracted the benefit derived from PAP. Furthermore, the results could have been influenced by the selected task that involves many different muscles. It is indeed possible that small changes in the coordination between synergist and antagonist muscles contributing to the knee extension could have influenced the mechanical output and suppressed any benefit from PAP. It was thus interesting to investigate whether PAP improves the performance of the muscular system during voluntary contraction by using a shorter conditioning contraction and a smaller muscle group, such as the adductor muscles of the thumb. An advantage of hand muscles is that tetanic contraction at maximal intensity is usually well tolerated by subjects. Therefore, the main purpose of the present study was to examine the effects of PAP and its decay over time on the maximal rate of torque development during ballistic isometric contractions, induced either voluntarily or by maximal electrical stimulation at high frequency.


   MATERIALS AND METHODS
TOP
 ABSTRACT
 MATERIALS AND METHODS
RESULTS
 DISCUSSION
 ACKNOWLEDGMENTS
 REFERENCES
 
After informed consent was obtained, experiments were conducted on 10 subjects (3 women and 7 men), aged between 24 and 40 yr (28.3 ± 4.7 yr; mean ± SD). None of them presented any signs of neurological disorders. Subjects were all right handed and instructed to refrain from any heavy arm exercise 24 h before testing. They attended the laboratory on two occasions: one session consisted of testing the effect of muscle potentiation on electrically induced tetanic contractions and maximal voluntary contraction (MVC); the second session assessed the effect of muscle potentiation on ballistic isometric voluntary contractions. In the latter condition, the task consisted of brief contractions in which the subject was instructed to reach the target torque as quickly as possible and without correction. The experimental procedure was approved by the local Ethics Committee and performed in accordance with the Helsinki Declaration.

Experimental Apparatus

The subject was seated in a comfortable armchair to achieve shoulder and arm relaxation throughout the experiment. The right hand was placed horizontally and securely held in the prone position by means of a custom-made apparatus. The thumb was maintained in full extension, in the same plane as the palm, by a splint that prevented movement at the phalangeal joints of the thumb. The splint was connected to a transducer (sensitivity: 0.27 V/N·m; linear range: 0–15 N·m) to measure the torque produced during the isometric contractions. All electrically induced and voluntary contractions were elicited at a thumb angle of 50° (0° = full adduction). This angle corresponds to the optimum thumb angle for maximal adduction torque (5).

EMG Recordings

The surface EMG from the adductor pollicis muscle was recorded by means of two silver disk electrodes (8 mm in diameter), separated by 1 cm and placed over the muscle belly. The ground electrode was located on the pisiform bone, between the stimulating and EMG recording electrodes. The EMG signal was amplified (1,000x) and filtered (10 Hz–1 kHz) by a custom-made differential amplifier. The torque and the EMG signals were recorded on a computer, at a sampling rate of 2 kHz, and analyzed off-line by using the AcqKnowledge data analysis software (model MP150; Biopac System, Santa Barbara, CA).

Stimulation Procedure

The adductor pollicis muscle was stimulated by rectangular electrical pulses (0.5 ms in duration) delivered through two electrodes (silver disks, 8 mm in diameter), placed over the ulnar nerve at the wrist. A digital timer (Master-8, AMPI, Jerusalem, Israel) was used to trigger the stimulator (Grass S88K, Astra-Med, West Warwick, RI). Maximal electrical stimulation was determined by progressively increasing the intensity until the compound muscle action potential (M-wave) and the mechanical twitch reached their maximal values. The level of stimulation was then set at ~20% above maximum.

Experimental Procedure

Protocol 1: High-frequency train of stimuli.   High-frequency trains of stimuli, consisting of 15 pulses delivered at a frequency of 250 Hz (HFT250), were used to induce contractions with the maximal rate of torque development in the adductor pollicis muscle (25). Before performing the conditioning MVC, we recorded the responses to three single twitches (twitchbefore) and one HFT250 followed after 5 s by one single twitch (twitchafter). This last stimulation was used to probe the possible potentiating effect of the testing contraction itself on the twitch (Fig. 1). Thereafter, the subject performed a 6-s conditioning MVC. Its duration was based on previous studies showing that maximum PAP occurred with maximal contractions of 5- to 10-s duration (27, 35). The tests carried out during the recovery period consisted of one single twitchbefore, one HFT250, and one single twitchafter, delivered in the following sequence: 5 s after the conditioning MVC, every min until 5 min, and after 10 min (Fig. 1A).


Figure 1
View larger version (15K):
[in this window]
[in a new window]

  		Fig. 1. Schematic representation of the two main protocols: high-frequency train of stimuli (A) and ballistic contractions (B). Twitchbefore and twitchafter are the mechanical responses to a single maximal electrical stimulus before and after, respectively, a short high-frequency (250 Hz) train of electrical stimuli (HFT250) or a ballistic contraction at 10, 20, 50 or 75% MVC. 6-s MVC corresponds to a 6-s conditioning maximal voluntary contraction.

