Journal of Applied Physiology Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 102: 547-556, 2007. First published September 28, 2006; doi:10.1152/japplphysiol.00282.2006
8750-7587/07 $8.00
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
102/2/547    most recent
00282.2006v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (6)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Patil, S. P.
Right arrow Articles by Smith, P. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Patil, S. P.
Right arrow Articles by Smith, P. L.

Neuromechanical control of upper airway patency during sleep

Susheel P. Patil, Hartmut Schneider, Jason J. Marx, Elizabeth Gladmon, Alan R. Schwartz, and Philip L. Smith

Department of Medicine, Division of Pulmonary and Critical Care Medicine, Johns Hopkins University, Baltimore, Maryland

Submitted 6 March 2006 ; accepted in final form 26 September 2006


   ABSTRACT
TOP
 ABSTRACT
MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 GRANTS
 ACKNOWLEDGMENTS
 REFERENCES
 
Obstructive sleep apnea is caused by pharyngeal occlusion due to alterations in upper airway mechanical properties and/or disturbances in neuromuscular control. The objective of the study was to determine the relative contribution of mechanical loads and dynamic neuromuscular responses to pharyngeal collapse during sleep. Sixteen obstructive sleep apnea patients and sixteen normal subjects were matched on age, sex, and body mass index. Pharyngeal collapsibility, defined by the critical pressure, was measured during sleep. The critical pressure was partitioned between its passive mechanical properties (passive critical pressure) and active dynamic responses to upper airway obstruction (active critical pressure). Compared with normal subjects, sleep apnea patients demonstrated elevated mechanical loads as demonstrated by higher passive critical pressures [–0.05 (SD 2.4) vs. –4.5 cmH2O (SD 3.0), P = 0.0003]. Dynamic responses were depressed in sleep apnea patients, as suggested by failure to lower their active critical pressures [–1.6 (SD 3.5) vs. –11.1 cmH2O (SD 5.3), P < 0.0001] in response to upper airway obstruction. Moreover, elevated mechanical loads placed some normal individuals at risk for sleep apnea. In this subset, dynamic responses to upper airway obstruction compensated for mechanical loads and maintained airway patency by lowering the active critical pressure. The present study suggests that increased mechanical loads and blunted neuromuscular responses are both required for the development of obstructive sleep apnea.

obstructive sleep apnea; critical pressure; mechanical properties; neuromuscular control

OBSTRUCTIVE SLEEP APNEA is a prevalent disorder characterized by repetitive upper airway obstruction that results in recurrent hypoxemia and arousal from sleep (32, 48). Although it is well known that pharyngeal occlusion is the primary cause of sleep apnea, the precise mechanisms leading to upper airway obstruction are incompletely understood. Moreover, the critical pressure of the upper airway (Pcrit), a measurement of upper airway collapsibility, is elevated in sleep apnea patients compared with normal subjects during sleep (11, 13, 57, 63). There remains considerable debate as to whether observed differences in pharyngeal collapsibility are due to alterations in anatomically imposed mechanical loads or in dynamic neuromuscular responses (32, 50, 64). Imaging studies in patients with sleep apnea have consistently demonstrated narrowing of the pharynx due to alterations in soft tissues and fat deposition that presumably increase pharyngeal collapsibility (4, 15, 51). However, despite the alterations in pharyngeal anatomy and elevations in pharyngeal collapsibility observed in sleep apnea patients (4, 15, 51), recent evidence suggests that mechanical loads may account for only one-third of the variability in sleep apnea severity (69).

Dynamic neuromuscular control of the upper airway (17, 36, 37, 48, 68) differs between sleep apnea patients and normal subjects, particularly during wakefulness. Specifically, during the waking state, it has been proposed that patients with anatomically narrowed upper airways require increased genioglossal muscle activity to compensate for the narrowing of the upper airway. Dynamic responses to upper airway obstruction can compensate for pharyngeal mechanical loads and stabilize airway patency during both wake and sleep (35, 49, 58–60). Acute upper airway obstruction results in alterations in lung volume, intraluminal airway pressures, and gas exchange, which activate the upper airway musculature and lower pharyngeal collapsibility (30, 35, 49, 58–60). Several investigators have suggested that dynamic responses to upper airway obstruction require coordinated action among numerous rather than any single pharyngeal muscle (2, 9, 25, 26, 52). Despite the presence of extensive literature, the extent to which both mechanical loads and dynamic neuromuscular responses contribute to airflow obstruction has not been systematically evaluated during sleep.

The major goal of this study was to quantitate the effect of upper airway mechanical loads and dynamic responses on sleep apnea pathogenesis. We hypothesized that compared with normal subjects, sleep apnea patients would be characterized by elevations in mechanical loads and impaired dynamic responses to airflow obstruction. In contrast, normal individuals would maintain upper airway patency during sleep due to reduced mechanical loads and/or vigorous dynamic responses in the presence of elevated mechanical loads. We utilized established methods for characterizing upper airway mechanical properties and dynamic responses to airflow obstruction to test these predictions. Since obesity, age, and sex are known risk factors for sleep apnea, our study groups were carefully matched for these factors.


   MATERIALS AND METHODS
TOP
 ABSTRACT
 MATERIALS AND METHODS
RESULTS
 DISCUSSION
 GRANTS
 ACKNOWLEDGMENTS
 REFERENCES
 
Study Population

Patients from the Johns Hopkins Sleep Disorders Center with moderately severe obstructive sleep apnea [apnea-hypopnea index (AHI) > 20 events/h] and a matched normal group of healthy volunteers from the community (AHI < 5 events/h and flow-limited breathing for less than half the night) were recruited. The groups were matched for age, sex, and body mass index (BMI). The study was approved by the institutional review board on human research, and all subjects provided written informed consent.

Assessment of Sleep

An overnight sleep study was performed in subjects to determine the presence or absence of obstructive sleep apnea using techniques previously described (46). Sleep staging and arousals were scored using standard criteria (47). Airflow was monitored using a differential pressure transducer (Validyne ±2 cmH20; Northridge, CA) connected to nasal cannula. Thoracoabdominal movements were monitored using piezoelectrode strain gauges (Sleepmate, Midlothian, VA), and oxyhemoglobin saturation was monitored continuously via pulse oximetry (Ohmeda, Louisville, CO). Signals were acquired digitally scored for obstructive apneas and hypopneas as previously described (46). Severity of obstructive sleep apnea was defined by the AHI as determined by the number of obstructive apneas and hypopneas per hour.

Experimental Set-Up for Pharyngeal Critical Pressure Measurements

Following the baseline sleep study, patients underwent polysomnography while wearing a tight-fitting nasal mask for an additional one to two nights. Signals were amplified (Grass 78D Polygraph, Grass Instruments, Quincy, MA) and acquired digitally (Windaq, Akron, OH). Nasal pressure (PN) was measured continuously at the level of the mask, and airflow measurements were obtained using a pneumotachograph (Hans Rudolph, Kansas City, MO) attached to a differential pressure transducer placed between the nasal mask and a continuous positive airway pressure (CPAP) unit designed to apply pressures between –20 and + 20 cmH2O. We attempted to minimize the undue influence of mask and mouth leaks on the bias flow in the breathing circuit with customized, well-fitted masks and by detecting mouth leak with an oral thermistor. Respiratory effort was monitored via a Hyatt-type esophageal balloon (Ackrad Laboratories, Cranford, NJ) placed perinasally for monitoring esophageal pressure (11 of 16 normal patients and 11 of 16 sleep apnea subjects) and/or a piezoelectrode abdominal strain gauge. Patients slept in the supine position with one pillow under the head during the measurement periods.

