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Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1
Submitted 28 March 2003 ; accepted in final form 15 March 2004
To investigate the hypothesis that short-term submaximal training would result in changes in Na+-K+-ATPase content, activity, and isoform distribution in skeletal muscle, seven healthy, untrained men [peak aerobic power (peak oxygen consumption; 
O2 peak) = 45.6 ml·kg–1·min–1 (SE 5.4)] cycled for 2 h/day at 60–65% O2 peak for 6 days. Muscle tissue, sampled from the vastus lateralis before training (0 days) and after 3 and 6 days of training and analyzed for Na+-K+-ATPase content, as assessed by the vanadate facilitated [3H]ouabain-binding technique, was increased (P < 0.05) at 3 days (294 ± 8.6 pmol/g wet wt) and 6 days (308 ± 15 pmol/g wet wt) of training compared with 0 days (272 ± 9.7 pmol/g wet wt). Maximal Na+-K+-ATPase activity as evaluated by the 3-O-methylfluorescein phosphatase assay was increased (P < 0.05) by 6 days (53.4 ± 5.9 nmol·h–1·mg protein–1) but not by 3 days (35.9 ± 4.5 nmol·h–1·mg protein–1) compared with 0 days (37.8 ± 3.7 nmol·h–1·mg protein–1) of training. Relative isoform distribution, measured by Western blot techniques, indicated increases (P < 0.05) in 
2-content by 3 days and 
1-content by 6 days of training. These results indicate that prolonged aerobic exercise represents a potent stimulus for the rapid adaptation of Na+-K+-ATPase content, isoform, and activity characteristics.
submaximal exercise; sodium-potassium-adenosinetriphosphatase pump; content; activity; isoforms
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