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J Appl Physiol 95: 279-291, 2003. First published March 14, 2003; doi:10.1152/japplphysiol.00783.2001
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Kinetic analysis of pulmonary neutrophil retention in vivo using the multiple-indicator-dilution technique

Andreas J. Schwab,1 Agnés Salamand,2 Yahye Merhi,1 André Simard,1 and Jocelyn Dupuis2

1McGill University Medical Clinic, Montreal General Hospital, Montreal, Quebec H3G 1A4; and 2Department of Medicine, Montreal Heart Institute and University of Montreal, Montreal, Quebec, Canada H1T 1C8

Submitted 25 July 2001 ; accepted in final form 1 March 2003

Multiple-indicator-dilution experiments were performed in the lungs of 13 anesthetized dogs by simultaneous bolus injection of 111In-labeled neutrophils, 51Cr-labeled red blood cells, and Evans blue-labeled albumin. Concomitant counts of unlabeled neutrophils were similar in pulmonary artery and aortic blood samples, demonstrating a dynamic balance across the lungs in the physiological state. Outflow profiles of labeled neutrophils were analyzed on the basis of a recirculatory pharmacokinetic model of labeled albumin. The outflow profiles of the recovered neutrophils were composed of a throughput component of circulating neutrophils and a component of reversibly marginated neutrophils. They were interpreted by a model incorporating neutrophil margination (transfer coefficient = 0.195 ± 0.081 s-1), rapid demargination (0.054 ± 0.027 s-1), and transfer to a slow marginated pool (0.023 ± 0.018 s-1). It will be interesting to apply the analysis in future studies aimed at determining whether it could be a useful research tool to investigate the interactions between the pulmonary endothelium and neutrophils in physiological and diseased states.

pulmonary circulation; mathematical modeling; microcirculatory exchanges; neutrophil margination; recirculatory model


Address for reprint requests and other correspondence: J. Dupuis, Montreal Heart Institute, 5000 Belanger St. East, Montreal, PQ, Canada H1T 1C8 (E-mail: dupuisj{at}icm.umontreal.ca).




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