Partitioning the Pharyngeal Critical Pressure

After the baseline sleep study, pharyngeal critical pressure measurements were obtained during sleep and partitioned between its mechanical (3, 43, 54) and dynamic neuromuscular components (57, 63), as previously reported. During the passive state, the critical pressure (passive Pcrit) of the hypotonic upper airway reflects its mechanical properties (54). During sleep, reductions in PN are associated with progressive increases in neuromuscular activity (34). Under these conditions, the critical pressure can be obtained and reflects the recruitment of dynamic responses to upper airway obstruction, which prevent airway occlusion (active Pcrit).

Assessment of Passive Critical Pressure

As previously demonstrated by other investigators (19, 54, 65), a hypotonic (passive) state was induced by raising PN to a level sufficient to eliminate inspiratory flow limitation (holding pressure). When inspiratory flow limitation was abolished, reduced neuromuscular activity [based on genioglossal electromyogram (EMGGG) or alae nasi electromyogram (EMGAN) activity] was observed. During stable, non-rapid eye movement (NREM) sleep, the passive Pcrit was determined by abruptly lowering the PN for five breaths and returning to the holding pressure (6, 43). PN was abruptly reduced to increasingly lower PNs after reestablishing at least 1 min of stable sleep at the holding pressure. A minimum of two series of stepwise reductions in PN by 1–2 cmH2O that eventually encompassed zero airflow (Pcrit) was collected (see Fig. 1, passive). If an arousal occurred, the protocol was resumed after subjects reinitiated stage 2 NREM sleep for at least 3 min. Breaths associated with microarousals from sleep were excluded from analyses.


Figure 1
View larger version (20K):
[in this window]
[in a new window]

  		Fig. 1. Polysomnographic recording in a normal subject at a nasal pressure (PN) of –2.5 cmH2O during stable stage 2, non-rapid eye movement (NREM) sleep. Left (passive): PN is abruptly reduced from an elevated holding pressure (bracket A) inducing an obstructive apnea (bracket B; zero airflow with increasing esophageal pressure) during the passive airway state. The drop in PN from holding pressure to –2.5 cmH2O altered the bias flow delivered by the pressure device, resulting in the offset in airflow observed during the apnea. Right (active): PN was reduced in a stepwise fashion by 1–2 cmH2O for at least 10 min of stage 2 NREM sleep. At right, a tracing is displayed of the final 3 min at the same pressure level as during the passive state at left. Increases in inspiratory airflow (inspiration is in the downward direction) are seen in association with increases in tonic and peak phasic genioglossus electromyogram activity during the active state; however, airflow remains flow limited with large esophageal pressure swings. EEG, electroencephalogram, EMGGG, raw genioglossus electromyogram activity; PES, esophageal pressure.

 
Assessment of the Active Critical Pressure

A standardized protocol for assessing the active Pcrit was implemented as previously described (11, 12, 53, 55–57, 63). After at least 3 min of stable stage 2 sleep at holding pressure, the PN was reduced in a step-wise fashion by 1–2 cmH2O and sustained for at least 10 min of NREM sleep before the PN was reduced again. As PN was sequentially lowered, stable flow-limited breathing was first observed (see Fig. 1, active) followed by periods of recurrent hypopneas and apneas. The PN was lowered until apneas developed or the individual developed prolonged awakenings. If a prolonged awakening occurred, the protocol was resumed after patients reinitiated 3 min of stable stage 2 NREM sleep at a slightly higher PN. Analyses were performed during periods of stable stage 2 NREM sleep or if there was a consistent, stable pattern of hypopneas or apneas during stage 1 sleep that prevented the establishment of deeper levels of NREM sleep.

EMGGG Monitoring

To document the differences in neuromuscular activity between the passive and active state, EMGGG was monitored in a subsample of apneic (n = 5) and normal (n = 5) subjects. Two fine wire-hook needles were placed perorally, as previously described (36). The raw EMGGG signal was amplified and band-pass filtered between 30 and 3,000 Hz (Grass 78D Polygraph, Grass Instruments, Quincy, MA) (36). The signal was rectified and integrated (time constant 100 ms) offline for a moving time-average signal (Windaq Advanced CODAS; DATAQ Instruments; Akron, OH). The EMGGG signal was quantified as a percentage of maximal awake maneuver (tongue thrust or maximal tongue protrusion against the maxillary ridge) (36). At least three of each maneuver were obtained until a consistent maximal response was achieved.

Data Analysis

Passive and active pressure-flow analyses.   The passive Pcrit of the upper airway was determined by plotting the maximal inspiratory airflow (VImax) from breaths 2–5 against PN under the hypotonic conditions during stable non-REM sleep (3, 43). Each of these breaths was assessed for the presence or absence of inspiratory airflow limitation. To determine VImax, we determined the level of bias flow in the breathing circuit at each PN level. Our measurement assumed that inspiratory and expiratory tidal volumes are equal over breaths 2–5. Under these circumstances, the mean airflow level will equal the bias flow rate through the breathing circuit. The mean flow rate was then used to establish a baseline zero flow level for measurements of maximal inspiratory airflow. When esophageal pressure measurements were present for a subject, inspiratory flow limitation was defined as the presence of a plateau in inspiratory airflow in association with a continued fall in esophageal pressure by at least 1 cmH2O beyond the onset of the plateau. Flow limitation in the absence of esophageal pressure was determined using established criteria (18, 33). Flow limitation was considered to be present when a flattened flow vs. time contour was visualized. Breaths associated with arousal were excluded from analyses. The flow-limited segment of the VImax vs. PN relationship was identified using a median segmented regression approach as previously described (43). The regression equation was solved for Pcrit (PN at which zero airflow is present; Fig. 2). The resistance upstream to the site of obstruction (passive Rus) was calculated as the inverse of the regression slope (63).


Figure 2
View larger version (10K):
[in this window]
[in a new window]

  		Fig. 2. The analytical approach utilized to analyze the passive and active pressure-flow relationships. The passive and active critical pressure (Pcrit) was determined by linear regression of the corresponding pressure-flow relationship and identifying the PN at zero airflow. The difference between the active and passive Pcrit ({Delta}PcritA–P) was considered as a measure of the strength of dynamic neuromuscular responses to upper airway obstruction. Two additional parameters were extracted that reflect dynamic responses to upper airway obstruction. The first parameter was defined as the difference in pressures between the active and passive conditions at the maximal inspiratory airflow (VImax) transition threshold from steady-state breathing to periodic breathing ({Delta}PA–P). {Delta}PA–P is analogous to {Delta}PcritA–P. The second parameter extracted was the VImax at the transition threshold in the active condition below which periodic breathing was observed (see MATERIALS AND METHODS for additional details).