 
The HFT250 did not evoke a maximal tetanic torque plateau and consequently did not allow the investigation of the effect of PAP on the maximal tetanic torque. To address this issue, additional experiments were conducted in all subjects, during which a contraction consisting of 50 pulses at 100-Hz frequency (HFT100) was substituted for the HFT250 before and after the 6-s MVC. Furthermore, during the same session, additional experiments were carried out to assess the possible fatigue effect induced by the 6-s MVC on the subsequent recordings, and the subject produced a 3-s MVC before and after the conditioning MVC at a timing that was similar to the general experimental protocol, except that twitches were not induced. These last two experiments were performed in a random manner.

Protocol 2: Ballistic voluntary contraction.   The experiment began with the recording of three MVCs separated by 2-min intervals. The largest MVC torque was taken as the maximal voluntary torque and served to calculate the target levels used in the various ballistic protocols (see below). The target torque and the actual torque produced were displayed on an oscilloscope in front of the subject. For ~10 min, subjects performed ballistic contractions at four different target levels (10, 20, 50, and 75% of MVC). To minimize any possible fatigue effect induced by this familiarization procedure, subjects were instructed to perform several sets of ~15 contractions with an interval of 3–5 s between contractions, with each set being separated by 1 min of rest. After the familiarization program, subjects rested during the placement of the stimulating and recording electrodes. This procedure lasted ~20 min, an elapsed time sufficient to abolish any potentiating effect induced by the previous contractions (2, 27, 35). Thereafter, subjects performed four distinct protocols in random order at the four previously reported target levels. The testing protocol began with the recording of three twitches (twitchbefore), five ballistic contractions reaching one of the four target levels, and followed 5 s later by a single twitch (twitchafter; Fig. 1B). After these control recordings, subjects performed the conditioning 6-s MVC, followed by one twitchbefore, five ballistic contractions, and one twitchafter. The tests during the recovery period were carried out with the same timing used for the high-frequency train protocol. To ensure that twitch parameters recovered their control values before the beginning of the next target force protocol, a minimum of 10-min rest period was given. Three twitches were elicited and measured, and the subsequent protocol began only if twitch amplitude did not differ by >5% from the initial control values.

Measurements

Electrically induced contractions.   The peak torque of the twitch (Pt-before and Pt-after) and tetanus (PT) in response to HFT250, as well as the twitch contraction time (CT) and one-half relaxation time (RT1/2), were measured. The maximal rate of torque development (+dPt/dt or +dPT/dt) and relaxation (–dPt/dt or –dPT/dt) were obtained from the first derivative of the torque signal. The PAP effects on the twitch and HFT250 were measured and expressed as percentage of the control values recorded before the 6-s MVC. The potentiating effect of HFT250 or ballistic contractions, used to probe the extent of PAP on the maximal rate of torque development on the twitchafter were expressed as percentage of the twitch elicited before (twitchbefore) the HFT250 or ballistic contractions. For electrically induced contractions, the M-wave peak-to-peak amplitude was measured from the EMG signal.

Voluntary contractions.   The average torque value during the MVCs and the associated averaged (rectified) EMG (aEMG) were measured during a 1-s epoch at the torque plateau. The peak torque and maximal rate of torque development computed by the first derivative of each ballistic contraction were measured. The aEMG activity was analyzed from its onset to the time at which the peak rate of torque development was reached. Because the ballistic contractions did not reach precisely the different target levels, we calculated the relation between the peak rate of torque development (expressed as %MVC/ms) and torque achieved during the ballistic contraction (expressed as %MVC) for each subject. As this relation was linear (r2 >0.96), we used this linear relation for each subject to determine the PAP effect on the rate of torque development associated with the fixed target levels: 10, 20, 50, and 75% MVC.

Statistical Analysis

In protocol 1, the effects of PAP induced by the conditioning 6-s MVC or HFT250 were analyzed by a one-way ANOVA with repeated measures over time. A Dunnett post hoc test was used to identify the significant differences among the selected means when the ANOVA reached a significant value. The effect of PAP on the HFT100 and 3-s MVCs was analyzed by a two-way ANOVA (contraction type x time). In protocol 2, the effect of PAP induced by the conditioning 6-s MVC or ballistic contractions was analyzed by a two-way ANOVA (torque level x time) with repeated measures on both factors. In the last two analyses, a Tukey post hoc test was used to identify the significant differences among the selected means. The linear regressions between torque and rate of torque development for the ballistic contractions were compared by a repeated-measures analysis of covariance (rate of torque development x time, with torque level as covariate) and Dunnett post hoc test. For all comparisons, the level of statistical significance was set at P < 0.05. Data are reported as means ± SD within the text and displayed as means ± SE in Figs. 2, 3, 5, 6, and 7.


