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Injections of recombinant human erythropoietin increases lactate influx into erythrocytes

¹EA 2991 Sport, Performance, Santé (Now EA 3759 Approche Bio-psycho-sociale du Dopage), Faculté des Sciences du Sport, F-34090 Montpellier; ²EA 701 Physiologie des Interactions, Hôpital Arnaud de Villeneuve, F-34295 Montpellier; and ³EA 2991 Sport, Performance, Santé, Faculté des Sciences du Sport, F-34090 Montpellier, France

Submitted 10 July 2003 ; accepted in final form 9 February 2004

Previous studies showed that erythropoietin not only increases erythrocyte production but is also essential in both the synthesis and the good functioning of several erythrocyte membrane proteins, including band 3. It is still unknown whether anion and/or H⁺ fluxes are modified by erythropoietin. This study aimed to evaluate the effect of recombinant human erythropoietin (rHuEPO) injections on lactate transport into erythrocytes via band 3 and H⁺-monocarboxylate transporter MCT-1, two proteins involved in lactate exchange. Nine athletes received subcutaneous rHuEPO (50 U/kg body mass 3 times a week for 4 wk), and seven athletes received a saline solution (placebo group). All subjects were also supplemented with oral iron and vitamins B₉ and B₁₂. Lactate transport into erythrocytes was studied before and after the rHuEPO treatment at different lactate concentrations (1.6, 8.1, 41, and 81.1 mM). After treatment, MCT-1 lactate uptake was increased at 1.6, 41 (P < 0.01), and 81.1 mM lactate concentration (P < 0.001) although lactate uptake via band 3 and nonionic diffusion were unchanged. MCT-1 maximal velocity increased in the rHuEPO group (P < 0.05), reaching higher values than in the placebo group (P < 0.05) after treatment. Our results show that rHuEPO injections increased MCT-1 lactate influx at low and high lactate concentrations. The increase in MCT-1 maximal velocity suggests that rHuEPO may stimulate MCT-1 synthesis during erythrocyte formation in bone marrow.

lactate uptake; H⁺-monocarboxylate cotransporter; band 3

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