 
Determination of the active Pcrit was calculated in a similar fashion. When stable flow limitation was present, PN and VImax were obtained from nine breaths at the end of a 10-min period of stable NREM sleep. At PNs where the predominant pattern of breathing was periodic, the last three breaths of the final three hypopneas or apneas at each PN level were sampled. Median segmented regression of VImax vs. PN identified the flow-limited segment of the pressure-flow relationship and provided the active Pcrit and the active Rus (Fig. 2) (11). The difference between the active and passive Pcrit ({Delta}PcritA–P; Fig. 2) was considered as a measure of the strength of dynamic neuromuscular responses to upper airway obstruction.

We recognized that lowering the PN would eventually result in periodic obstructive hypopneas and obstructive apneas and arousals from sleep (24). Under such circumstances, sleep and breathing instabilities may confound our assessment of the dynamic responses to upper airway obstruction and the active Pcrit. To address this issue, we identified a PN transition threshold between periods of stable and unstable sleep and breathing in the active condition. This threshold was defined by the level of PN and corresponding VImax at which instability was observed (Fig. 2, point A). The PN range above the transition threshold was used to define the steady-state portion of the active pressure-flow relationship.

To determine dynamic responses to sustained periods of upper airway obstruction during periods of stable stage 2 sleep and breathing, two parameters were derived. First, the PN at the transition threshold during the active condition was compared with the PN of the passive pressure-flow relationship at the same level of VImax (see Fig. 2, point B; {Delta}PA–P). {Delta}PA–P represented the subject's ability to preserve upper airway patency during steady-state sleep and maintain stable breathing patterns by recruiting dynamic neuromuscular responses (analogous to {Delta}PcritA–P). Second, we examined the level of airflow obstruction required to induce periodic breathing in normal and sleep apnea subjects. The subject's susceptibility to periodic breathing was defined as the level of VImax at the transition threshold at which periodicity breathing began along the active pressure-flow relationship (transition threshold; see Fig. 2, point A).

In one normal subject, measurements of passive and active pressure-flow relationships were obtained during stage 3 sleep due to insufficient data collected during stage 2 sleep. Sensitivity analyses including and excluding the data collected in this subject demonstrated no significant differences in the results. The data for the subject were therefore included for all analyses. In three normal females (see Table 2), the active Pcrit was indeterminate due to awakenings from sleep at negative PN levels. Nevertheless, before the awakenings, subjects 5, 9, and 16 demonstrated partial upper airway obstruction (i.e., inspiratory flow-limitation) at a PN of –7.2, –8.4, and –13.7 cmH2O, respectively. In these three subjects, the active Pcrit was assumed to be equal to the lowest PN associated with flow-limited breathing before arousal. Imputing the active Pcrit with a value that was equal to the lowest PN obtained would increase the average active Pcrit and decrease the {Delta}PcritA–P in the normal group and thus bias against our primary hypothesis. Sensitivity analyses of the active Pcrit and compensatory neuromuscular responses ({Delta}PcritA–P) excluding these three subjects did not change our findings. Therefore, we present the active Pcrit and {Delta}PcritA–P data in RESULTS excluding these three subjects. In contrast, {Delta}PA–P and the VImax at the transition threshold were obtained in the three normal female subjects, whose data were therefore included for analyses.


View this table:
[in this window]
[in a new window]

  		Table 1. Summary of demographic and polysomnography data for normal subjects and OSA patients

 

View this table:
[in this window]
[in a new window]

  		Table 2. Individual demographic and upper airway characteristics of participants

 
EMGGG analyses.   Tonic and peak phasic EMGGG values were determined for breaths at a PN level equivalent to the passive Pcrit during the passive and active conditions (see Fig. 1). Tonic activity was defined as the lowest activity present during expiration. Peak phasic activity was defined as the maximal activity during inspiration. The difference between tonic EMGGG activity during the passive condition (Fig. 1, bracket B) and holding pressure (Fig. 1, bracket A) was calculated. Similarly, the difference between tonic EMGGG activity during the active condition (Fig. 1, active) and holding pressure (Fig. 1, bracket A) was determined. The difference represents the relative activation of upper airway tonic EMGGG during passive or active conditions compared with the holding pressure level. Corresponding calculations were performed to determine the relative activation of peak phasic EMGGG activity during passive or active conditions compared with the holding pressure level. The change in tonic and peak phasic EMGGG activity from the passive to the active state ({Delta}EMGGG,A–P) was also calculated.

Statistical analysis.   All statistical analyses were performed using STATA 8 (Stata, College Station, TX). The primary outcomes in the study were the differences between the active and passive Pcrit ({Delta}PcritA–P), the difference in pressures between the active and passive conditions at the VImax transition threshold ({Delta}PA–P), the passive Pcrit, and the active Pcrit between apneic and normal subjects. Secondary outcomes included differences in Rus, the transition threshold at which periodic breathing began in the active condition, and tonic and peak phasic EMGGG between the two groups. Correlations between tonic or peak phasic {Delta}EMGGG,A–P activity and {Delta}PcritA–P or {Delta}PA–P were examined using Pearson product-moment correlation. In addition, a composite passive and active pressure-flow relationship for normal subjects and sleep apnea patients was created by determining the median slope and intercept for each condition and disease state. Variables were not normally distributed; therefore the Wilcoxon rank-sum test was used for nonpaired comparisons, and the sign-rank test was used for paired comparisons. Chi-square analysis was used to test proportions. Statistical significance was assumed for P < 0.05. Results are presented as means (SD) unless otherwise noted.


   RESULTS
TOP
 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
DISCUSSION
 GRANTS
 ACKNOWLEDGMENTS
 REFERENCES
 
Subject Characteristics

Sixteen subjects with obstructive sleep apnea and sixteen normal subjects were recruited for the study. There were no significant differences between the two groups with respect to demographic characteristics (see Table 1) as planned by study design. Table 1 also demonstrates the sleep study characteristics of the two groups. Sleep apnea patients demonstrated increased stage 1 sleep, decreased stage 2 sleep, an elevated AHI in NREM and REM sleep, and a lower average nadir oxyhemoglobin value in both NREM and REM sleep compared with normal subjects; otherwise the two groups exhibited similar polysomnographic characteristics. In Table 2, subject characteristics and upper airway measurements are presented.

Passive Properties

The results for passive Pcrit are shown for each group in Fig. 3A. The mean (SD) holding pressure was 9.0 (SD 2.3) and 5.7 cmH2O (SD 1.9) (P = 0.0007) for obstructive sleep apnea patients and normal subjects, respectively. The mean passive Pcrit was elevated in patients with obstructive sleep apnea compared with normal subjects [–0.05 (SD 2.4) and –4.5 cmH2O (SD 3.0), respectively; P = 0.0003], suggesting increased airway collapsibility under conditions of reduced neuromuscular activity. However, there was no significant difference in the passive Rus between obstructive sleep apnea patients and normal subjects [18.6 (SD 6.5) and 24.0 cmH2O·l–1·s (SD 15.0), respectively; P = 0.27].