Figure 2
View larger version (16K):
[in this window]
[in a new window]

  		Fig. 2. Time course of postactivation potentiation for peak torque of twitchbefore (Pt-before; A) and its first derivative (+dPt/dtbefore; B) during HFT250 ({circ}) and ballistic (bullet) protocols. Data from the protocol using voluntary contractions have been collapsed across intensities. In inset, twitch torque traces before and 5 s after a 6-s MVC from one subject are superimposed. Values are means ± SE; n = 10 subjects. *Significant difference (P < 0.01) with control values.

 

Figure 3
View larger version (17K):
[in this window]
[in a new window]

  		Fig. 3. Time course of postactivation potentiation for peak torque of twitchafter (Pt-after; A) and its first derivative (+dPt/dtafter; B; both expressed as percentage of the twitchbefore) during HFT250 ({circ}) and ballistic contractions at 75% MVC (bullet) protocols. CON, control. Values are means ± SE; n = 10 subjects. Significant difference (P < 0.01) with control values for {dagger}HFT250 and *voluntary protocols.

 

Figure 4
View larger version (17K):
[in this window]
[in a new window]

  		Fig. 4. Typical recordings from one subject before and 1 min after the conditioning 6-s MVC during maximal tetanic contraction at 250-Hz frequency (A) and ballistic voluntary contractions (B) of matched torque. Traces corresponds successively to muscle torque (a), corresponding first derivative (b), and electromyographic activity before (c) and after the conditioning MVC (d). The arrows indicate mechanical traces recorded after the conditioning MVC. Note that, although the torque traces look like twitch traces, they are from repetitive (electrically induced or voluntary) activation of short duration.

 

Figure 5
View larger version (14K):
[in this window]
[in a new window]

  		Fig. 5. Time course of potentiation of a 6-s conditioning MVC on the torque [peak torque of tetanus (PT); A] and its maximal rate of development (+dPT/dt; B) of a tetanic contraction at 250 Hz (HFT250). Values are means ± SE expressed as % of control values; n = 10. *Significant difference (P < 0.01) with control values.

 

Figure 6
View larger version (17K):
[in this window]
[in a new window]

  		Fig. 6. Time course of potentiation of a 6-s conditioning MVC on the rate of torque development of ballistic voluntary contractions performed at torque levels of 10, 20, 50, and 75% MVC. Values are means ± SE expressed as % of control values; n = 10. Significant difference (P < 0.05) with *control values and {dagger}data obtained at 10% MVC.

 

Figure 7
View larger version (13K):
[in this window]
[in a new window]

  		Fig. 7. Relation between the degree of potentiation of the rate of torque development and the torque level (expressed as % of MVC) reached during the ballistic voluntary contractions. Data are fitted by a one-phase exponential decay equation: Y = 25.01–0.0472x + 108.6 (r2 = 0.99). Values are means ± SE expressed as % of control values; n = 10.

 

   RESULTS
TOP
 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
DISCUSSION
 ACKNOWLEDGMENTS
 REFERENCES
 
Reproducibility of the Conditioning MVC

During the various experimental protocols, each subject had to perform a total of seven conditioning MVCs. Because the MVC torque and associated aEMG did not differ from trial to trial (P = 0.50 and 0.78, respectively), data were collapsed across contractions. The average MVC torque produced by the thumb adductor muscles was 10.3 ± 3.9 N·m, and the aEMG activity was 168.2 ± 136.3 µV.

PAP and Muscle Twitch

The mean characteristics of the twitch before the conditioning 6-s MVC (twitchbefore) are reported in Table 1. Before the conditioning contraction, no significant difference (ANOVA; P > 0.05) was found between the parameters of the twitchbefore recorded in the two protocols (HFT250 and ballistic contractions). Immediately after the conditioning MVC, Pt-before, and its rate of torque development (+dPt/dtbefore), and of relaxation (–dPt/dtbefore), recorded during the HFT250 protocol, were potentiated and reached 280.0 ± 67.8, 297.1 ± 75.8, and 311.6 ± 84.9% of control values, respectively (Dunnett post hoc test; P < 0.001). For the ballistic contractions protocol, there was no significant difference in the extent of PAP (torque level x time: P > 0.05), and, therefore, data were collapsed across intensities. The average potentiation of Pt-before, +dPt/dtbefore, and –dPt/dtbefore recorded immediately after the conditioning MVC reached 254.4 ± 78.8, 281.6 ± 97.8, and 287.9 ± 90.8% of control values, respectively (Dunnett post hoc test; P < 0.001). There was no significant difference in potentiation for these parameters between HFT250 and ballistic contraction protocols (ANOVA; P > 0.05). For both protocols, potentiation was maximal immediately after the conditioning MVC, declined rapidly during the 1st min of the recovery period and then more slowly, to return to control values within 10 min (Fig. 2). In contrast, CT, RT1/2, and M-wave amplitude were not affected by the conditioning MVC (P > 0.05).


