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INNOVATIVE METHODOLOGY
Department of Exercise, Sport, and Leisure Studies, University of Tennessee, Knoxville, Tennessee
Submitted 11 July 2005 ; accepted in final form 1 December 2005
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ABSTRACT |
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10, then a walk/run regression equation was used, whereas if the CV was >10, a lifestyle/leisure time physical activity regression was used. In the cross-validation group, the mean estimates using the new algorithm (2-regression model with an inactivity threshold) were within 0.75 metabolic equivalents (METs) of measured METs for each of the activities performed (P 
0.05), which was a substantial improvement over the single-regression models. The new algorithm is more accurate for the prediction of energy expenditure than currently published regression equations using the Actigraph accelerometer. motion sensor; physical activity; oxygen consumption; activity counts variability
Accelerometers are objective measurement tools that allow researchers to estimate how much energy individuals are expending, as well as to quantify the amount time spent in light [<3 metabolic equivalents (METs)], moderate (3–5.99 METs), and vigorous (6 METs) physical activity. The Actigraph (formerly the Manufacturing Technology Incorporated Actigraph, and the Computer Science Applications accelerometer) is a commonly used device for assessing physical activity. Several regression equations have been developed relating the Actigraph activity counts to energy expenditure (EE) (6–8, 11, 13, 17, 18). Theoretically, this allows researchers to estimate total EE over a given period of time. In addition, these regression equations allow researchers to establish cut points (based on counts/min) for classification of light, moderate, and vigorous physical activity.
Over the past 5 yr, there has been a great increase in the number of prediction equations relating the Actigraph activity counts to EE. The current regression equations for estimating EE based on the counts per minute from the Actigraph accelerometer were developed either during walking and running (6–8, 11, 13, 18) or during moderate-intensity lifestyle activities (8, 17). However, these different equations pose a problem for researchers because no single regression line is able to accurately predict EE or time spent in different intensity categories, across a wide range of activities. In addition, all of these equations assume a linear relationship between counts per minute and EE. Previously, it has been shown that regression equations developed on walking and jogging slightly overestimate the energy cost of walking and light activities, whereas they greatly underestimate the energy cost of moderate-intensity lifestyle activities (2). The lifestyle regression equations provide a closer estimate of EE for moderate-intensity activities, but they greatly overestimate the energy cost of sedentary and light activities and underestimate the energy cost of vigorous activities (2).
Using data previously collected in our laboratory, we observed that walking and running can be distinguished from other activities on the basis of variability in the activity counts from the Actigraph. Generally, locomotor activities (i.e., walking and running) yielded more consistent minute-to-minute counts than other activities (e.g., vacuuming, raking leaves, racquetball, sweeping, etc.), which have more erratic movement patterns. Specifically, the variability in minute-to-minute counts was less for walking than for other activities. In addition, we noted that the slope of the regression line relating counts per minute (x-axis) to METs (y-axis) was steeper for walking and running activities than it was for moderate-intensity lifestyle activities, meaning that two separate regression lines should be used for the prediction of these activities.
Thus we hypothesized that by calculating the coefficient of variation (CV) for six 10-s epochs within a 1-min period, we could distinguish walking and running from all other activities. We further hypothesized that by using the appropriate regression line, we could obtain a closer estimate of EE across a wide range of activities. Therefore, the purpose of this study was to develop a new prediction equation for use with the Actigraph accelerometer that would be composed of two regression lines; one for walking and running and one for all other activities. The determination of which line to use was based on the CV of the counts per 10 s over a 1-min period. A secondary purpose was to examine the ability of these equations to predict time spent in light (<3 METs), moderate (3–6 METs), and vigorous (>6 METs) physical activity.
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METHODS |
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Procedures. Participants performed various lifestyle and sporting activities that were broken into three routines.
1) Routine 1: lying, standing, sitting doing computer work, filing articles, walking up and down stairs at a self-selected speed, cycling at a self-selected work rate.
2) Routine 2: walking at 
3 miles/h (mph) around a track, walking at 4 mph around a track, playing one-on-one basketball, playing singles racquetball, running at 5 mph around a track, running at 7 mph around a track.