Figure 3
View larger version (11K):
[in this window]
[in a new window]

  		Fig. 3. Passive and active upper airway critical pressure (Pcrit) measurements in patients with obstructive sleep apnea (OSA) and age-, sex-, and body mass index-matched normal subjects. A: data for all subjects. Differences in passive Pcrit and active Pcrit between normal subjects and sleep apnea patients demonstrated statistical significance (P < 0.001). Furthermore, the difference in critical pressure between the passive and active states was greater in normal subjects compared with OSA patients (P < 0.001). B: data for subjects matched on the level of passive Pcrit. Despite comparable passive Pcrit (mechanical loads), normal subjects significantly lowered the active Pcrit further than sleep apnea subjects [{Delta}PcritA–P 8.0 (SD 7.4) vs. 2.2 cmH2O (SD 2.2), respectively, P < 0.05].

 
Active Properties

The mean active Pcrit (see Fig. 3A) was also significantly elevated in obstructive sleep apnea patients compared with normal subjects [–1.6 (SD 3.5) and –11.1 cmH2O (SD 5.3), respectively; P < 0.0001], suggesting increased upper airway collapsibility during conditions of intact neuromuscular activity. There was no significant difference in the active Rus between obstructive sleep apnea patients and normal subjects [22.5 (SD 10.3) and 31.5 cmH2O·l–1·s (SD 18.3), respectively; P = 0.17].

Dynamic Responses to Upper Airway Obstruction

Dynamic responses to upper airway obstruction were greater in normal subjects than sleep apnea patients, as reflected by the {Delta}PcritA–P [–6.9 (SD 5.7) vs. –1.6 cmH2O (SD 2.4), respectively; P = 0.0004] and the {Delta}PA–P [–6.1 (SD 5.4) vs. –0.8 cmH20 (SD 2.2), respectively; P = 0.0006]. The mean passive and active pressure-flow relationships are shown for normal subjects and obstructive sleep apnea patients in Fig. 4.


Figure 4
View larger version (7K):
[in this window]
[in a new window]

  		Fig. 4. Composite passive and active pressure-flow relationships in the normal group and obstructive sleep apnea group. There is a significant left shift in the active pressure-flow relationship seen in the normal group compared with the sleep apnea group, resulting in a lower active critical pressure (active Pcrit) and a less collapsible upper airway. Variability in passive and active Pcrit is represented by SD bars. In addition, the variability in PN and VImax at the transition threshold (threshold at which periodic breathing begins) as represented by SD bars is displayed.

 
To determine whether normal subjects and sleep apnea patients differed in their tendency to develop periodic breathing, we compared VImax at the transition threshold between the two groups (Figs. 2 and 4). VImax at the transition threshold was similar in both groups [263 (SD 62) and 217 ml/s (SD 82) in normal and sleep apnea subjects, respectively; P = 0.08], indicating that both groups developed periodic breathing at comparable levels of airflow obstruction.

Differences in dynamic responses to upper airway obstruction in normal compared with and apneic subjects could be related to differences in upper airway mechanical loads (i.e., passive Pcrit) between the two groups. To address this concern, we compared the dynamic responses to upper airway obstruction in a subgroup of normal subjects [n = 7 (6 male, 1 female); age 41.4 yr (SD 10.2), BMI 29.0 kg/m2 (SD 5.1)] and sleep apnea patients [n = 6 (4 male, 2 female); age 35.3 yr (SD 10.7), BMI 29.8 kg/m2 (SD 5.5); P > 0.2 for all characteristics] with comparable passive Pcrit [–2.0 (SD 2.3) vs. –2.4 cmH2O (SD 1.4), respectively]. Despite comparable mechanical loads (Fig. 3B), the normal subjects had greater dynamic responses to upper airway obstruction than sleep apnea patients as demonstrated by {Delta}PcritA–P [–8.0 (SD 7.4) vs. 2.2 cmH2O (SD 2.2), respectively; P = 0.03] and {Delta}PA–P [–6.0 (SD 3.7) vs. –2.2 cmH2O (SD 2.5), respectively, P = 0.03].

EMGGG Responses

Tonic and peak phasic EMGGG activity were studied in a subsample of obstructive sleep apnea subjects [n = 5 (4 males, 1 female) age 40.2 yr (SD 11.3), BMI 31.4 kg/m2 (SD 6.7)] and normal subjects [n = 5 (4 males, 1 female), age 42.2 yr (SD 13.6), BMI 28.2 kg/m2 (SD 5.9); P > 0.5 for all characteristics]. The change in tonic EMGGG activity between the passive and holding pressure states compared with the change in tonic EMGGG activity between the active and holding pressure states demonstrated significant differences in normal subjects [4.7% (SD 4.2) vs. 14.1% (SD 13.9), P = 0.03] and apneic subjects [2.4% (SD 2.1) vs. 12.7% (SD 9.7), P = 0.03]. Similarly, the change in peak phasic EMGGG activity between passive and holding pressure states compared with the change in peak phasic EMGGG activity between active and holding pressure states demonstrated significant differences in normal subjects [9.7% (SD 4.1) vs. 22.6% (SD 10.8), P = 0.03] and apneic subjects [6.1% (SD 9.1) vs. 31.2% (SD 18.5), P = 0.03]. However, no significant difference in the tonic EMGGG or peak phasic EMGGG activity was found between normal subjects and obstructive sleep apnea patients during either the holding pressure, passive, or active conditions. Furthermore, no significant relationship between any measure of EMGGG activity and {Delta}PcritA–P or {Delta}PA–P was found.


   DISCUSSION
TOP
 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
GRANTS
 ACKNOWLEDGMENTS
 REFERENCES
 
We demonstrated that patients with obstructive sleep apnea were characterized by defects in upper airway mechanical properties and dynamic responses to upper airway obstruction compared with matched normal controls, as evidenced by elevations of upper airway critical pressures under passive and active conditions. In sleep apnea patients, the passive Pcrit was uniformly elevated to levels known to produce severe airflow obstruction during sleep, suggesting that mechanical loads play a key role in the pathogenesis of obstructive sleep apnea. The passive Pcrit was also elevated to a similar degree in a subgroup of our normal subjects, suggesting that some normal individuals have mechanical defects which place them at risk for the disorder. Despite the presence of elevated mechanical loads, these normal subjects demonstrated a markedly greater response in {Delta}PcritA–P and {Delta}PA–P compared with sleep apnea patients who had comparable levels of mechanical loads (passive Pcrit; see Fig. 3B). This finding suggests that neuromuscular responses to airway obstruction improve airway patency during sleep and offset mechanical loads in normal subjects. The present study suggests that increased mechanical loads and blunted neuromuscular responses are both required for the development of obstructive sleep apnea.

Our approach to studying the upper airway during sleep is based on a Starling resistor model in which upper airway patency is determined primarily by the critical pharyngeal pressure (44, 54). The critical pressure has previously been shown to describe a continuum of pharyngeal collapsibility from health to varying degrees of upper airway obstruction including snoring, hypopneas, and apneas (11, 57). As summarized in Fig. 3, in normal sleeping subjects with intact neuromuscular responses, the active Pcrit is markedly negative and the upper airway remains patent compared with obstructive sleep apnea patients in whom the Pcrit is close to atmospheric pressure and the upper airway occludes spontaneously at sleep onset. Furthermore, a range of critical pressures can be obtained that is characterized by a relative threshold above which recurrent apneas and hypopneas begin to occur (apnea-hypopnea threshold; see Fig. 5) (11). The present study confirmed that an active Pcrit greater than approximately –5 cmH2O distinguished most obstructive sleep apnea patients (14 of 16 subjects) from normal subjects (15 of 16 subjects) (11).