View this table:
[in this window]
[in a new window]

  		Table 1. Mechanical parameters for the twitchbefore, tetanic contraction at 250 Hz, and ballistic voluntary contractions for different target levels before the conditioning MVC

 
The muscle twitches (twitchafter) that followed each HFT250 and ballistic contractions were also affected by this previous activity. In the control conditions, Pt-after reached 133.3 ± 5.4% of the Pt-before, after the HFT250 (Dunnett post hoc test, P < 0.01). A similar finding was observed after the ballistic voluntary contractions. However, PAP extent of the twitchafter was greater (Tukey post hoc test; P < 0.01) for contractions at 75% MVC (149.7 ± 32.3%; P < 0.01) compared with that at 50% (118.1 ± 8.1%; P < 0.01), 20% (118.4 ± 22.2%; P < 0.01), and 10% MVC (107.3 ± 11.1%; P > 0.05; Fig. 3). Following the 6-s MVC, the extent of twitchafter potentiation induced by the HFT250 and ballistic contractions at 75% MVC dropped to 91.2 ± 8.1% (P < 0.01) and 89.2 ± 12.5% (P < 0.01) of Pt-before, respectively, with similar patterns for ballistic contractions at lower target levels. Except for ballistic contractions at 75% MVC (Fig. 3) that remained reduced until the 5th min of the recovery period (Tukey post hoc test; P < 0.001), the potentiating effect of HFT250 and ballistic contractions returned to their initial values (Dunnett and Tukey post hoc tests; P > 0.05) within 1 min after the conditioning MVC.

PAP and HFT250

Figure 4A illustrates the mechanical and electrical responses to a 15-pulse, 250-Hz train before and 1 min after the 6-s MVC. Before the conditioning MVC, the average torque was 7.0 ± 1.8 N·m (~68% MVC), and the associated rate of torque development reached 107.8 ± 9.3 N·m·s–1 (Table 1). Immediately after the conditioning MVC, the tetanic torque did not change significantly, whereas 1 min later, the torque increased to 112.5 ± 6.6% of the control value (Dunnett post hoc test; P < 0.01) and remained potentiated until 5 min after the MVC (Fig. 5A). The peak rate of torque development increased with a similar pattern, except that 5 s after the conditioning MVC, +dPT/dt was already enhanced to 112.2 ± 3.4% (Dunnett post hoc test; P < 0.01; Fig. 5B) but reached its maximal value (117.4 ± 10.3%) 1 min after the MVC. In contrast, no change was observed for –dPT/dt (ANOVA; P > 0.05).

PAP and Ballistic Voluntary Contractions

For each subject, the peak rate of torque development was linearly related to peak torque achieved during the ballistic contractions (r2 between 0.96 and 0.99). PAP increased the rate of torque development (see Fig. 4B, analysis of covariance, P < 0.001), and Dunnett post hoc test revealed significant differences between the linear regressions recorded before and 5 s (P < 0.05), 1 min (P < 0.01), and 2 min (P < 0.01) after the conditioning MVC, with the greatest effect being observed at 1 min post-MVC. Regardless of the protocol, the aEMG activity did not change throughout the recovery period (ANOVA; P > 0.05).

Figure 6 illustrates the effect of PAP on the peak rate of torque development during voluntary ballistic contractions extrapolated from the individual torque rate of torque development relations. Similar to what was observed for the HFT250, the peak rate of torque development of ballistic contractions was significantly potentiated immediately after the MVC and, regardless of the target level, reached its maximal value 1 min after the conditioning MVC. A greater potentiation (torque level x time; P < 0.05) was obtained at 10% MVC (124.3 ± 17.2% of control value) compared with those at 50% (111.3 ± 4.1) and 75% (109.1 ± 4.2) MVC (Tukey post hoc test; P < 0.05 and P < 0.01, respectively). The extent of potentiation decreased exponentially as the torque attained during the ballistic contraction increased (Fig. 7). In addition, the rate of torque development for ballistic contractions at 10 and 20% MVC was still potentiated 2 min after the conditioning MVC (Tukey post hoc test; P < 0.01), whereas those for contractions at 50 and 75% MVC were only significantly (P < 0.05) potentiated up to 1 min after the conditioning MVC (Tukey post hoc test; P < 0.05).

PAP and MVC or HFT100

In control condition, the average maximal torque developed in response to a 50-pulse 100 Hz was 7.8 ± 2.1 N·m and corresponded to ~73% of the 3-s MVC torque (10.7 ± 2.3 N·m). This MVC torque value did not differ from the torque recorded during the 6-s MVC. Immediately after the conditioning 6-s MVC, the torque developed during the HFT100 and the 3-s MVC was reduced (time effect; P < 0.001) to 92.3 ± 3.8 and 88.6 ± 4.3%, respectively (Dunnett post hoc test; P < 0.01). These changes were transient since, 1 min later, the torque of both contraction types returned to control values. No potentiation of these contractions was observed throughout the recovery period.


