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1August Krogh Institute and 2Rigshospitalet, Copenhagen Muscle Research Centre, DK-2100 Copenhagen, Denmark
Submitted 30 September 2002 ; accepted in final form 23 April 2003
Increasing evidence suggests that the myogenic regulatory factors (MRFs)
and IGF-I have important roles in the hypertrophy response observed after
mechanical loading. We, therefore, hypothesized that a bout of
heavy-resistance training would affect the MRF and IGF-I mRNA levels in human
skeletal muscle. Six male subjects completed four sets of 6-12 repetitions on
a leg press and knee extensor machine separated by 3 min. Myogenin, MRF4,
MyoD, IGF-IEabc (isoforms a, b, and c) and IGF-IEbc (isoform b and c) mRNA
levels were determined in the vastus lateralis muscle by RT-PCR before
exercise, immediately after, and 1, 2, 6, 24, and 48 h postexercise. Myogenin,
MyoD, and MRF4 mRNA levels were elevated (P < 0.005) by 100-400%
0-24 h postexercise. IGF-IEabc mRNA content decreased (P < 0.005)
by 
44% after 1 and 6 h of recovery. The IGF-IEbc mRNA level was
unaffected. The present study shows that myogenin, MyoD, and MRF4 mRNA levels
are transiently elevated in human skeletal muscle after a single bout of
heavy-resistance training, supporting the idea that the MRFs may be involved
in regulating hypertrophy and/or fiber-type transitions. The results also
suggest that IGF-IEa expression may be downregulated at the mRNA level during
the initial part of recovery from resistance exercise.
hypertrophy; myogenin; mechanical load; satellite cells
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