Figure 5
View larger version (22K):
[in this window]
[in a new window]

  		Fig. 5. Role of mechanical loads and compensatory neuromuscular responses in the context of critical pressure measurements. As shown in left hand bar with graded shading, approximate levels of Pcrit measurements define a continuum of upper airway collapsibility from health to disease, including normal (Pcrit < –10 cmH2O), snoring (–5 cmH2O < Pcrit < –10 cmH2O), hypopneas (–5 cmH2O < Pcrit < 0 cmH2O), and apneas (Pcrit > 0 cmH2O) (11, 57). A Pcrit of approximately –5 cmH2O represents the apnea-hypopnea threshold, the level above which obstructive hypopneas and apneas will occur. Structural characteristics impose mechanical loads on the upper airway and elevate Pcrit and predispose the upper airway toward obstruction. Intact dynamic neuromuscular responses lower the Pcrit and maintain upper airway patency. In contrast, blunted neuromuscular responses elevate Pcrit and predispose the upper airway toward obstruction. We hypothesize that obstructive sleep apnea is due to a "two-hit" defect in both passive (mechanical) and active (neuromuscular) upper airway properties.

 
In the present study, the passive Pcrit was higher than the apnea-hypopnea threshold in all sleep apnea patients, indicating the presence of increased mechanical loads as previously reported (3, 22, 43, 54, 67). Moreover, the passive Pcrit measurements were comparable to previous values obtained under conditions of minimal or absent neuromuscular tone, suggesting that mechanical loads are similar during the sleep state and anesthesia (5, 6, 20). Elevations in passive Pcrit observed in apneic patients compared with normal subjects cannot be attributed to overt anthropometric differences between the groups since our groups were matched for age, sex, and obesity. Furthermore, subjects with obvious alterations known to increase mechanical loads were excluded (e.g., tonsillar hypertrophy, microagnathia) (39, 66). Nevertheless, subtle structural changes (29, 51) or differences in regional adiposity (16, 38, 61) could explain elevations in upper airway mechanical loads.

A major finding in the present study was the observation that normal subjects could be divided into two subgroups based on the passive Pcrit. Half of the normal subjects demonstrated a passive Pcrit that was below the apnea-hypopnea threshold of –5 cmH2O, which was sufficient to maintain upper airway patency, regardless of their ability to further lower their critical pressure during the active state. In the remaining normal subjects, the passive Pcrit was greater than –5 cmH2O, placing them at risk for obstructive sleep apnea (see Fig. 5). Nevertheless, these normal subjects compensated for increases in mechanical loads by increasing airflow over a wide range of PNs ({Delta}PA–P) during the active condition and by lowering the active Pcrit below the apnea-hypopnea threshold (increased {Delta}PcritA–P). In contrast, obstructive sleep apnea patients failed to compensate for the increased mechanical loads by lowering their Pcrit during the active state and demonstrated reduced {Delta}PA–P and {Delta}PcritA–P responses, suggesting the presence of blunted neuromuscular responses. It is unlikely that blunted neuromuscular responses in sleep apnea patients were due to baseline differences in mechanical loads between the normal and sleep apnea groups because {Delta}PA–P and {Delta}PcritA–P remained low in sleep apnea subjects after matching both groups for the level of mechanical loads (see passive Pcrit, Fig. 3B). The finding suggests that nonmechanical factors, i.e., neuromuscular factors, most likely account for the differences in the {Delta}PA–P and {Delta}PcritA–P between the two groups.

The mechanism for the lack of {Delta}PA–P and {Delta}PcritA–P responses in obstructive sleep apnea patients is unclear. Recent evidence suggests that neuromuscular responses account for approximately two-thirds of the variability in sleep apnea severity (69), suggesting that compensatory neuromuscular mechanisms account for active responses to upper airway obstruction and play a dominant role in preventing upper airway collapse during sleep. As the upper airway collapses during sleep, an integrated compensatory neuromuscular response can maintain airway patency, as reflected by decreases in the active Pcrit. Several mechanisms might account for blunted dynamic responses to airflow obstruction in apneic patients. First, a loss of pharyngeal mechanoreceptors input (1, 23, 28) may result from chronic exposure to upper airway obstruction and mucosal inflammation. Second, neuromuscular activity may be inadequate or waking neuromuscular reflex responses are lost during sleep (17, 36, 37, 48, 68). Third, decreases in ventilatory response to hypercapnia and hypoxemia in sleep apnea patients (10, 14, 27, 42) indicate insensitivity of central chemoreflex pathways that predispose to recurrent airway collapse. Fourth, instability in the chemical control system, as reflected by measurements of loop gain, has been observed in specific strata of sleep apnea patients, which may contribute to recurrent airway obstruction (22, 41, 67, 71).

Recently, it has been suggested that hyperventilation following arousals during sleep may also contribute to upper airway collapse and destabilize breathing patterns due to altered loop gain (21, 70). In the present study, we demonstrated that periodic breathing began at comparable levels of upper airway obstruction in both normal and sleep apnea subjects, indicating a similar propensity for periodic breathing. Moreover, the experimental findings in normal subjects indicate that if the upstream pressure is lowered sufficiently, the normal dynamic neuromuscular responses can be overwhelmed, leading to periodic obstructive hypopneas and apneas, as would occur spontaneously in obstructive sleep apnea patients (11, 24).

In a limited number of sleep apnea patients and normal subjects, we monitored EMGGG activity to document the presence of neuromuscular activation. Although EMGGG activity increased in the active compared with the passive state in both groups, neither tonic nor peak phasic between-group differences were observed, and no relationship between measures of EMGGG activity and {Delta}PcritA–P or {Delta}PA–P was identified in either group. There are several potential explanations for why EMGGG responses did not differ between the two groups and did not correlate with {Delta}PcritA–P or {Delta}PA–P. First, the genioglossus muscle is only one of many muscles controlling pharyngeal patency and may not represent composite neuromuscular responses to airflow obstruction. Second, we only monitored EMG activity in approximately one-third of our subjects and may have inadequate power to discern differences in neuromuscular responses. Third, it is possible that neuromuscular and airflow responses to upper airway obstruction may be dissociated (54), suggesting that the genioglossus activity may reflect rather than control the state of pharyngeal patency (8, 30, 31). Finally, the conversion of electrical activity to pharyngeal muscle pressure may be impaired in sleep apnea patients and could account for the lack of difference in EMGGG between normal and sleep apnea subjects despite differences in dynamic airflow responses.

Our study has several limitations. First, our passive upper airway state represents a hypotonic upper airway rather than an atonic airway. Nevertheless, our passive Pcrit measurements in both normal and sleep apnea subjects are comparable to those reported under the atonic condition (5, 6, 20). Second, recurrent obstructive apneas and hypopneas in the active condition were associated with sleep-wake instability, which might have confounded the active response. Nevertheless, our active Pcrit measurements were made under comparable conditions of NREM sleep in both groups, who exhibited similar susceptibility to (VImax at the transition threshold) (57, 63). Furthermore, evidence for activation was observed over the entire active pressure-flow relationship, regardless of whether sleep and breathing patterns were stable ({Delta}PA–P) or not stable ({Delta}PcritA–P). Third, while subjects were carefully matched for anthropometric and demographic characteristics, it is possible that differences in body fat distribution (16, 38, 61) and/or hormonal status (7, 40, 45, 72) may account for differences in passive and/or active Pcrit between groups.