   DISCUSSION
TOP
 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
ACKNOWLEDGMENTS
 REFERENCES
 
To investigate the effect of a conditioning MVC on the peak rate of torque development of a subsequent contraction, we recorded the mechanical responses to a single stimulus (twitch), HFT250, and ballistic voluntary contractions at different torque levels. Our results show that 1) the increase of the rate of torque development was larger for the twitch (~200%) compared with HFT250 (~17%) or ballistic contractions (from 9 to 24% for torque levels ranging from 75 to 10% MVC); 2) twitch potentiation was maximal immediately after the conditioning MVC, whereas the rate of torque development for HFT250 and ballistic contractions was maximally potentiated 1 min post-MVC; and 3) whereas twitch potentiation declined nearly exponentially over time to disappear within the following 10 min of recovery, the rate of torque development for HFT250 and ballistic contractions declined more progressively and remained significantly potentiated during 5 and ~2 min, respectively. Although relatively small compared with the twitch, the similar extent of potentiation for the rate of torque development between electrically induced (HFT250) and ballistic voluntary contractions indicates that PAP is not related to the modality of muscle activation. To our knowledge, this is the first detailed study that reports an increased maximal rate of torque development of voluntary contractions in the presence of twitch potentiation.

PAP and Muscle Twitch

Muscle twitch was recorded and analyzed because it represents the most common tool to establish the presence of PAP. In the present study, twitch torque recorded after the conditioning MVC (twitchbefore) and its peak rate of torque development and of relaxation were significantly increased during 5 min following the conditioning MVC, the greatest effect being obtained immediately after the MVC. These results are in agreement with those of previous studies that reported a similar degree of PAP and decay over time (2, 3, 17, 30, 35). In our study, the time course of the potentiated twitch did not differ from the control twitch, whereas it was shown to be shortened in some previous studies (17, 27). Although we do not have a clear explanation for these contrasting results, it must be mentioned that other studies did not observe changes in twitch CT and RT1/2 after a conditioning contraction (2, 3, 35). Regardless, the causes of this divergence between studies, the observation that M-wave peak-to-peak amplitude was unchanged during PAP, confirms that potentiation is mainly related to intramuscular mechanisms (2, 3, 24, 26).

In control conditions, the twitch (twitchafter) recorded after each HFT250 and ballistic contractions was used to probe the possible potentiating effect of the testing contraction itself on the twitch. Our results shows that the size of twitchafter was also increased by these previous contractions. Such twitch enhancement, following low- or high-frequency trains of stimuli, has already been reported in mammalian (1, 16) and human muscles for various frequencies and durations of stimulation (4, 7). However, the potentiation of the twitchafter observed after the ballistic contractions performed at intensities as low as 20% of MVC was unexpected, because Vandervoort and coworkers (35) reported that brief, voluntary isometric contractions below 75% MVC produced little or no potentiation. Our observation that ballistic contractions at 20% of MVC induced twitch potentiation could be related to the involvement of a greater number of motor units during fast voluntary contractions compared with sustained submaximal contractions performed at similar intensities (6, 9). This enhanced motor unit recruitment involves higher force-threshold motor units (comprised of faster twitch fibers) that display greater PAP capacity than lower threshold motor units (14, 27). This potentiating effect of the testing contraction may explain why Pt-before did not follow a strict exponential decline during the recovery period in the present study (Fig. 2), as is the case when PAP decay is tested by single twitches only (2, 3, 17, 30). Indeed, each short train of electrical stimuli (HFT250) and the five ballistic contractions could have contributed to maintaining potentiation at a higher level and partly affected the normal PAP decay.

PAP and Rate of Torque Development

The most important result of this study is the potentiating effect of a 6-s MVC on the maximal rate of torque development during ballistic voluntary contractions. This finding contrasts with the lack of increase in knee extension velocity after PAP in the study of Gossen and Sale (15). The shorter conditioning contraction in our study (6 vs. 10 s) and the greater percentage of fatigue-resistant fibers in the adductor pollicis compared with the quadriceps (19) may have reduced the counteracting effects of fatigue on the benefit derived from PAP in our experimental conditions. However, our results are in agreement with results from mammalian models showing an enhanced maximal rate of isometric force development (34) and an upward shift of the load-velocity relation (16) after a 5-Hz, 20-s conditioning contraction. Furthermore, our results indicate that PAP was greater for contractions at low-torque levels, since the ballistic contractions at 10% MVC exhibited a greater potentiation of the peak rate of torque development compared with contractions at 50 and 75% of MVC. This original observation indicates that the potentiating effect on the rate of torque development is related to the torque achieved during the ballistic contraction (Fig. 7).

