Journal of Applied Physiology AJP: Cell Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 95: 1301-1304, 2003. First published May 16, 2003; doi:10.1152/japplphysiol.00342.2003
8750-7587/03 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
95/3/1301    most recent
00342.2003v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (19)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Turk, J. R.
Right arrow Articles by Sturek, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Turk, J. R.
Right arrow Articles by Sturek, M.

C-reactive protein correlates with macrophage accumulation in coronary arteries of hypercholesterolemic pigs

James R. Turk,1 Jeffery A. Carroll,2 M. Harold Laughlin,1,3 Tom R. Thomas,4 Jennifer Casati,1 Douglas K. Bowles,1,3 and Michael Sturek5

1Department of Biomedical Sciences, 2Animal Physiology Research Unit, Agricultural Research Service, US Department of Agriculture, 3Dalton Cardiovascular Research Center, 4Department of Nutritional Sciences, and 5Departments of Medical Pharmacology and Physiology and Internal Medicine, University of Missouri, Columbia, Missouri 65211

Submitted 7 April 2003 ; accepted in final form 8 May 2003

ABSTRACT

A growing body of evidence supports the hypothesis that C-reactive protein (CRP) is a marker of inflammation in coronary artery disease. The purpose of the present study was to test the hypothesis that CRP correlates with macrophage accumulation during the initial stages of coronary vascular disease. Adult male pigs were fed a normal chow (NF) or a high-fat high-cholesterol (HF) diet for 20 wk. After 20 wk, blood was collected for analyses of interleukin-6 (IL-6), CRP, and lipids. After blood collection, the pigs were euthanized and the right coronary arteries (RCA) were harvested and fixed in neutral buffered formalin. Paraffin-embedded sections of RCA were stained immunohistochemically for CRP, scavenger receptor A (SRA), and monocyte chemoattractant protein-1 (MCP-1). All cholesterol fractions were elevated in the HF vs. the NF group (P < 0.05). There was little or no positive staining for CRP, SRA, or MCP-1 in the RCA of NF pigs, but there was extensive staining in lipidladen macrophage foam cells in the HF pigs. Double staining revealed colocalization of CRP with SRA and CRP with MCP-1 in foam cells. Serum IL-6 was below the assay detection limit in all pigs. Serum CRP correlated directly with plasma total cholesterol (R = 0.727, P = 0.041) and accumulation of SRA-positive macrophages (R = 0.938, P < 0.001) in RCA of HF pigs. We conclude that serum CRP correlates with macrophage accumulation and coronary artery disease in hypercholesterolemic pigs.

hypercholesterolemia; monocyte chemoattractant protein; scavenger receptor A


Address for reprint requests and other correspondence: J. R. Turk, Dept. of Biomedical Sciences, 1600 East Rollins Ave., University of Missouri, Columbia, MO 65211 (E-mail: turkj{at}missouri.edu).




This article has been cited by other articles:


Home page
 Nephrol Dial TransplantHome page
T. Kuji, T. Masaki, L. Li, and A. K. Cheung
Expression of C-reactive protein in myointimal hyperplasia in a porcine arteriovenous graft model
Nephrol. Dial. Transplant., September 1, 2007; 22(9): 2469 - 2475.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
V. J. Dzau, E. M. Antman, H. R. Black, D. L. Hayes, J. E. Manson, J. Plutzky, J. J. Popma, and W. Stevenson
The Cardiovascular Disease Continuum Validated: Clinical Evidence of Improved Patient Outcomes: Part I: Pathophysiology and Clinical Trial Evidence (Risk Factors Through Stable Coronary Artery Disease)
Circulation, December 19, 2006; 114(25): 2850 - 2870.
[Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
H. Fujii, S.-H. Li, P. E. Szmitko, P. W.M. Fedak, and S. Verma
C-Reactive Protein Alters Antioxidant Defenses and Promotes Apoptosis in Endothelial Progenitor Cells
Arterioscler Thromb Vasc Biol, November 1, 2006; 26(11): 2476 - 2482.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
Y. Zhong, S.-H. Li, S.-M. Liu, P. E. Szmitko, X.-Q. He, P. W.M. Fedak, and S. Verma
C-Reactive Protein Upregulates Receptor for Advanced Glycation End Products Expression in Human Endothelial Cells
Hypertension, September 1, 2006; 48(3): 504 - 511.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
B. M. Scirica, D. A. Morrow, S. Verma, S. Devaraj, I. Jialal, B. M. Scirica, D. A. Morrow, S. Verma, S. Devaraj, and I. Jialal
The Verdict Is Still Out
Circulation, May 2, 2006; 113(17): 2128 - 2151.
[Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. K. Henderson, J. R. Turk, J. W. E. Rush, and M. H. Laughlin
Endothelial function in coronary arterioles from pigs with early-stage coronary disease induced by high-fat, high-cholesterol diet: effect of exercise
J Appl Physiol, September 1, 2004; 97(3): 1159 - 1168.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. K. Bowles, C. L. Heaps, J. R. Turk, K. K. Maddali, and E. M. Price
Hypercholesterolemia inhibits L-type calcium current in coronary macro-, not microcirculation
J Appl Physiol, June 1, 2004; 96(6): 2240 - 2248.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2003 by the American Physiological Society.