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HIGHLIGHTED TOPICS
Biomechanics and Mechanotransduction in Cells and Tissues

High-frequency, low-magnitude vibrations suppress the number of blood vessels per muscle fiber in mouse soleus muscle

¹Department of Biomedical Engineering, University of Virginia Health Sciences Center, Charlottesville, Virginia; and ²Department of Biomedical Engineering, State University of New York at Stony Brook, Stony Brook, New York

Submitted 8 October 2004 ; accepted in final form 18 January 2005

Extremely low-magnitude (0.3 g), high-frequency (30–90 Hz), whole body vibrations can stimulate bone formation and are hypothesized to provide a surrogate for the oscillations of muscle during contraction. Little is known, however, about the potential of these mechanical signals to stimulate adaptive responses in other tissues. The objective of this study was to determine whether low-level mechanical signals produce structural adaptations in the vasculature of skeletal muscle. Eight-week-old male BALB/cByJ (BALB) mice were divided into two experimental groups: mice subjected to low-level, whole body vibrations (45 Hz, 0.3 g) superimposed on normal cage activities for 15 min/day (n = 6), and age-matched controls (n = 7). After the 6-wk experimental protocol, sections from end and mid regions of the soleus muscles were stained with lectin from Bandeiraea Simplicifolia, an endothelial cell marker, and smooth muscle (SM) -actin, a perivascular cell marker. Six weeks of this low-level vibration caused a 29% decrease in the number of lectin-positive vessels per muscle fiber in the end region of the soleus muscle, indicating a significant reduction in the number of capillaries per muscle fibers. Similarly, these vibrations caused a 36% reduction in SM -actin-positive vessels per muscle fiber, indicating a reduction in the number of arterioles and venules. The decreases in lectin- and SM -actin-positive vessels per muscle fiber ratios were not significant in the mid muscle sections. These results demonstrate the sensitivity of the vasculature in mouse skeletal muscle to whole body, low-level mechanical signals.

microcirculation; angiogenesis; musculoskeletal adaptation