It was previously shown in the human tibialis anterior that the extent of PAP on the successive responses of an electrical train of stimuli declined with increased frequency of stimulation (3). Furthermore, Desmedt and Godaux (6) reported that motor unit discharge rate during ballistic isometric contractions increases with the torque reached. These observations might partly account for the slightly greater PAP effect on ballistic contractions at 10% MVC compared with ballistic contractions of higher torque levels or HFT250 (Figs. 5 and 7), since a lower motor unit discharge rate would magnify the effect of potentiation on the summation of the successive contractions compared with higher frequencies. Therefore, PAP appears to be more effective during ballistic contractions at low- than high-torque levels for the thumb adductor muscles. Because potentiation is greater for high-threshold compared with low-threshold motor units (14, 29), the greater PAP effect for ballistic contractions of low-torque level could be surprising at first. However, it has been shown that most motor units are recruited at a ~33% maximal torque during a ballistic contraction in the tibialis anterior (6). As the adductor pollicis displays a narrower range of recruitment than the tibialis anterior during slow contractions (8, 32), one can expect that most motor units are recruited below a torque level of ~20% during ballistic contractions. A clear understanding of PAP modulation during ballistic voluntary contractions requires further studies in relation to motor unit recruitment and rate coding.

PAP Time Course

Twitch torque and its peak rate of torque development (twitchbefore) were maximally potentiated immediately after the conditioning MVC. In contrast, for both HFT250 and ballistic contractions, maximal potentiation of their rate of torque development occurred 1 min after the conditioning MVC (Figs. 5 and 6). This delayed effect could be explained by a saturation process that limits the extent of potentiation on the summation of contractions immediately after the conditioning contraction (2, 3, 7). For example, recent studies (2, 3) reported that the contribution of the third pulse in a three-pulse train (100 Hz) was depressed immediately after a 6-s MVC but slightly potentiated from the 1st to the 4th min of the recovery period. This observation suggests a ceiling effect, probably linked to the level of free cytosolic Ca2+ concentration immediately after the conditioning contraction (24). In the present study, this ceiling effect could have contributed to delay the increase of the torque and its rate of development by limiting the potentiation of the successive muscle activations within a HFT250 or a ballistic contraction performed immediately after the 6-s MVC. Moreover, this saturation effect may explain the delayed potentiation of the torque compared with the rate of torque development, because the greatest effect of myosin regulatory light-chain phosphorylation on isometric torque potentiation is obtained at low Ca2+ activation level, whereas, for the rate of torque development, it is reached at higher Ca2+ concentration (24).

The above discussion of a possible ceiling effect cannot, however, account entirely for the decrease of Pt-after compared with Pt-before after the 6-s MVC, because at that time the tetanic and MVC torques are also reduced. It could, therefore, be hypothesized that some other mechanisms may have interfered with PAP during the few seconds that follow the conditioning MVC. The fact that PAP may coexist with fatigue, the former delaying the latter (10, 12, 18), and the reduced torque recorded in response to the HFT100 and 3-s MVC immediately after the conditioning 6-s MVC in the present study, suggest that fatigue could have also contributed to reduce the extent of potentiation. This proposal is in agreement with a previous study showing a substantial reduction in torque output after a 10-s MVC in the adductor pollicis muscle (21). This loss in MVC torque was associated with a reduction of the torque produced in response to a 3-s tetanic contraction at 80 Hz, whereas no impairment was found in response to a tetanic contraction of similar duration at 20 Hz. Because high-frequency fatigue is transient (20, 36), it could partly explain the reduced MVC and twitch (Pt-after) torque immediately after the conditioning MVC, as well as the delayed maximal PAP observed for HFT250 and ballistic contractions. Regardless of the underlying mechanisms, our results indicate that the extent and time course of PAP are different for the twitch and HFT250 or ballistic contractions.

In conclusion, the main finding of the present study is the significant enhancement of the rate of torque development of tetanic and ballistic voluntary contractions associated with PAP. Although twitch potentiation is maximal immediately after the conditioning MVC, the rate of torque development of electrically induced and ballistic voluntary contractions is maximally enhanced 1 min after the MVC and remained significantly potentiated during, respectively, 5 and 2 min. These findings suggest that PAP may be considered as a mechanism that can influence our contractions during daily tasks and can be utilized to improve muscle performance in explosive sports.


   ACKNOWLEDGMENTS
TOP
 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 ACKNOWLEDGMENTS
REFERENCES
 
The authors are particularly grateful to Dr S. J. Garland (University of Western Ontario) for helpful comments on a draft of this manuscript.


   FOOTNOTES
 

Address for reprint requests and other correspondence: J. Duchateau, Laboratory of Applied Biology, Université Libre de Bruxelles, 28 Ave. P. Héger, CP 168, 1000 Brussels, Belgium (e-mail: jduchat{at}ulb.ac.be)

The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.