The major implication of the present study is that defects in both upper airway mechanical (passive Pcrit) and neuromuscular control ({Delta}PcritA–P or {Delta}PA–P) must be present to develop obstructive sleep apnea (Fig. 5). If the mechanical loads on the upper airway result in a passive Pcrit that is greater than approximately –5 cmH2O, obstructive sleep apnea will occur if the individual is unable to adequately recruit neuromuscular responses ({Delta}PcritA–P or {Delta}PA–P) and lower the active Pcrit to below the –5 cmH2O threshold. In contrast, if the mechanical loads result in a passive Pcrit of less than approximately –5 cmH2O, obstructive sleep apnea will not occur, regardless of the neuromuscular responses ({Delta}PcritA–P or {Delta}PA–P) recruited to further lower the active Pcrit. Thus we propose a "two-hit" hypothesis, where defects in both upper airway mechanical and neuromuscular control are necessary for the development of obstructive sleep apnea.

The precise factors causing these mechanical and neuromuscular defects, however, remain undefined. For example, it is known that weight loss leads to substantial decreases in the active Pcrit (53), which account for improvements in sleep apnea severity (62). Nevertheless, it is unclear whether improvements in upper airway function with weight loss are due to mechanical or neuromuscular factors. Given the increasing prevalence of obesity in Western society, a substantial proportion of the general population may be at risk for developing obstructive sleep apnea (73). Our findings lead us to speculate that obesity leads to defects in mechanical and neuromuscular control of upper airway function. In future studies, partitioning of upper airway collapsibility into its mechanical and neuromuscular components could serve to examine the therapeutic effects of pharmacological agents, upper airway surgery, oral appliance devices, and weight loss on upper airway collapsibility and as intermediate physiological traits for studies examining the genetic susceptibility to obstructive sleep apnea.


   GRANTS
TOP
 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 GRANTS
ACKNOWLEDGMENTS
 REFERENCES
 
The study was supported by National Heart, Lung, and Blood Institute Grants HL-50381 and HL-37379 and National Research Service Award Grant HL-68418. The study was also supported by Johns Hopkins Bayview Medical Center General Clinical Research Center Grant M01-RR-02719.


   ACKNOWLEDGMENTS
TOP
 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 GRANTS
 ACKNOWLEDGMENTS
REFERENCES
 
We acknowledge the technical assistance of Luis E. Pichard in data collection and analyses. We also thank Drs. David Pearse and Naresh Punjabi for insightful comments to improve the manuscript.

Present address of J. J. Marx: St. Joseph's Medical Center; 7601 Osler Dr., 2nd Floor, Towson, MD 21204.


   FOOTNOTES
 

Address for reprint requests and other correspondence: S. P. Patil, Johns Hopkins Sleep Disorders Center, Asthma and Allergy Bldg., 5501 Hopkins Bayview Circle, Rm. 4B30A, Baltimore, MD 21224 (e-mail: spatil{at}jhmi.edu)

The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.


