HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free All Versions of this Article: 98/5/1777    most recent 01182.2004v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (20) Citing Articles via Google Scholar Google Scholar Articles by Menezes, S. L. S. Articles by Rocco, P. R. M. Search for Related Content PubMed PubMed Citation Articles by Menezes, S. L. S. Articles by Rocco, P. R. M.

Pulmonary and extrapulmonary acute lung injury: inflammatory and ultrastructural analyses

¹Laboratory of Respiration Physiology and ⁵Laboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Centro de Ciências da Saúde, Ilha do Fundão, Rio de Janeiro; ²Laboratory of Immunopharmacology, FIOCRUZ, Rio de Janeiro; ³Department of Thoracic Surgery, Hospital do Cancer AC Camargo, and ⁴Department of Pathology, University of São Paulo, São Paulo, Brazil

Submitted 20 October 2004 ; accepted in final form 13 January 2005

To test whether pulmonary and extrapulmonary acute lung injury (ALI) of identical mechanical compromise would express diverse morphological patterns and immunological pathways. For this purpose, a model of pulmonary (p) and extrapulmonary (exp) ALI with similar functional changes was developed and pulmonary morphology (light and electron microscopy), cytokines levels, and neutrophilic infiltration in the bronchoalveolar lavage fluid (BALF), elastic and collagen fiber content in the alveolar septa, and neutrophil apoptosis in the lung parenchyma were analyzed. BALB/c mice were divided into four groups. In control groups, saline was intratracheally (it, 0.05 ml) instilled and intraperitoneally (ip, 0.5 ml) injected, respectively. In the ALIp and ALIexp groups, mice received E. coli lipopolysaccharide (10 µg it and 125 µg ip, respectively). The changes in lung resistive and viscoelastic pressures and in static elastance, alveolar collapse, and cell content in lung tissue were similar in the ALIp and ALIexp groups. The ALIp group presented a threefold increase in KC (murine function homolog to IL-8) and IL-10 levels in the BALF in relation to ALIexp, whereas IL-6 level showed a twofold increase in ALIp. Neutrophils in the BALF were more frequent in ALIp than in ALIexp. ALIp showed more extensive injury of alveolar epithelium, intact capillary endothelium, and apoptotic neutrophils, whereas the ALIexp group presented interstitial edema and intact type I and II cells and endothelial layer. In conclusion, given the same pulmonary mechanical dysfunction independently of the etiology of ALI, insult in pulmonary epithelium yielded more pronounced inflammatory responses, which induce ultrastructural morphological changes.

pulmonary mechanics; cytokines; apoptosis

This article has been cited by other articles:

				E. Borges, M. Pinheiro, A. Eleto-Silva, M. Caliari, and M. Rodrigues-Machado Glycogen content is affected differently in acute pulmonary and extra-pulmonary lung injury Human and Experimental Toxicology, September 1, 2009; 28(9): 583 - 590. [Abstract] [PDF]

				P. L. Silva, C. S. N. B. Garcia, P. A. Maronas, V. R. Cagido, E. M. Negri, N. R. Damaceno-Rodrigues, G. M. Ventura, P. T. Bozza, W. A. Zin, V. L. Capelozzi, et al. Early short-term versus prolonged low-dose methylprednisolone therapy in acute lung injury Eur. Respir. J., March 1, 2009; 33(3): 634 - 645. [Abstract] [Full Text] [PDF]

				M. Perl, C.-S. Chung, U. Perl, J. Lomas-Neira, M. de Paepe, W. G. Cioffi, and A. Ayala Fas-induced Pulmonary Apoptosis and Inflammation during Indirect Acute Lung Injury Am. J. Respir. Crit. Care Med., September 15, 2007; 176(6): 591 - 601. [Abstract] [Full Text] [PDF]

				M. Ochs Stereological analysis of acute lung injury Eur. Respir. Rev., December 1, 2006; 15(101): 115 - 121. [Abstract] [Full Text] [PDF]

				F. B. Santos, L. K. S. Nagato, N. M. Boechem, E. M. Negri, A. Guimaraes, V. L. Capelozzi, D. S. Faffe, W. A. Zin, and P. R. M. Rocco Time course of lung parenchyma remodeling in pulmonary and extrapulmonary acute lung injury J Appl Physiol, January 1, 2006; 100(1): 98 - 106. [Abstract] [Full Text] [PDF]