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J Appl Physiol 99: 1508-1515, 2005. First published June 16, 2005; doi:10.1152/japplphysiol.00375.2005
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Expression of phospholemman and its association with Na+-K+-ATPase in skeletal muscle: effects of aging and exercise training

Justin Reis,1 Lianqin Zhang,1 Steve Cala,3 Korinne N. Jew,2 Lisa C. Mace,2 Linda Chung,1 Russell L. Moore,2 and Yuk-Chow Ng1

1Department of Pharmacology, The Milton S. Hershey Medical Center, College of Medicine, The Pennsylvania State University, Hershey, Pennsylvania; 2Department of Integrative Physiology, University of Colorado, Boulder, Colorado; and 3Program in Molecular and Cellular Cardiology, Department of Medicine, Wayne State University, Detroit, Michigan

Submitted 1 April 2005 ; accepted in final form 10 June 2005

Phospholemman (PLM) is a recently identified accessory protein of the Na+-K+-ATPase (NKA), with a high level of expression in skeletal muscle. The objectives of this study are to characterize the PLM in skeletal muscle and to test the hypothesis that, as an accessory protein of NKA, expression of PLM and its association with the {alpha}-subunits of NKA is regulated during aging and with exercise training. PLM was characterized in skeletal muscle of 6- and 16-mo-old sedentary middle-aged rats (Ms), and the effects of aging and exercise training were studied in Ms, 29-mo-old sedentary senescent, and 29-mo-old treadmill-exercised senescent rats. Expression of PLM was muscle-type dependent, and immunofluorescence study showed that PLM distributed predominantly on the sarcolemmal membrane of the muscle fibers. Anti-PLM antibody reduced activity of NKA, and thus PLM appears to be required for NKA to express its full activity in skeletal muscle. Expression of PLM was not altered with aging but increased after exercise training. Coimmunoprecipitation studies demonstrated that PLM associates with both the {alpha}1- and {alpha}2-subunit isoforms of NKA. Compared with Ms rats, levels of PLM-associated {alpha}1-subunit increased in 29-mo-old sedentary senescent rats, and treadmill exercise has a tendency to partially reverse it. There was no significant change in PLM-associated {alpha}2-subunit with age, and exercise training has a tendency to increase that level. It is concluded that, in skeletal muscle, PLM appears to be a protein integral to the NKA complex and that PLM has the potential to modulate NKA in an isoform-specific and muscle type-dependent manner in aging and after exercise training.

isoform; {alpha}-subunit; coimmunoprecipitation; FXYD proteins; Fischer 344x Brown Norway rats


Address for reprint requests and other correspondence: Y.-C. Ng, Dept. of Pharmacology, College of Medicine, The Pennsylvania State Univ., Milton S. Hershey Medical Center, 500 Univ. Dr., Hershey, PA 17033 (e-mail: ycn1{at}psu.edu)




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