   REFERENCES
TOP
 ABSTRACT
 MATERIALS AND METHODS
 RESULTS
 DISCUSSION
 GRANTS
 ACKNOWLEDGMENTS
 REFERENCES
 

  1. Akahoshi T, White DP, Edwards JK, Beauregard J, Shea SA. Phasic mechanoreceptor stimuli can induce phasic activation of upper airway muscles in humans. J Physiol 531: 677–691, 2001.[Abstract/Free Full Text]
  2. Bailey EF, Fregosi RF. Pressure-volume behaviour of the rat upper airway: effects of tongue muscle activation. J Physiol 548: 563–568, 2003.[Abstract/Free Full Text]
  3. Boudewyns A, Punjabi N, Van de Heyning PH, De Backer WA, O'Donnell CP, Schneider H, Smith PL, Schwartz AR. Abbreviated method for assessing upper airway function in obstructive sleep apnea. Chest 118: 1031–1041, 2000.
  4. Bradley TD, Brown IG, Grossman RF, Zamel N, Martinez D, Phillipson EA, Hoffstein V. Pharyngeal size in snorers, nonsnorers, and patients with obstructive sleep apnea. N Engl J Med 315: 1327–1331, 1986.[Abstract]
  5. Eastwood PR, Platt PR, Shepherd K, Maddison K, Hillman DR. Collapsibility of the upper airway at different concentrations of propofol anesthesia. Anesthesiology 103: 470–477, 2005.[CrossRef][ISI][Medline]
  6. Eastwood PR, Szollosi I, Platt PR, Hillman DR. Collapsibility of the upper airway during anesthesia with isoflurane. Anesthesiology 97: 786–793, 2002.[CrossRef][ISI][Medline]
  7. Fitzpatrick M. Leptin and the obesity hypoventilation syndrome: a leap of faith? Thorax 57: 1–2, 2002.[Free Full Text]
  8. Fogel RB, Malhotra A, Pillar G, Edwards JK, Beauregard J, Shea SA, White DP. Genioglossal activation in patients with obstructive sleep apnea versus control subjects. Mechanisms of muscle control. Am J Respir Crit Care Med 164: 2025–2030, 2001.[Abstract/Free Full Text]
  9. Fuller DD, Williams JS, Janssen PL, Fregosi RF. Effect of co-activation of tongue protrudor and retractor muscles on tongue movements and pharyngeal airflow mechanics in the rat. J Physiol 519: 601–613, 1999.[Abstract/Free Full Text]
  10. Garcia-Rio F, Pino JM, Ramirez T, Alvaro D, Alonso A, Villasante C, Villamor J. Inspiratory neural drive response to hypoxia adequately estimates peripheral chemosensitivity in OSAHS patients. Eur Respir J 20: 724–732, 2002.[Abstract/Free Full Text]
  11. Gleadhill IC, Schwartz AR, Schubert N, Wise RA, Permutt S, Smith PL. Upper airway collapsibility in snorers and in patients with obstructive hypopnea and apnea. Am Rev Respir Dis 143: 1300–1303, 1991.[ISI][Medline]
  12. Gold AR, Marcus CL, Dipalo F, Gold MS. Upper airway collapsibility during sleep in upper airway resistance syndrome. Chest 121: 1531–1540, 2002.
  13. Gold AR, Schwartz AR. The pharyngeal critical pressure. The whys and hows of using nasal continuous positive airway pressure diagnostically. Chest 110: 1077–1088, 1996.
  14. Gold AR, Schwartz AR, Wise RA, Smith PL. Pulmonary function and respiratory chemosensitivity in moderately obese patients with sleep apnea. Chest 103: 1325–1329, 1993.
  15. Haponik EF, Smith PL, Bohlman ME, Allen RP, Goldman SM, Bleecker ER. Computerized tomography in obstructive sleep apnea. Correlation of airway size with physiology during sleep and wakefulness. Am Rev Respir Dis 127: 221–226, 1983.[ISI][Medline]
  16. Hoffstein V, Mateika S. Differences in abdominal and neck circumferences in patients with and without obstructive sleep apnoea. Eur Respir J 5: 377–381, 1992.[Abstract]
  17. Horner RL, Innes JA, Morrell MJ, Shea SA, Guz A. The effect of sleep on reflex genioglossus muscle activation by stimuli of negative airway pressure in humans. J Physiol 476: 141–151, 1994.[Abstract/Free Full Text]
  18. Hosselet JJ, Norman RG, Ayappa I, Rapoport DM. Detection of flow limitation with a nasal cannula/pressure transducer system. Am J Respir Crit Care Med 157: 1461–1467, 1998.
  19. Isono S, Morrison DL, Launois SH, Feroah TR, Whitelaw WA, Remmers JE. Static mechanics of the velopharynx of patients with obstructive sleep apnea. J Appl Physiol 75: 148–154, 1993.[Abstract/Free Full Text]
  20. Isono S, Remmers JE, Tanaka A, Sho Y, Sato J, Nishino T. Anatomy of pharynx in patients with obstructive sleep apnea and in normal subjects. J Appl Physiol 82: 1319–1326, 1997.[Abstract/Free Full Text]
  21. Jordan AS, Eckert DJ, Catcheside PG, McEvoy RD. Ventilatory response to brief arousal from non-rapid eye movement sleep is greater in men than in women. Am J Respir Crit Care Med 168: 1512–1519, 2003.[Abstract/Free Full Text]
  22. Jordan AS, Wellman A, Edwards JK, Schory K, Dover L, MacDonald M, Patel SR, Fogel RB, Malhotra A, White DP. Respiratory control stability and upper airway collapsibility in men and women with obstructive sleep apnea. J Appl Physiol 99: 2020–2027, 2005.[Abstract/Free Full Text]
  23. Kimoff RJ, Sforza E, Champagne V, Ofiara L, Gendron D. Upper airway sensation in snoring and obstructive sleep apnea. Am J Respir Crit Care Med 164: 250–255, 2001.[Abstract/Free Full Text]
  24. King ED, O'Donnell CP, Smith PL, Schwartz AR. A model of obstructive sleep apnea in normal humans. Role of the upper airway. Am J Respir Crit Care Med 161: 1979–1984, 2000.[Abstract/Free Full Text]
  25. Kuna ST. Effects of pharyngeal muscle activation on airway size and configuration. Am J Respir Crit Care Med 164: 1236–1241, 2001.[Abstract/Free Full Text]
  26. Kuna ST, Brennick MJ. Effects of pharyngeal muscle activation on airway pressure-area relationships. Am J Respir Crit Care Med 166: 972–977, 2002.[Abstract/Free Full Text]
  27. Kunitomo F, Kimura H, Tatsumi K, Okita S, Tojima H, Kuriyama T, Honda Y. Abnormal breathing during sleep and chemical control of breathing during wakefulness in patients with sleep apnea syndrome. Am Rev Respir Dis 139: 164–169, 1989.[ISI][Medline]
  28. Larsson H, Carlsson-Nordlander B, Lindblad LE, Norbeck O, Svanborg E. Temperature thresholds in the oropharynx of patients with obstructive sleep apnea syndrome. Am Rev Respir Dis 146: 1246–1249, 1992.[ISI][Medline]
  29. Leiter JC. Upper airway shape: is it important in the pathogenesis of obstructive sleep apnea? Am J Respir Crit Care Med 153: 894–898, 1996.[Abstract]
  30. Malhotra A, Pillar G, Fogel R, Beauregard J, Edwards J, White DP. Upper-airway collapsibility: measurements and sleep effects. Chest 120: 156–161, 2001.
  31. Malhotra A, Pillar G, Fogel RB, Beauregard J, Edwards JK, Slamowitz DI, Shea SA, White DP. Genioglossal but not palatal muscle activity relates closely to pharyngeal pressure. Am J Respir Crit Care Med 162: 1058–1062, 2000.[Abstract/Free Full Text]
  32. Malhotra A, White DP. Obstructive sleep apnoea. Lancet 360: 237–245, 2002.[CrossRef][ISI][Medline]
  33. Mansour KF, Rowley JA, Badr MS. Noninvasive determination of upper airway resistance and flow limitation. J Appl Physiol 97: 1840–1848, 2004.[Abstract/Free Full Text]
  34. Mathew OP, Abu-Osba YK, Thach BT. Influence of upper airway pressure changes on genioglossus muscle respiratory activity. J Appl Physiol 52: 438–444, 1982.[Abstract/Free Full Text]
  35. Mayor AH, Schwartz AR, Rowley JA, Willey SJ, Gillespie MB, Smith PL, Robotham JL. Effect of blood pressure changes on air flow dynamics in the upper airway of the decerebrate cat. Anesthesiology 84: 128–134, 1996.[CrossRef][ISI][Medline]
  36. Mezzanotte WS, Tangel DJ, White DP. Waking genioglossal electromyogram in sleep apnea patients versus normal controls (a neuromuscular compensatory mechanism). J Clin Invest 89: 1571–1579, 1992.[ISI][Medline]
  37. Mezzanotte WS, Tangel DJ, White DP. Influence of sleep onset on upper-airway muscle activity in apnea patients versus normal controls. Am J Respir Crit Care Med 153: 1880–1887, 1996.[Abstract]