3) Routine 3: vacuuming, sweeping and/or mopping, washing windows, washing dishes, lawn mowing with a push mower, raking grass and/or leaves.
Twenty participants performed each routine, with most performing only 1 routine. Specifically, two participants performed all three routines, and eight participants performed two routines. Participants performed each activity in a routine for 10 min, with a 1- to 2-min break between each activity. Oxygen consumption (
O2) was measured continuously throughout the routine by indirect calorimetry (Cosmed K4b2, Cosmed, Rome, Italy). Participants wore an Actigraph accelerometer on the right hip for the duration of the routine. For the Cosmed K4b2 and Actigraph, 2 kg was added to account for the added weight of the devices. Routine 1 was performed in the Applied Physiology Laboratory, routine 2 was performed at University facilities, and routine 3 was performed at either the participant's home or the investigator's home. The participants who did not perform routine 1 were asked to sit quietly for 5 min before the start of the routine so that a resting O2 could be measured.
Indirect calorimetry.
The participants wore a Cosmed K4b2 for the duration of each routine. The Cosmed K4b2 weighs 1.5 kg, including the battery and a specially designed harness. The Cosmed K4b2 has been shown to be a valid device compared with the Douglas bag method during cycle ergometry (12). In addition, the present study found that there was close agreement between the measured O2 from the Cosmed K4b2 during the stationary cycling (range: 44–172 W) and the predicted values from the formula of the American College of Sports Medicine's Guidelines for Graded Exercise and Prescription (1) [R2 = 0.917, standard error of estimate (SEE) = 134.1 ml/min, P < 0.05]. Before each test, the oxygen and carbon dioxide analyzers were calibrated according to the manufacturer's instructions. This consisted of performing a room air calibration and a reference gas calibration using 15.93% oxygen and 4.92% carbon dioxide. The flow turbine was then calibrated using a 3.00-liter syringe (Hans-Rudolph). Finally, a delay calibration was performed to adjust for the lag time that occurs between the expiratory flow measurement and the gas analyzers. During each test, a gel seal was used to help prevent air leaks from the face mask.
Actigraph accelerometer. The Actigraph accelerometer (model 7164) is a small (2.0 x 1.6 x 0.6 in.) and lightweight (42.5 g) uniaxial accelerometer and can measure accelerations in the range of 0.05–2 G and a band-limited frequency of 0.25–2.5 Hz. These values correspond to the range in which most human activities are performed. An 8-bit analog-to-digital converter samples at a rate of 10 Hz, and these values are then summed for the specified time period (epoch). If a 1-min epoch is used, the Actigraph can store 22 days worth of data, which is downloaded to a personal computer via a reader interface unit. The Actigraph was worn at waist level at the right anterior axillary line in a nylon pouch that was attached to a belt. The Actigraph was initialized using 1-s epochs, and the time was synchronized with a digital clock so the start time could be synchronized with the Cosmed K4b2. At the conclusion of the test, the Actigraph data were downloaded to a laptop computer for subsequent analysis. The Actigraph accelerometer was calibrated at the start and end of the study. On both occasions, the calibration fell within ±3.5% of the reference value, which is within the manufacturer's standards.
Data analysis.
Breath-by-breath data were collected by the Cosmed K4b2, which was averaged over a 30-s period. For each activity, the O2 (ml/min) was converted to O2 (ml·kg–1·min–1) and then to METs by dividing by 3.5. For each activity, the MET value for minutes 4–9 were averaged and used for the subsequent analysis.
Because of a technical problem with the Cosmed K4b2, 11 of the walking and running trials had to be repeated. For the 11 trials that were repeated, eight of the participants were the same ones who performed the original trials and three were new participants. The walking and running trials were part of a routine that included indoor and outdoor trials, and calibration was conducted indoors. The oxygen analyzer in the K4b2 is affected by the large changes in ambient temperature, and going from a warm environment to a colder outdoor environment will cause it to overestimate expired fraction of oxygen, and thus underestimate O2 (Paolo Brugnoli, Cosmed, Srl, Pavonadi Albano-Rome, Italy), personal communication, September 1, 2005). The carbon dioxide analyzer remains stable when undergoing changes in ambient temperature. The 11 tests that had to be repeated were originally performed in early spring when the outdoor temperature was well below the indoor temperature by 20–30°. The other tests were performed during a period when the outdoor and indoor temperatures were close to each other. The most recent version of the Cosmed K4b2 uses an internal pneumatic modification that adds a miniaturized valve for performing automatic room air calibrations at programmable time intervals correcting for changes in temperature.
