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Stimulatory effect of CO₂ on vagal bronchopulmonary C-fiber afferents during airway inflammation

Department of Physiology, University of Kentucky Medical Center, Lexington, Kentucky

Submitted 6 May 2005 ; accepted in final form 21 June 2005

This study investigated 1) whether pulmonary C fibers are activated by a transient increase in the CO₂ concentration of alveolar gas; and 2) if the CO₂ sensitivity of these afferents is altered during airway inflammation. Single-unit pulmonary C-fiber activity was recorded in anesthetized, open-chest rats. Transient alveolar hypercapnia (HPC) was induced by administering a CO₂-enriched gas mixture (25–30% CO₂, 21% O₂, balance N₂) for five to eight breaths, which increased alveolar CO₂ concentration progressively to near or above 13% for 3–5 s and lowered the arterial pH transiently to 7.10 ± 0.05. Our results showed the following. 1) HPC evoked only a mild stimulation in a small fraction (4/47) of pulmonary C fibers, and there was no significant change in fiber activity (change in fiber activity = 0.22 ± 0.16 imp/s; P > 0.1, n = 47). 2) In sharp contrast, after airway exposure to poly-L-lysine, a cationic protein known to induce mucosal injury, the same challenge of transient HPC activated 87.5% of the pulmonary C fibers tested and evoked a distinct stimulatory effect on these afferents (change in fiber activity = 6.59 ± 1.78 imp/s; P < 0. 01, n = 8). 3) Similar potentiation of the C-fiber response to HPC was also observed after acute exposure to ozone (n = 6) and during a constant infusion of inflammatory mediators such as adenosine (n = 15) or prostaglandin E₂ (n = 12). 4) The enhanced C-fiber sensitivity to CO₂ after poly-L-lysine was completely abrogated by infusion of NaHCO₃ (1.82 mmol·kg^–1·min^–1) that prevented the reduction in pH during HPC (n = 6). In conclusion, only a small percentage (<10%) of the bronchopulmonary C fibers exhibit CO₂ sensitivity under control conditions, but alveolar HPC exerts a consistent and pronounced stimulatory effect on the C-fiber endings during airway inflammation. This effect of CO₂ is probably mediated through the action of hydrogen ions.

hydrogen ion; airway mucosal injury; airway hyperreactivity; hypercapnia

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