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Transdiaphragmatic transport of tracer albumin from peritoneal to pleural liquid measured in rats

Center for Biomedical Engineering, University of Kentucky, Lexington, Kentucky

Submitted 21 June 2005 ; accepted in final form 5 August 2005

In conscious Wistar-Kyoto rats, we studied the uptake of radioactive tracer ¹²⁵I-albumin into the pleural space and circulation after intraperitoneal (IP) injections with 1 or 5 ml of Ringer solution (3 g/dl albumin). Postmortem, we sampled pleural liquid, peritoneal liquid, and blood plasma 2–48 h after IP injection and measured their radioactivity and protein concentration. Tracer concentration was greater in pleural liquid than in plasma 3 h after injection with both IP injection volumes. This behavior indicated transport of tracer through the diaphragm into the pleural space. A dynamic analysis of the tracer uptake with 5-ml IP injections showed that at least 50% of the total pleural flow was via the diaphragm. A similar estimate was derived from an analysis of total protein concentrations. Both estimates were based on restricted pleural capillary filtration and unrestricted transdiaphragmatic transport. The 5-ml IP injections did not change plasma protein concentration but increased pleural and peritoneal protein concentrations from control values by 22 and 30%, respectively. These changes were consistent with a small (8%) increase in capillary filtration and a small (20%) reduction in transdiaphragmatic flow from control values, consistent with the small (3%) decrease in hydration measured in diaphragm muscle. Thus the pleural uptake of tracer via the diaphragm with the IP injections occurred by the near-normal transport of liquid and protein.

fluid transport; conscious rat; pleural liquid and protein transport; reflection coefficient; microvascular exchange

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