   REFERENCES
TOP
 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 ACKNOWLEDGMENTS
 REFERENCES
 

  1. Abbate F, Sargeant AJ, Verdijk PW, De Haan A. Effects of high-frequency initial pulses and posttetanic potentiation on power output of skeletal muscle. J Appl Physiol 88: 35–40, 2000.[Abstract/Free Full Text]
  2. Baudry S, Duchateau J. Post-activation potentiation in human muscle is not related to the type of maximal conditioning contraction. Muscle Nerve 30: 328–336, 2004.[CrossRef][Web of Science][Medline]
  3. Baudry S, Klass M, Duchateau J. Postactivation potentiation influences differently the nonlinear summation of contractions in young and elderly adults. J Appl Physiol 98: 1243–1250, 2005.[Abstract/Free Full Text]
  4. Binder-MacLeod SA, Dean JC, Ding J. Electrical stimulation factors in potentiation of human quadriceps femoris. Muscle Nerve 25: 271–279, 2002.[CrossRef][Web of Science][Medline]
  5. De Ruiter CJ, De Haan A, Jones DA, Sargeant AJ. Shortening-induced force depression in human adductor pollicis muscle. J Physiol 507: 583–591, 1998.[Abstract/Free Full Text]
  6. Desmedt JE, Godaux E. Ballistic contractions in man: characteristic recruitment pattern of single motor units of the tibialis anterior muscle. J Physiol 264: 673–693, 1977.[Abstract/Free Full Text]
  7. Ding J, Storaska JA, Binder-MacLeod SA. Effect of potentiation on the catchlike property of human skeletal muscles. Muscle Nerve 27: 312–319, 2003.[CrossRef][Web of Science][Medline]
  8. Duchateau J, Hainaut K. Effects of immobilization on contractile properties, recruitment and firing rates of human motor units. J Physiol 422: 55–65, 1990.[Abstract/Free Full Text]
  9. Duchateau J, Semmler JG, Enoka RM. Training adaptations in the behavior of human motor units. J Appl Physiol 101: 1766–1775, 2006.[Abstract/Free Full Text]
  10. Fowles JR, Green HJ. Coexistence of potentiation and low-frequency fatigue during voluntary exercise in human skeletal muscle. Can J Physiol Pharmacol 81: 1092–1100, 2003.[CrossRef][Web of Science][Medline]
  11. French DN, Kraemer WJ, Cooke CB. Changes in dynamic exercise performance following a sequence of preconditioning isometric muscle actions. J Strength Cond Res 17: 678–685, 2003.[CrossRef][Web of Science][Medline]
  12. Garner SH, Hicks AL, McComas AJ. Prolongation of twitch potentiating mechanism throughout muscle fatigue and recovery. Exp Neurol 103: 277–281, 1989.[Web of Science][Medline]
  13. Gilbert G, Lees A. Changes in the force development characteristics of muscle following repeated maximum force and power exercise. Ergonomics 48: 1576–1584, 2005.[Medline]
  14. Gordon DA, Enoka RM, Stuart DG. Motor-unit force potentiation in adult cats during a standard fatigue test. J Physiol 421: 569–582, 1990.[Abstract/Free Full Text]
  15. Gossen ER, Sale DG. Effect of postactivation potentiation on dynamic knee extension performance. Eur J Appl Physiol 83: 524–530, 2000.[CrossRef][Web of Science][Medline]
  16. Grange RW, Cory CR, Vandenboom R, Houston ME. Myosin phosphorylation augments force-displacement and force-velocity relationships of mouse fast muscle. Am J Physiol Cell Physiol 269: C713–C724, 1995.[Abstract/Free Full Text]
  17. Hamada T, Sale DG, MacDougall JD, Tarnopolsky MA. Postactivation potentiation, fiber type, and twitch contraction time in human knee extensor muscles. J Appl Physiol 88: 2131–2137, 2000.[Abstract/Free Full Text]
  18. Hicks AL, Cupido CM, Martin J, Dent J. Twitch potentiation during fatiguing exercise in the elderly: the effect of training. Eur J Appl Physiol 63: 278–281, 1991.[CrossRef][Web of Science]
  19. Johnson MA, Polgar J, Weightman D, Appleton D. Data on the distribution of fibre types in thirty-six human muscles. An autopsy study. J Neurol Sci 18: 111–29, 1973.[CrossRef][Web of Science][Medline]
  20. Jones DA. High- and low-frequency fatigue revisited. Acta Physiol Scand 156: 265–270, 1996.[CrossRef][Web of Science][Medline]
  21. Jones DA, Bigland-Ritchie B, Edwards RH. Excitation frequency and muscle fatigue: mechanical responses during voluntary and stimulated contractions. Exp Neurol 64: 401–413, 1979.[CrossRef][Web of Science][Medline]
  22. Klein CS, Ivanova TD, Rice CL, Garland SJ. Motor unit discharge rate following twitch potentiation in human triceps brachii muscle. Neurosci Lett 316: 153–156, 2001.[CrossRef][Web of Science][Medline]
