HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free All Versions of this Article: 99/3/995    most recent 00319.2005v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (4) Citing Articles via Google Scholar Google Scholar Articles by Kluess, H. A. Articles by Clifford, P. S. Search for Related Content PubMed PubMed Citation Articles by Kluess, H. A. Articles by Clifford, P. S.

Elevated temperature decreases sensitivity of P2X purinergic receptors in skeletal muscle arteries

Departments of Anesthesiology and Physiology, Medical College of Wisconsin, and Veterans Affairs Medical Center, Milwaukee, Wisconsin

Submitted 21 March 2005 ; accepted in final form 9 May 2005

We hypothesized that elevated temperatures would attenuate but that reduced temperatures would potentiate the tension mediated by vascular P2X purinergic receptors. The femoral arteries of 24 rats were dissected out and placed in modified Krebs-Henseleit buffer. Arteries were cut into 2-mm sections and mounted in organ tissue baths. Maximal tension (g) was measured during a KCl and norepinephrine challenge. Tension was measured during doses of ,-methylene ATP (10^–7 to 10^–3 M), phenylephrine (10^–7 to 10^–4 M), and acetylcholine (10^–9 to 10^–5 M), with tissue bath temperature adjusted to 35, 37, and 41°C. Dose-response curves were fit using nonlinear regression analysis to calculate the EC₅₀ and slope. The peak tension was lower with ,-methylene ATP during 41°C (1.49 ± 0.14 g) compared with 35°C (2.08 ± 0.09 g) and 37°C (1.94 ± 0.09 g; P < 0.05). Slope and EC₅₀ were not affected by temperature. Tension produced by phenylephrine and relaxation to acetylcholine were not affected by temperature. These data indicate that the vasoconstrictor response to ,-methylene ATP is sensitive to temperature. Moderate cooling does not potentiate P2X-mediated vasoconstriction, but elevated temperature attenuates the vasoconstrictor response to P2X purinergic receptors.

cold; adenosine 5'-triphosphate; femoral artery; sympatholysis

This article has been cited by other articles:

				Z. Gao, J. D. Li, L. I. Sinoway, and J. Li Effect of muscle interstitial pH on P2X and TRPV1 receptor-mediated pressor response J Appl Physiol, June 1, 2007; 102(6): 2288 - 2293. [Abstract] [Full Text] [PDF]

				D. S. DeLorey, J. J. Hamann, Z. Valic, H. A. Kluess, P. S. Clifford, and J. B. Buckwalter {alpha}-Adrenergic receptor responsiveness is preserved during prolonged exercise Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H392 - H398. [Abstract] [Full Text] [PDF]

				Z. Gao, V. Kehoe, J. Xing, L. Sinoway, and J. Li Temperature modulates P2X receptor-mediated cardiovascular responses to muscle afferent activation Am J Physiol Heart Circ Physiol, September 1, 2006; 291(3): H1255 - H1261. [Abstract] [Full Text] [PDF]

				D. S. DeLorey, J. J. Hamann, H. A. Kluess, P. S. Clifford, and J. B. Buckwalter {alpha}-Adrenergic receptor-mediated restraint of skeletal muscle blood flow during prolonged exercise J Appl Physiol, May 1, 2006; 100(5): 1563 - 1568. [Abstract] [Full Text] [PDF]