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J Appl Physiol 99: 244-251, 2005. First published February 17, 2005; doi:10.1152/japplphysiol.01292.2004
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Expression and localization of caveolins during postnatal development in rat heart: implication of thyroid hormone

Philippe Ratajczak,1 Patricia Oliviéro,1 Françoise Marotte,1 Frantisek Kolar,2 Bohuslav Ostadal,2 and Jane-Lise Samuel1

1Centre de Recherche Cardiovasculaire Inserm Lariboisière U689, Institut Fédératif de Recherche 139, Hôpital Lariboisière, Paris, France; and 2Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska, Czech Republic

Submitted 17 November 2004 ; accepted in final form 15 February 2005

Caveolins modulate signaling pathways involved in cardiac development. Caveolin-1 exists in two isoforms: the {beta}-isoform derivates from an alternative translational start site that creates a protein truncated by 31 amino acids, mainly expressed in endothelial cells, whereas caveolin-3 is present in muscle cells. Our aim was to define caveolin distribution and expression during cardiac postnatal development using immunofluorescence and Western blotting. Caveolin-3 sarcolemmal labeling appeared as dotted lines from days 1 to 5 and as continuous lines after 14 days of age. Caveolin-3 expression, low at birth, increased (4-fold) to reach a maximum (P < 0.05) by day 5 and then decreased to stabilize in adults. Total caveolin-1 and its {alpha}-isoform were codistributed at birth in endothelial and smooth muscle cells; afterward, only the caveolin-1{alpha} labeling became limited to endothelium. Quantitative analysis indicated a similar temporal pattern of both total caveolin-1 and caveolin-1{alpha} expression, suggesting that caveolin-1{alpha} and -1{beta} are coregulated; the caveolin-1{alpha} levels increased fourfold by day 5 to reach a maximum by day 14 (P < 0.05). Tyrosine-14-caveolin-1 phosphorylation, low at birth, increased suddenly around day 14 (8-fold vs. day 1) and returning afterward to basal level. Because the T3/T4 level is maximal by day 14, caveolin-1 expression/phosphorylation profiles were analyzed in hypothyroid heart. The levels of caveolin-1{alpha} and consequently tyrosine-14-caveolin-1 phosphorylation, but not that of caveolin-3, decreased (50%) in hypothyroid 14-day-old rats. Our data demonstrate that, during postnatal cardiac growth, 1) caveolins are distinctly regulated, and 2) thyroid hormones are involved in caveolin-1{alpha} expression.

membrane microdomain; cardiomyocyte; vessel; coronary artery


Address for reprint requests and other correspondence: S. Jane-Lise, CRCIL U689, IFR139, Hôpital Lariboisière, 41 Boulevard de la Chapelle, 75475 Paris Cedex 10, France (E-mail: janelyse.samuel{at}larib.inserm.fr)




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