  38. Millman RP, Carlisle CC, McGarvey ST, Eveloff SE, Levinson PD. Body fat distribution and sleep apnea severity in women. Chest 107: 362–366, 1995.
  39. Moser RJ III, Rajagopal KR. Obstructive sleep apnea in adults with tonsillar hypertrophy. Arch Intern Med 147: 1265–1267, 1987.[Abstract]
  40. O'Donnell CP, Schwartz AR, Smith PL. Upper airway collapsibility: the importance of gender and adiposity. Am J Respir Crit Care Med 162: 1606–1607, 2000.[Free Full Text]
  41. Onal E, Lopata M. Periodic breathing and the pathogenesis of occlusive sleep apneas. Am Rev Respir Dis 126: 676–680, 1982.[ISI][Medline]
  42. Osanai S, Akiba Y, Fujiuchi S, Nakano H, Matsumoto H, Ohsaki Y, Kikuchi K. Depression of peripheral chemosensitivity by a dopaminergic mechanism in patients with obstructive sleep apnoea syndrome. Eur Respir J 13: 418–423, 1999.[Abstract]
  43. Patil SP, Punjabi NM, Schneider H, O'Donnell CP, Smith PL, Schwartz AR. A simplified method for measuring critical pressures during sleep in the clinical setting. Am J Respir Crit Care Med 170: 86–93, 2004.[Abstract/Free Full Text]
  44. Permutt S, Riley RL. Hemodynamics of collapsible vessels with tone: the vascular waterfall. J Appl Physiol 18: 924–932, 1963.[Abstract/Free Full Text]
  45. Popovic RM, White DP. Upper airway muscle activity in normal women: influence of hormonal status. J Appl Physiol 84: 1055–1062, 1998.[Abstract/Free Full Text]
  46. Punjabi NM, O'Hearn DJ, Neubauer DN, Nieto FJ, Schwartz AR, Smith PL, Bandeen-Roche K. Modeling hypersomnolence in sleep-disordered breathing. A novel approach using survival analysis. Am J Respir Crit Care Med 159: 1703–1709, 1999.[Abstract/Free Full Text]
  47. Rechtschaffen A, Kales A. A Manual of Standardized Terminology, Techniques and Scoring System for Sleep Stages of Human Subjects. Bethesda, MD: National Institutes of Health, 1968.
  48. Remmers JE, deGroot WJ, Sauerland EK, Anch AM. Pathogenesis of upper airway occlusion during sleep. J Appl Physiol 44: 931–938, 1978.[Free Full Text]
  49. Rowley JA, Williams BC, Smith PL, Schwartz AR. Neuromuscular activity and upper airway collapsibility. Mechanisms of action in the decerebrate cat. Am J Respir Crit Care Med 156: 515–521, 1997.[Abstract/Free Full Text]
  50. Schwab RJ. Pro: sleep apnea is an anatomic disorder. Am J Respir Crit Care Med 168: 270–271, 2003.[Free Full Text]
  51. Schwab RJ, Gupta KB, Gefter WB, Metzger LJ, Hoffman EA, Pack AI. Upper airway and soft tissue anatomy in normal subjects and patients with sleep-disordered breathing. Significance of the lateral pharyngeal walls. Am J Respir Crit Care Med 152: 1673–1689, 1995.[Abstract]
  52. Schwartz AR, Eisele DW, Hari A, Testerman R, Erickson D, Smith PL. Electrical stimulation of the lingual musculature in obstructive sleep apnea. J Appl Physiol 81: 643–652, 1996.[Abstract/Free Full Text]
  53. Schwartz AR, Gold AR, Schubert N, Stryzak A, Wise RA, Permutt S, Smith PL. Effect of weight loss on upper airway collapsibility in obstructive sleep apnea. Am Rev Respir Dis 144: 494–498, 1991.[ISI][Medline]
  54. Schwartz AR, O'Donnell CP, Baron J, Schubert N, Alam D, Samadi SD, Smith PL. The hypotonic upper airway in obstructive sleep apnea: role of structures and neuromuscular activity. Am J Respir Crit Care Med 157: 1051–1057, 1998.[Abstract/Free Full Text]
  55. Schwartz AR, Schubert N, Rothman W, Godley F, Marsh B, Eisele D, Nadeau J, Permutt L, Gleadhill I, Smith PL. Effect of uvulopalatopharyngoplasty on upper airway collapsibility in obstructive sleep apnea. Am Rev Respir Dis 145: 527–532, 1992.[ISI][Medline]
  56. Schwartz AR, Smith PL, Wise RA, Bankman I, Permutt S. Effect of positive nasal pressure on upper airway pressure-flow relationships. J Appl Physiol 66: 1626–1634, 1989.[Abstract/Free Full Text]
  57. Schwartz AR, Smith PL, Wise RA, Gold AR, Permutt S. Induction of upper airway occlusion in sleeping individuals with subatmospheric nasal pressure. J Appl Physiol 64: 535–542, 1988.[Abstract/Free Full Text]
  58. Schwartz AR, Thut DC, Brower RG, Gauda EB, Roach D, Permutt S, Smith PL. Modulation of maximal inspiratory airflow by neuromuscular activity: effect of CO2. J Appl Physiol 74: 1597–1605, 1993.[Abstract/Free Full Text]
  59. Schwartz AR, Thut DC, Russ B, Seelagy M, Yuan X, Brower RG, Permutt S, Wise RA, Smith PL. Effect of electrical stimulation of the hypoglossal nerve on airflow mechanics in the isolated upper airway. Am Rev Respir Dis 147: 1144–1150, 1993.[ISI][Medline]
  60. Seelagy MM, Schwartz AR, Russ DB, King ED, Wise RA, Smith PL. Reflex modulation of airflow dynamics through the upper airway. J Appl Physiol 76: 2692–2700, 1994.[Abstract/Free Full Text]
  61. Shinohara E, Kihara S, Yamashita S, Yamane M, Nishida M, Arai T, Kotani K, Nakamura T, Takemura K, Matsuzawa Y. Visceral fat accumulation as an important risk factor for obstructive sleep apnoea syndrome in obese subjects. J Intern Med 241: 11–18, 1997.[CrossRef][ISI][Medline]
  62. Smith PL, Gold AR, Meyers DA, Haponik EF, Bleecker ER. Weight loss in mildly to moderately obese patients with obstructive sleep apnea. Ann Intern Med 103: 850–855, 1985.[ISI][Medline]
  63. Smith PL, Wise RA, Gold AR, Schwartz AR, Permutt S. Upper airway pressure-flow relationships in obstructive sleep apnea. J Appl Physiol 64: 789–795, 1988.[Abstract/Free Full Text]
  64. Strohl KP. Con: sleep apnea is not an anatomic disorder. Am J Respir Crit Care Med 168: 271–272, 2003.[Free Full Text]
  65. Strohl KP, Redline S. Nasal CPAP therapy, upper airway muscle activation, and obstructive sleep apnea. Am Rev Respir Dis 134: 555–558, 1986.[ISI][Medline]
  66. Watanabe T, Isono S, Tanaka A, Tanzawa H, Nishino T. Contribution of body habitus and craniofacial characteristics to segmental closing pressures of the passive pharynx in patients with sleep-disordered breathing. Am J Respir Crit Care Med 165: 260–265, 2002.[Abstract/Free Full Text]
  67. Wellman A, Jordan AS, Malhotra A, Fogel RB, Katz ES, Schory K, Edwards JK, White DP. Ventilatory control and airway anatomy in obstructive sleep apnea. Am J Respir Crit Care Med 170: 1225–1232, 2004.[Abstract/Free Full Text]
  68. Wheatley JR, Mezzanotte WS, Tangel DJ, White DP. Influence of sleep on genioglossus muscle activation by negative pressure in normal men. Am Rev Respir Dis 148: 597–605, 1993.[ISI][Medline]
  69. Younes M. Contributions of upper airway mechanics and control mechanisms to severity of obstructive apnea. Am J Respir Crit Care Med 168: 645–658, 2003.[Abstract/Free Full Text]
  70. Younes M. Role of arousals in the pathogenesis of obstructive sleep apnea. Am J Respir Crit Care Med 169: 623–633, 2004.[Abstract/Free Full Text]
  71. Younes M, Ostrowski M, Thompson W, Leslie C, Shewchuk W. Chemical control stability in patients with obstructive sleep apnea. Am J Respir Crit Care Med 163: 1181–1190, 2001.[Abstract/Free Full Text]
  72. Young T, Finn L, Austin D, Peterson A. Menopausal status and sleep-disordered breathing in the Wisconsin Sleep Cohort Study. Am J Respir Crit Care Med 167: 1181–1185, 2003.[Abstract/Free Full Text]
  73. Young T, Peppard PE, Taheri S. Excess weight and sleep-disordered breathing. J Appl Physiol 99: 1592–1599, 2005.[Abstract/Free Full Text]



This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
J. P. Kirkness, A. R. Schwartz, H. Schneider, N. M. Punjabi, J. J. Maly, A. M. Laffan, B. M. McGinley, T. Magnuson, M. Schweitzer, P. L. Smith, et al.
Contribution of male sex, age, and obesity to mechanical instability of the upper airway during sleep
J Appl Physiol, June 1, 2008; 104(6): 1618 - 1624.
[Abstract] [Full Text] [PDF]