The Actigraph accelerometer data were collected in 1-s epochs and were converted to counts per 10 s and counts per minute using a Visual Basic program, written specifically for this study. We chose to use 1-s epochs to allow greater flexibility during our data analysis, but to apply the newly developed method data can be collected in 10-s epochs. The CV was calculated for each minute by using six 10-s epochs. The average CV and the average counts per minute were calculated for minutes 4–9 of each activity.
Statistical treatment. Statistical analyses were carried out using SPSS version 13.0 for windows (SPSS, Chicago, IL). For all analyses, an alpha level of 0.05 was used to indicate statistical significance. All values are reported as means (SD). Independent t-tests were used to examine the difference between genders for anthropometric variables.
Forty-five tests were randomly selected for the development of the new two-regression model, thus leaving 15 tests for cross-validation of the new equation. Because of waist-mounted accelerometers not being able to detect cycling activity, it was excluded from all analyses. Stationary cycling was included to confirm that the Cosmed K4b2 was providing reasonable O2 values. For the group used to develop the new regression equation, each activity performed by an individual was classified on the basis of the CV value of the 10-s counts: CV from 0.1 to 10 (CV 10), and CV of 0 and >10 (CV > 10). During the walking and running, the CV was almost always <10, whereas for the other activities the CV was almost always >10 (Fig. 1). One exception was during activities such as lying, sitting, and standing where the counts per minute could be zero for a full minute, thus giving a CV of 0. In these cases, they were placed in the CV > 10 group for the purpose of developing the regression equation. This was done because these activities more closely resemble lifestyle activities, and it also provided an anchor point for the lifestyle regression line. We also chose to include lawn mowing and stair climbing in the lifestyle regression not only because their CV was >10 but also because they have factors that increase the energy cost of the activity beyond what would be expected for walking and running. Regression analyses were then used to predict METs from the counts per minute for the CV 10 group and the CV > 10 group.
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Modified Bland-Altman plots were used to graphically show the variability in individual error scores (actual METs minus estimated METs) (5). This allowed for the mean error score and the 95% prediction interval to be shown. Devices that display a tight prediction interval around zero are deemed accurate. Data points below zero signify an overestimation, while points above zero signify an underestimation.
To examine time spent in light (<3 METs), moderate (3–6 METs), and vigorous (>6 METs) physical activity, the minute-by-minute values for the Cosmed K4b2 (criterion) and each accelerometer regression formula (estimate) were compared using the entire routine (including structured activities and transition between activities) for each participant in the cross-validation group. A one-way repeated-measures ANOVA was used to detect differences between the Cosmed K4b2 and each accelerometer regression formula. Pairwise comparisons with Bonferroni adjustments were used to locate significant differences when necessary.
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RESULTS |
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10 and activities where the CV was >10. Further examination of the data revealed that a linear regression did not yield the best fit. For example, the linear regression for activities where the CV is 10 significantly underestimated walking at 2 mph as well as running speeds >7 mph. Therefore, we chose an exponential curve for activities where the CV was 10 (Fig. 2). To verify the use of an exponential curve, we plotted the mean counts per minute vs. METs during treadmill walking and running from the study of King et al. (9) in Fig. 2.
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DISCUSSION |
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TOPABSTRACTMETHODSRESULTSDISCUSSIONGRANTSACKNOWLEDGMENTSREFERENCES |
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The new two-regression model had a mean bias for the prediction of EE of 0.1 METs (95% prediction interval; –1.4, 1.5 METs), whereas the next best prediction of EE was with the Swartz equation, which had a bias of –0.4 METs (95% prediction interval; –3.1, 2.4 METs). Thus the new two-regression model results in a significant improvement over current single regression models.
