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1Department of Human Physiology, The Copenhagen Muscle Research Centre, Institute of Exercise and Sport Sciences, University of Copenhagen, DK-2100 Copenhagen; 4Department of Molecular Muscle Biology, The Copenhagen Muscle Research Centre, Rigshospitalet, DK-2200 Copenhagen; 5Institute of Biochemistry, University of Southern Denmark, DK-5230 Odense, Denmark; 2Department of Genetics and Cell Biology, Cardiovascular Research Institute Maastricht, Maastricht University, 6200 Maastricht, The Netherlands; and 3Department of Human Biology and Nutritional Sciences, University of Guelph, Ontario, Canada N1G 2W1
Submitted 1 December 2003 ; accepted in final form 13 May 2004
The protein and mRNA levels of several muscle lipid-binding proteins and the activity and mRNA level of muscle lipoprotein lipase (mLPL) were investigated in healthy, nonobese, nontrained (NT), moderately trained, and endurance-trained (ET) women and men. FAT/CD36 protein level was 49% higher (P < 0.05) in women than in men, irrespective of training status, whereas FAT/CD36 mRNA was only higher (P < 0.05) in women than in men in NT subjects (85%). Plasma membrane-bound fatty acid binding protein (FABPpm) content was higher in ET men compared with all other groups, whereas training status did not affect FABPpm content in women. FABPpm mRNA was higher (P < 0.05) in NT women than in ET women and NT men. mLPL activity was not different between gender, but mLPL mRNA was 160% higher (P < 0.001) in women than in men. mLPL activity was 48% higher (P < 0.05) in ET than in NT subjects, irrespective of gender, in accordance with 49% higher (P < 0.05) mLPL mRNA in ET than in NT subjects. A 90-min exercise bout induced an increase (P < 0.05) in FAT/CD36 mRNA (
25%) and FABPpm mRNA (15%) levels in all groups. The present study demonstrated that, in the NT state, women had higher muscle mRNA levels of several proteins related to muscle lipid metabolism compared with men. In the ET state, only the gender difference in mLPL mRNA persisted. FAT/CD36 protein in muscle was higher in women than in men, irrespective of training status. These findings may help explain gender differences in lipid metabolism and, furthermore, suggest that the balance between gene transcription, translation, and possibly breakdown of several proteins in muscle lipid metabolism depend on gender.
fatty acid binding protein; fatty acid translocase/CD36; lipoprotein lipase; fatty acid transport protein 1; gene expression
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