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J Appl Physiol 97: 1445-1452, 2004. First published May 28, 2004; doi:10.1152/japplphysiol.01074.2003
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Sex-specific changes in platelet aggregation and secretion with sexual maturity in pigs

Muthuvel Jayachandran,1,5 Hiroya Okano,1 Ritu Chatrath,2 Whyte G. Owen,3 Joseph P. McConnell,4 and Virginia M. Miller1,5

Departments of 1Surgery, 2Pediatric Cardiology, 3Hematology, 4Laboratory Medicine, and 5Physiology and Biophysics, Mayo Clinic, Rochester, Minnesota 55905

Submitted 2 October 2003 ; accepted in final form 27 May 2004

Cardiovascular disease may begin early in adolescence. Platelets release factors contributing to vascular disease. Experiments were designed to test the hypothesis that hormonal transitions associated with sexual maturity differentially affect platelet aggregation and secretion in males and females. Platelets were collected from juvenile (2–3 mo) and sexually mature (adult; 5–6 mo) male and female pigs (n = 8/group). Maturation was evidenced by increased weight of reproductive tissue and changes in circulating levels of gonadal hormones. Aggregation to ADP (10 µM) and collagen (6 µg/ml) and ATP secretion to 50 nM thrombin were determined by turbidimetric analysis and bioluminescence, respectively. Total platelet counts, platelet turnover, and mean platelet volume did not change with maturity. Platelet aggregation and ATP secretion decreased in females but increased in males with maturity, whereas total ATP content remained unchanged in platelets from females but increased in platelets from males. Platelet fibrinogen receptor, P-selectin expression, and receptors for sex steroids did not change with sexual maturation. Plasma C-reactive protein and brain-type natriuretic peptide also did not change. Results indicate that changes in platelet aggregation and secretion change with sexual maturity differently in females and males. These observations provide evidence on which clinical studies could be designed to examine platelet characteristics in human children and young adults.

estrogen; platelet activation; sex steroid receptors; testosterone


Address for reprint requests and other correspondence: V. M. Miller, Dept. of Surgery, Physiology and Biophysics, Mayo Clinic Rochester, 200 First St. SW, Rochester, MN 55905 (E-mail: miller.virginia{at}mayo.edu).




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