It is important to examine the differences between the new two-regression model and other single linear regression models that are currently being used. To assist in explaining how the new two-equation model is an advancement for the field, we pooled all of our data together and drew in our two-regression model, Freedson's regression line, and Swartz's regression line (Fig. 8). It is clear that no single regression line can accurately predict the energy cost of specific activities. There is a trade-off, with some predicting the energy cost of walking better than others, and others predicting the energy cost of moderate-intensity lifestyle activities more accurately. It can clearly be seen that the new 2-regression model provides a better prediction across all activities.
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Given that ambulatory physical activity is an important component of overall EE, the new approach has the added benefit of being able to distinguish between walking, running, and other activities, which could be useful to researchers. For the discrimination between walking and running, we propose that a threshold of 6,500 counts/min be used. This is similar to the threshold of 6,683 counts/min chosen by Brage et al. (6) in a study which used treadmill walking and running. Epidemiologists can now examine how much walking individuals perform and distinguish it from running and other moderate-intensity lifestyle activities for the purpose of validating "walking" items on questionnaires. In addition, those interested in weight loss interventions can track individuals in walking programs with better accuracy and determine how much walking individuals are doing during unsupervised sessions.
This study provides some insight into how the new two-regression model would work for detecting time spent in light, moderate, and vigorous physical activity. The mean predicted values for time spent in light, moderate, and vigorous physical activity were within 2.1 min of the actual values. This is in contrast to the single-regression equations that may work well for classifying moderate activity but fail elsewhere. These results are in agreement with Strath et al. (16), who found similar over- and underestimations for the single-regression equations during free-living activity. However, our results should be interpreted with caution because the activities were performed in structured bouts lasting 10 min. Future studies are needed to examine the accuracy of the two-regression model during free-living physical activity.
The present study does have strengths and weaknesses. Strengths of the study are that the new two-regression model was developed on a wide range of activities ranging from sedentary behaviors to vigorous exercise. This is in contrast to previous studies that developed single regression equations on a limited number of activities (i.e., walking/running or moderate-intensity lifestyle activities). In addition, this study examined activities outside of the laboratory, which should enhance the generalizability to free-living situations. Limitations of the study include a small cross-validation group, but there was still enough statistical power (>0.9 for 16 of the 17 activities) to find significant differences between the measured and predicted EE values. Future research should be designed to validate this method in a wide range of individuals for 24-h EE (i.e., with doubly labeled water) and with indirect calorimetry using other types of physical activities.
Because 10-s epochs must be used for the newly developed model, researchers should be aware of the storage capacity of their Actigraph accelerometer. An Actigraph model 7164 with 64 kilobits of memory can store 3.5 days of activity data only or 1.8 days of activity and step data, in 10-s epochs. A model 7164 with 256 kilobits of memory can store 15 days of activity data only or 7.5 days of activity and step data in 10-s epochs. However, the new Actigraph GT1M with 1 megabyte of memory can store 60 days of activity data only or 30 days of activity and step data in 10-s epochs.
In conclusion, the new two-regression model, which is based on the counts per minute and variability in counts between 10-s epochs, improves on currently available methods for the prediction of EE (METs). The new method is more accurate on both a group and individual basis and has a bias of 0.1 METs (95% prediction interval of –1.4, 1.5 METs). In addition, this new method has the advantages of being able to distinguish between walking, running, and other activities, and it predicts the energy cost of specific activities with improved accuracy, which should ultimately result in a closer estimate of 24-h EE. Lastly, the new two-regression model shows promise for providing a better estimate of time spent in light, moderate, and vigorous physical activity compared with the single-regression models.
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ACKNOWLEDGMENTS |
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TOPABSTRACTMETHODSRESULTSDISCUSSIONGRANTSACKNOWLEDGMENTSREFERENCES |
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FOOTNOTES |
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The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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REFERENCES |
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, Data points from the present study; 
data points from the study of King et al. (
activities with a CV 0 or >10. Solid lines, new 2-regression model; dashed line with 2 dots, Swartz equation; the dashed line with 1 dot, Freedson MET equation.