Journal of Applied Physiology Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 98: 1745-1752, 2005. First published December 23, 2004; doi:10.1152/japplphysiol.01185.2004
8750-7587/05 $8.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
98/5/1745    most recent
01185.2004v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (24)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yang, Y.
Right arrow Articles by Trappe, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, Y.
Right arrow Articles by Trappe, S.

Time course of myogenic and metabolic gene expression in response to acute exercise in human skeletal muscle

Yifan Yang, Andrew Creer, Bozena Jemiolo, and Scott Trappe

Human Performance Laboratory, Ball State University, Muncie, Indiana

Submitted 21 October 2004 ; accepted in final form 17 December 2004

The aim of this study was to examine the time course activation of select myogenic (MRF4, Myf5, MyoD, myogenin) and metabolic (CD36, CPT1, HKII, and PDK4) genes after an acute bout of resistance (RE) or run (Run) exercise. Six RE subjects [25 ± 4 yr (mean ± SD), 74 ± 14 kg, 1.71 ± 0.11 m] and six Run subjects (25 ± 4 yr, 72 ± 5 kg, 1.81 ± 0.07 m, 63 ± 8 ml·kg–1·min–1) were studied. Eight muscle biopsies were taken from the vastus lateralis (RE) and gastrocnemius (Run) before, immediately after, and 1, 2, 4, 8, 12 and 24 h after exercise. RE increased mRNA of MRF4 (3.7- to 4.5-fold 2–4 h post), MyoD (5.8-fold 8 h post), myogenin (2.6- and 3.5-fold 8–12 h post), HKII (3.6- to 10.5-fold 2–12 h post), and PDK4 (14- to 26-fold 2–8 h post). There were no differences in Myf5, CD36, and CPT1 mRNA levels 0–24 h post-RE. Run increased mRNA of MyoD (5.0- to 8.0-fold), HKII (12- to 16-fold), and PDK4 (32- to 52-fold) at 8–12 h postexercise. There were no differences in MRF4, Myf5, myogenin, CD36 and CPT1 mRNA levels 0–24 h post-Run. These data indicate a myogenic and metabolic gene induction with RE and Run exercise. The timing of the gene induction is variable and generally peaks 4–8 h postexercise with all gene expression not significantly different from the preexercise levels by 24 h postexercise. These data provide basic information for the timing of human muscle biopsy samples for gene-expression studies involving exercise.

MRF4; Myf5; MyoD; myogenin; FAT/CD36; CPT1; HKII; PDK4


Address for reprint requests and other correspondence: S. Trappe, Human Performance Laboratory, Ball State Univ., Muncie, IN 47306 (E-mail: strappe{at}bsu.edu)




This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
L. Deldicque, P. Atherton, R. Patel, D. Theisen, H. Nielens, M. J. Rennie, and M. Francaux
Effects of resistance exercise with and without creatine supplementation on gene expression and cell signaling in human skeletal muscle
J Appl Physiol, February 1, 2008; 104(2): 371 - 378.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
H. Mascher, J. Tannerstedt, T. Brink-Elfegoun, B. Ekblom, T. Gustafsson, and E. Blomstrand
Repeated resistance exercise training induces different changes in mRNA expression of MAFbx and MuRF-1 in human skeletal muscle
Am J Physiol Endocrinol Metab, January 1, 2008; 294(1): E43 - E51.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
T. A. Trappe, C. C. Carroll, B. Jemiolo, S. W. Trappe, S. Dossing, M. Kjaer, and S. P. Magnusson
Cyclooxygenase mRNA expression in human patellar tendon at rest and after exercise
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2008; 294(1): R192 - R199.
[Abstract] [Full Text] [PDF]

Home page
 J. Gerontol. A Biol. Sci. Med. Sci.Home page
U. Raue, D. Slivka, B. Jemiolo, C. Hollon, and S. Trappe
Proteolytic Gene Expression Differs At Rest and After Resistance Exercise Between Young and Old Women
J. Gerontol. A Biol. Sci. Med. Sci., December 1, 2007; 62(12): 1407 - 1412.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J.-s. Kim, J. K. Petrella, J. M. Cross, and M. M. Bamman
Load-mediated downregulation of myostatin mRNA is not sufficient to promote myofiber hypertrophy in humans: a cluster analysis
J Appl Physiol, November 1, 2007; 103(5): 1488 - 1495.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
E. Louis, U. Raue, Y. Yang, B. Jemiolo, and S. Trappe
Time course of proteolytic, cytokine, and myostatin gene expression after acute exercise in human skeletal muscle
J Appl Physiol, November 1, 2007; 103(5): 1744 - 1751.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
G. K. McConell, S. J. Bradley, T. J. Stephens, B. J. Canny, B. A. Kingwell, and R. S. Lee-Young
Skeletal muscle nNOS{micro} protein content is increased by exercise training in humans
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2007; 293(2): R821 - R828.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
M. M. Bamman, J. K. Petrella, J.-s. Kim, D. L. Mayhew, and J. M. Cross
Cluster analysis tests the importance of myogenic gene expression during myofiber hypertrophy in humans
J Appl Physiol, June 1, 2007; 102(6): 2232 - 2239.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
G. D. Wadley and G. K. McConell
Effect of nitric oxide synthase inhibition on mitochondrial biogenesis in rat skeletal muscle
J Appl Physiol, January 1, 2007; 102(1): 314 - 320.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. Eliasson, T. Elfegoun, J. Nilsson, R. Kohnke, B. Ekblom, and E. Blomstrand
Maximal lengthening contractions increase p70 S6 kinase phosphorylation in human skeletal muscle in the absence of nutritional supply
Am J Physiol Endocrinol Metab, December 1, 2006; 291(6): E1197 - E1205.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
Y. Yang, B. Jemiolo, and S. Trappe
Proteolytic mRNA expression in response to acute resistance exercise in human single skeletal muscle fibers
J Appl Physiol, November 1, 2006; 101(5): 1442 - 1450.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
U. Raue, D. Slivka, B. Jemiolo, C. Hollon, and S. Trappe
Myogenic gene expression at rest and after a bout of resistance exercise in young (18-30 yr) and old (80-89 yr) women
J Appl Physiol, July 1, 2006; 101(1): 53 - 59.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
V. G. Coffey, A. Shield, B. J. Canny, K. A. Carey, D. Cameron-Smith, and J. A. Hawley
Interaction of contractile activity and training history on mRNA abundance in skeletal muscle from trained athletes
Am J Physiol Endocrinol Metab, May 1, 2006; 290(5): E849 - E855.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J.-s. Kim, D. J. Kosek, J. K. Petrella, J. M. Cross, and M. M. Bamman
Resting and load-induced levels of myogenic gene transcripts differ between older adults with demonstrable sarcopenia and young men and women
J Appl Physiol, December 1, 2005; 99(6): 2149 - 2158.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. E. Civitarese, M. K. C. Hesselink, A. P. Russell, E. Ravussin, and P. Schrauwen
Glucose ingestion during exercise blunts exercise-induced gene expression of skeletal muscle fat oxidative genes
Am J Physiol Endocrinol Metab, December 1, 2005; 289(6): E1023 - E1029.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2005 by the American Physiological Society.