  23. MacIntosh BR, Willis JC. Force-frequency relationship and potentiation in mammalian skeletal muscle. J Appl Physiol 88: 2088–2096, 2000.[Abstract/Free Full Text]
  24. Metzger JM, Greaser ML, Moss RL. Variations in cross-bridge attachment rate and tension with phosphorylation of myosin in mammalian skinned skeletal muscle fibers. Implications for twitch potentiation in intact muscle. J Gen Physiol 93: 855–883, 1989.[Abstract/Free Full Text]
  25. Miller RG, Mirka A, Maxfield M. Rate of tension development in isometric contractions of a human hand muscle. Exp Neurol 73: 267–285, 1981.[CrossRef][Web of Science][Medline]
  26. Moore RL, Stull JT. Myosin light chain phosphorylation in fast and slow skeletal muscles in situ. Am J Physiol Cell Physiol 247: C462–C471, 1984.[Abstract/Free Full Text]
  27. O'Leary DD, Hope K, Sale DG. Posttetanic potentiation of human dorsiflexors. J Appl Physiol 83: 2131–2138, 1997.[Abstract/Free Full Text]
  28. Sale DG. Postactivation potentiation: role in human performance. Exerc Sport Sci Rev 30: 138–143, 2002.[CrossRef][Web of Science][Medline]
  29. Stephens JA, Stuart DG. The motor units of cat medial gastrocnemius. Twitch potentiation and twitch-tetanus ratio. Pflügers Arch 356: 359–372, 1975.[CrossRef][Web of Science][Medline]
  30. Stuart DS, Lingley MD, Grange RW, Houston ME. Myosin light chain phosphorylation and contractile performance of human skeletal muscle. Can J Physiol Pharmacol 66: 49–54, 1988.[Web of Science][Medline]
  31. Sweeney HL, Bowman BF, Stull JT. Myosin light chain phosphorylation in vertebrate striated muscle: regulation and function. Am J Physiol Cell Physiol 264: C1085–C1095, 1993.[Abstract/Free Full Text]
  32. Van Cutsem M, Feiereisen P, Duchateau J, Hainaut K. Mechanical properties and behaviour of motor units in the tibialis anterior during voluntary contractions. Can J Appl Physiol 22: 585–597, 1997.[Web of Science][Medline]
  33. Vandenboom R, Grange RW, Houston ME. Threshold for force potentiation associated with skeletal myosin phosphorylation. Am J Physiol Cell Physiol 265: C1456–C1462, 1993.[Abstract/Free Full Text]
  34. Vandenboom R, Grange RW, Houston ME. Myosin phosphorylation enhances rate of force development in fast-twitch skeletal muscle. Am J Physiol Cell Physiol 268: C596–C603, 1995.[Abstract/Free Full Text]
  35. Vandervoort AA, Quinlan J, McComas AJ. Twitch potentiation after voluntary contraction. Exp Neurol 81: 141–152, 1983.[CrossRef][Web of Science][Medline]
  36. Westerblad H, Allen DG. Changes of myoplasmic calcium concentration during fatigue in single mouse muscle fibers. J Gen Physiol 98: 615–635, 1991.[Abstract/Free Full Text]
  37. Young WB, Behm DG. Effects of running, static stretching and practice jumps on explosive force production and jumping performance. J Sports Med Phys Fitness 43: 21–27, 2003.[Web of Science][Medline]
  38. Zhi G, Ryder JW, Huang J, Ding P, Chen Y, Zhao Y, Kamm KE, Stull JT. Myosin light chain kinase and myosin phosphorylation effect frequency-dependent potentiation of skeletal muscle contraction. Proc Natl Acad Sci USA 102: 17519–17524, 2005.[Abstract/Free Full Text]



This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
A. D. Kay and A. J. Blazevich
Isometric contractions reduce plantar flexor moment, Achilles tendon stiffness, and neuromuscular activity but remove the subsequent effects of stretch
J Appl Physiol, October 1, 2009; 107(4): 1181 - 1189.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. G. Allen, G. D. Lamb, and H. Westerblad
Impaired calcium release during fatigue
J Appl Physiol, January 1, 2008; 104(1): 296 - 305.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. Baudry and J. Duchateau
Postactivation potentiation in a human muscle: effect on the load-velocity relation of tetanic and voluntary shortening contractions
J Appl Physiol, October 1, 2007; 103(4): 1318 - 1325.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. W. Ryder, K. S. Lau, K. E. Kamm, and J. T. Stull
Enhanced Skeletal Muscle Contraction with Myosin Light Chain Phosphorylation by a Calmodulin-sensing Kinase
J. Biol. Chem., July 13, 2007; 282(28): 20447 - 20454.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
102/4/1394    most recent
01254.2006v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baudry, S.
Right arrow Articles by Duchateau, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baudry, S.
Right arrow Articles by Duchateau, J.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2007 by the American Physiological Society.